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Comparative analysis of mesenchymal stem cells cultivated in serum free media
Stem cells are attractive candidates for the regeneration of tissue and organ. Mesenchymal stem cells (MSCs) have been extensively investigated for their potential applications in regenerative medicine and cell therapy. For developing effective stem cell therapy, the mass production of consistent qu...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124186/ https://www.ncbi.nlm.nih.gov/pubmed/35597800 http://dx.doi.org/10.1038/s41598-022-12467-z |
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author | Lee, Joo Youn Kang, Min Hee Jang, Ji Eun Lee, Jeong Eon Yang, Yuyeong Choi, Ji Yong Kang, Hong Seok Lee, Uiil Choung, Ji Woong Jung, Hyeryeon Yoon, Young-Chan Jung, Kyung Hee Hong, Soon–Sun Yi, Eugene C. Park, Sang Gyu |
author_facet | Lee, Joo Youn Kang, Min Hee Jang, Ji Eun Lee, Jeong Eon Yang, Yuyeong Choi, Ji Yong Kang, Hong Seok Lee, Uiil Choung, Ji Woong Jung, Hyeryeon Yoon, Young-Chan Jung, Kyung Hee Hong, Soon–Sun Yi, Eugene C. Park, Sang Gyu |
author_sort | Lee, Joo Youn |
collection | PubMed |
description | Stem cells are attractive candidates for the regeneration of tissue and organ. Mesenchymal stem cells (MSCs) have been extensively investigated for their potential applications in regenerative medicine and cell therapy. For developing effective stem cell therapy, the mass production of consistent quality cells is required. The cell culture medium is the most critical aspect of the mass production of qualified stem cells. Classically, fetal bovine serum (FBS) has been used as a culture supplement for MSCs. Due to the undefined and heterologous composition of animal origin components in FBS, efforts to replace animal-derived components with non-animal-derived substances led to safe serum free media (SFM). Adipose derived mesenchymal stem cells (ADSCs) cultivated in SFM provided a more stable population doubling time (PDT) to later passage and more cells in a shorter time compared to FBS containing media. ADSCs cultivated in SFM had lower cellular senescence, lower immunogenicity, and higher genetic stability than ADSCs cultivated in FBS containing media. Differential expression analysis of mRNAs and proteins showed that the expression of genes related with apoptosis, immune response, and inflammatory response were significantly up-regulated in ADSCs cultivated in FBS containing media. ADSCs cultivated in SFM showed similar therapeutic efficacy in an acute pancreatitis mouse model to ADSCs cultivated in FBS containing media. Consideration of clinical trials, not only pre-clinical trial, suggests that cultivation of MSCs using SFM might offer more safe cell therapeutics as well as repeated administration due to low immunogenicity. |
format | Online Article Text |
id | pubmed-9124186 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-91241862022-05-23 Comparative analysis of mesenchymal stem cells cultivated in serum free media Lee, Joo Youn Kang, Min Hee Jang, Ji Eun Lee, Jeong Eon Yang, Yuyeong Choi, Ji Yong Kang, Hong Seok Lee, Uiil Choung, Ji Woong Jung, Hyeryeon Yoon, Young-Chan Jung, Kyung Hee Hong, Soon–Sun Yi, Eugene C. Park, Sang Gyu Sci Rep Article Stem cells are attractive candidates for the regeneration of tissue and organ. Mesenchymal stem cells (MSCs) have been extensively investigated for their potential applications in regenerative medicine and cell therapy. For developing effective stem cell therapy, the mass production of consistent quality cells is required. The cell culture medium is the most critical aspect of the mass production of qualified stem cells. Classically, fetal bovine serum (FBS) has been used as a culture supplement for MSCs. Due to the undefined and heterologous composition of animal origin components in FBS, efforts to replace animal-derived components with non-animal-derived substances led to safe serum free media (SFM). Adipose derived mesenchymal stem cells (ADSCs) cultivated in SFM provided a more stable population doubling time (PDT) to later passage and more cells in a shorter time compared to FBS containing media. ADSCs cultivated in SFM had lower cellular senescence, lower immunogenicity, and higher genetic stability than ADSCs cultivated in FBS containing media. Differential expression analysis of mRNAs and proteins showed that the expression of genes related with apoptosis, immune response, and inflammatory response were significantly up-regulated in ADSCs cultivated in FBS containing media. ADSCs cultivated in SFM showed similar therapeutic efficacy in an acute pancreatitis mouse model to ADSCs cultivated in FBS containing media. Consideration of clinical trials, not only pre-clinical trial, suggests that cultivation of MSCs using SFM might offer more safe cell therapeutics as well as repeated administration due to low immunogenicity. Nature Publishing Group UK 2022-05-21 /pmc/articles/PMC9124186/ /pubmed/35597800 http://dx.doi.org/10.1038/s41598-022-12467-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Lee, Joo Youn Kang, Min Hee Jang, Ji Eun Lee, Jeong Eon Yang, Yuyeong Choi, Ji Yong Kang, Hong Seok Lee, Uiil Choung, Ji Woong Jung, Hyeryeon Yoon, Young-Chan Jung, Kyung Hee Hong, Soon–Sun Yi, Eugene C. Park, Sang Gyu Comparative analysis of mesenchymal stem cells cultivated in serum free media |
title | Comparative analysis of mesenchymal stem cells cultivated in serum free media |
title_full | Comparative analysis of mesenchymal stem cells cultivated in serum free media |
title_fullStr | Comparative analysis of mesenchymal stem cells cultivated in serum free media |
title_full_unstemmed | Comparative analysis of mesenchymal stem cells cultivated in serum free media |
title_short | Comparative analysis of mesenchymal stem cells cultivated in serum free media |
title_sort | comparative analysis of mesenchymal stem cells cultivated in serum free media |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124186/ https://www.ncbi.nlm.nih.gov/pubmed/35597800 http://dx.doi.org/10.1038/s41598-022-12467-z |
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