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Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes

We demonstrate here for the first time the utility of a monolithically integrated hydrogel ionic diode for label-free quantitative DNA detection and real-time monitoring of nucleic acid amplification. The hydrogel ionic diode presented herein, unlike nanomaterial-based field-effect biosensors, featu...

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Autores principales: Xiong, Chenwei, Li, Jie, Li, Luyao, Chen, Long, Zhang, Rong, Mi, Xianqiang, Liu, Yifan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123263/
https://www.ncbi.nlm.nih.gov/pubmed/35607416
http://dx.doi.org/10.1016/j.mtbio.2022.100281
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author Xiong, Chenwei
Li, Jie
Li, Luyao
Chen, Long
Zhang, Rong
Mi, Xianqiang
Liu, Yifan
author_facet Xiong, Chenwei
Li, Jie
Li, Luyao
Chen, Long
Zhang, Rong
Mi, Xianqiang
Liu, Yifan
author_sort Xiong, Chenwei
collection PubMed
description We demonstrate here for the first time the utility of a monolithically integrated hydrogel ionic diode for label-free quantitative DNA detection and real-time monitoring of nucleic acid amplification. The hydrogel ionic diode presented herein, unlike nanomaterial-based field-effect biosensors, features high cost-effectiveness and convenient fabrication. This is realized by patterning a micrometer-sized heterojunction consisting of adjacent segments of polycationic and polyanionic hydrogels on a microfluidic chip through simple photocuring steps. The integrated diode rectifies ionic currents being sensitive to the charge of DNA adsorbed onto the polycationic chains through electrostatic associations. Based on the mechanism, we show that the ionic biosensor can electrically quantify DNA in a dynamic range relevant to typical nucleic acid amplification assays. Utilizing the device, we demonstrate the evaluation of a PCR assay amplifying a 500-bp DNA fragment of E. coli, an infection-causing pathogen, and real-time in situ monitoring of an isothermal assay amplifying E. coli whole genome. We anticipate that the device could potentially pave the way for miniaturized optics-free platforms for quantifying nucleic acid amplification at point-of-care.
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spelling pubmed-91232632022-05-22 Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes Xiong, Chenwei Li, Jie Li, Luyao Chen, Long Zhang, Rong Mi, Xianqiang Liu, Yifan Mater Today Bio Advanced Materials for Disease Diagnosis edited by Kun Qian; Lin Huang We demonstrate here for the first time the utility of a monolithically integrated hydrogel ionic diode for label-free quantitative DNA detection and real-time monitoring of nucleic acid amplification. The hydrogel ionic diode presented herein, unlike nanomaterial-based field-effect biosensors, features high cost-effectiveness and convenient fabrication. This is realized by patterning a micrometer-sized heterojunction consisting of adjacent segments of polycationic and polyanionic hydrogels on a microfluidic chip through simple photocuring steps. The integrated diode rectifies ionic currents being sensitive to the charge of DNA adsorbed onto the polycationic chains through electrostatic associations. Based on the mechanism, we show that the ionic biosensor can electrically quantify DNA in a dynamic range relevant to typical nucleic acid amplification assays. Utilizing the device, we demonstrate the evaluation of a PCR assay amplifying a 500-bp DNA fragment of E. coli, an infection-causing pathogen, and real-time in situ monitoring of an isothermal assay amplifying E. coli whole genome. We anticipate that the device could potentially pave the way for miniaturized optics-free platforms for quantifying nucleic acid amplification at point-of-care. Elsevier 2022-05-11 /pmc/articles/PMC9123263/ /pubmed/35607416 http://dx.doi.org/10.1016/j.mtbio.2022.100281 Text en © 2022 Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Advanced Materials for Disease Diagnosis edited by Kun Qian; Lin Huang
Xiong, Chenwei
Li, Jie
Li, Luyao
Chen, Long
Zhang, Rong
Mi, Xianqiang
Liu, Yifan
Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes
title Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes
title_full Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes
title_fullStr Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes
title_full_unstemmed Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes
title_short Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes
title_sort label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes
topic Advanced Materials for Disease Diagnosis edited by Kun Qian; Lin Huang
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123263/
https://www.ncbi.nlm.nih.gov/pubmed/35607416
http://dx.doi.org/10.1016/j.mtbio.2022.100281
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