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Phosphoglucomutase 1 contributes to optimal cyst development in Toxoplasma gondii
OBJECTIVE: Toxoplasma gondii is a ubiquitous parasite of medical and veterinary importance; however, there exists no cure for chronic toxoplasmosis. Metabolic enzymes required for the production and maintenance of tissue cysts represent promising targets for novel therapies. Here, we use reverse gen...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123713/ https://www.ncbi.nlm.nih.gov/pubmed/35597992 http://dx.doi.org/10.1186/s13104-022-06073-5 |
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author | Quach, Emily V. Cao, Binh Babacarkhial, Edres Ho, Daniel Sharma, Janak Guiton, Pascale S. |
author_facet | Quach, Emily V. Cao, Binh Babacarkhial, Edres Ho, Daniel Sharma, Janak Guiton, Pascale S. |
author_sort | Quach, Emily V. |
collection | PubMed |
description | OBJECTIVE: Toxoplasma gondii is a ubiquitous parasite of medical and veterinary importance; however, there exists no cure for chronic toxoplasmosis. Metabolic enzymes required for the production and maintenance of tissue cysts represent promising targets for novel therapies. Here, we use reverse genetics to investigate the role of Toxoplasma phosphoglucomutase 1, PGM1, in Toxoplasma growth and cystogenesis. RESULTS: We found that disruption of pgm1 did not significantly affect Toxoplasma intracellular growth and the lytic cycle. pgm1-defective parasites could differentiate into bradyzoites and produced cysts containing amylopectin in vitro. However, cysts produced in the absence of pgm1 were significantly smaller than wildtype. Together, our findings suggest that PGM1 is dispensable for in vitro growth but contributes to optimal Toxoplasma cyst development in vitro, thereby necessitating further investigation into the function of this enzyme in Toxoplasma persistence in its host. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-022-06073-5. |
format | Online Article Text |
id | pubmed-9123713 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-91237132022-05-22 Phosphoglucomutase 1 contributes to optimal cyst development in Toxoplasma gondii Quach, Emily V. Cao, Binh Babacarkhial, Edres Ho, Daniel Sharma, Janak Guiton, Pascale S. BMC Res Notes Research Note OBJECTIVE: Toxoplasma gondii is a ubiquitous parasite of medical and veterinary importance; however, there exists no cure for chronic toxoplasmosis. Metabolic enzymes required for the production and maintenance of tissue cysts represent promising targets for novel therapies. Here, we use reverse genetics to investigate the role of Toxoplasma phosphoglucomutase 1, PGM1, in Toxoplasma growth and cystogenesis. RESULTS: We found that disruption of pgm1 did not significantly affect Toxoplasma intracellular growth and the lytic cycle. pgm1-defective parasites could differentiate into bradyzoites and produced cysts containing amylopectin in vitro. However, cysts produced in the absence of pgm1 were significantly smaller than wildtype. Together, our findings suggest that PGM1 is dispensable for in vitro growth but contributes to optimal Toxoplasma cyst development in vitro, thereby necessitating further investigation into the function of this enzyme in Toxoplasma persistence in its host. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-022-06073-5. BioMed Central 2022-05-21 /pmc/articles/PMC9123713/ /pubmed/35597992 http://dx.doi.org/10.1186/s13104-022-06073-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Note Quach, Emily V. Cao, Binh Babacarkhial, Edres Ho, Daniel Sharma, Janak Guiton, Pascale S. Phosphoglucomutase 1 contributes to optimal cyst development in Toxoplasma gondii |
title | Phosphoglucomutase 1 contributes to optimal cyst development in Toxoplasma gondii |
title_full | Phosphoglucomutase 1 contributes to optimal cyst development in Toxoplasma gondii |
title_fullStr | Phosphoglucomutase 1 contributes to optimal cyst development in Toxoplasma gondii |
title_full_unstemmed | Phosphoglucomutase 1 contributes to optimal cyst development in Toxoplasma gondii |
title_short | Phosphoglucomutase 1 contributes to optimal cyst development in Toxoplasma gondii |
title_sort | phosphoglucomutase 1 contributes to optimal cyst development in toxoplasma gondii |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123713/ https://www.ncbi.nlm.nih.gov/pubmed/35597992 http://dx.doi.org/10.1186/s13104-022-06073-5 |
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