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Comparative analysis of mesenchymal stem cells cultivated in serum free media

Stem cells are attractive candidates for the regeneration of tissue and organ. Mesenchymal stem cells (MSCs) have been extensively investigated for their potential applications in regenerative medicine and cell therapy. For developing effective stem cell therapy, the mass production of consistent qu...

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Autores principales: Lee, Joo Youn, Kang, Min Hee, Jang, Ji Eun, Lee, Jeong Eon, Yang, Yuyeong, Choi, Ji Yong, Kang, Hong Seok, Lee, Uiil, Choung, Ji Woong, Jung, Hyeryeon, Yoon, Young-Chan, Jung, Kyung Hee, Hong, Soon–Sun, Yi, Eugene C., Park, Sang Gyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124186/
https://www.ncbi.nlm.nih.gov/pubmed/35597800
http://dx.doi.org/10.1038/s41598-022-12467-z
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author Lee, Joo Youn
Kang, Min Hee
Jang, Ji Eun
Lee, Jeong Eon
Yang, Yuyeong
Choi, Ji Yong
Kang, Hong Seok
Lee, Uiil
Choung, Ji Woong
Jung, Hyeryeon
Yoon, Young-Chan
Jung, Kyung Hee
Hong, Soon–Sun
Yi, Eugene C.
Park, Sang Gyu
author_facet Lee, Joo Youn
Kang, Min Hee
Jang, Ji Eun
Lee, Jeong Eon
Yang, Yuyeong
Choi, Ji Yong
Kang, Hong Seok
Lee, Uiil
Choung, Ji Woong
Jung, Hyeryeon
Yoon, Young-Chan
Jung, Kyung Hee
Hong, Soon–Sun
Yi, Eugene C.
Park, Sang Gyu
author_sort Lee, Joo Youn
collection PubMed
description Stem cells are attractive candidates for the regeneration of tissue and organ. Mesenchymal stem cells (MSCs) have been extensively investigated for their potential applications in regenerative medicine and cell therapy. For developing effective stem cell therapy, the mass production of consistent quality cells is required. The cell culture medium is the most critical aspect of the mass production of qualified stem cells. Classically, fetal bovine serum (FBS) has been used as a culture supplement for MSCs. Due to the undefined and heterologous composition of animal origin components in FBS, efforts to replace animal-derived components with non-animal-derived substances led to safe serum free media (SFM). Adipose derived mesenchymal stem cells (ADSCs) cultivated in SFM provided a more stable population doubling time (PDT) to later passage and more cells in a shorter time compared to FBS containing media. ADSCs cultivated in SFM had lower cellular senescence, lower immunogenicity, and higher genetic stability than ADSCs cultivated in FBS containing media. Differential expression analysis of mRNAs and proteins showed that the expression of genes related with apoptosis, immune response, and inflammatory response were significantly up-regulated in ADSCs cultivated in FBS containing media. ADSCs cultivated in SFM showed similar therapeutic efficacy in an acute pancreatitis mouse model to ADSCs cultivated in FBS containing media. Consideration of clinical trials, not only pre-clinical trial, suggests that cultivation of MSCs using SFM might offer more safe cell therapeutics as well as repeated administration due to low immunogenicity.
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spelling pubmed-91241862022-05-23 Comparative analysis of mesenchymal stem cells cultivated in serum free media Lee, Joo Youn Kang, Min Hee Jang, Ji Eun Lee, Jeong Eon Yang, Yuyeong Choi, Ji Yong Kang, Hong Seok Lee, Uiil Choung, Ji Woong Jung, Hyeryeon Yoon, Young-Chan Jung, Kyung Hee Hong, Soon–Sun Yi, Eugene C. Park, Sang Gyu Sci Rep Article Stem cells are attractive candidates for the regeneration of tissue and organ. Mesenchymal stem cells (MSCs) have been extensively investigated for their potential applications in regenerative medicine and cell therapy. For developing effective stem cell therapy, the mass production of consistent quality cells is required. The cell culture medium is the most critical aspect of the mass production of qualified stem cells. Classically, fetal bovine serum (FBS) has been used as a culture supplement for MSCs. Due to the undefined and heterologous composition of animal origin components in FBS, efforts to replace animal-derived components with non-animal-derived substances led to safe serum free media (SFM). Adipose derived mesenchymal stem cells (ADSCs) cultivated in SFM provided a more stable population doubling time (PDT) to later passage and more cells in a shorter time compared to FBS containing media. ADSCs cultivated in SFM had lower cellular senescence, lower immunogenicity, and higher genetic stability than ADSCs cultivated in FBS containing media. Differential expression analysis of mRNAs and proteins showed that the expression of genes related with apoptosis, immune response, and inflammatory response were significantly up-regulated in ADSCs cultivated in FBS containing media. ADSCs cultivated in SFM showed similar therapeutic efficacy in an acute pancreatitis mouse model to ADSCs cultivated in FBS containing media. Consideration of clinical trials, not only pre-clinical trial, suggests that cultivation of MSCs using SFM might offer more safe cell therapeutics as well as repeated administration due to low immunogenicity. Nature Publishing Group UK 2022-05-21 /pmc/articles/PMC9124186/ /pubmed/35597800 http://dx.doi.org/10.1038/s41598-022-12467-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Lee, Joo Youn
Kang, Min Hee
Jang, Ji Eun
Lee, Jeong Eon
Yang, Yuyeong
Choi, Ji Yong
Kang, Hong Seok
Lee, Uiil
Choung, Ji Woong
Jung, Hyeryeon
Yoon, Young-Chan
Jung, Kyung Hee
Hong, Soon–Sun
Yi, Eugene C.
Park, Sang Gyu
Comparative analysis of mesenchymal stem cells cultivated in serum free media
title Comparative analysis of mesenchymal stem cells cultivated in serum free media
title_full Comparative analysis of mesenchymal stem cells cultivated in serum free media
title_fullStr Comparative analysis of mesenchymal stem cells cultivated in serum free media
title_full_unstemmed Comparative analysis of mesenchymal stem cells cultivated in serum free media
title_short Comparative analysis of mesenchymal stem cells cultivated in serum free media
title_sort comparative analysis of mesenchymal stem cells cultivated in serum free media
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124186/
https://www.ncbi.nlm.nih.gov/pubmed/35597800
http://dx.doi.org/10.1038/s41598-022-12467-z
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