m(6)Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation

PCIF1 (phosphorylated CTD interacting factor 1) is the first reported RNA N6,2′-O-dimethyladenosine (m(6)Am) methyltransferase. However, the pathological significance of PCIF1 and m(6)Am modification remains unknown. Here we find that both PCIF1 expression and m(6)Am modification are significantly e...

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Autores principales: Zhuo, Wei, Sun, Meng, Wang, Kun, Zhang, Lu, Li, Kai, Yi, Danyang, Li, Mengjie, Sun, Qiang, Ma, Xixi, Liu, Wei, Teng, Lisong, Yi, Chengqi, Zhou, Tianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Nature Singapore 2022
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124189/
https://www.ncbi.nlm.nih.gov/pubmed/35597784
http://dx.doi.org/10.1038/s41421-022-00395-1
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author Zhuo, Wei
Sun, Meng
Wang, Kun
Zhang, Lu
Li, Kai
Yi, Danyang
Li, Mengjie
Sun, Qiang
Ma, Xixi
Liu, Wei
Teng, Lisong
Yi, Chengqi
Zhou, Tianhua
author_facet Zhuo, Wei
Sun, Meng
Wang, Kun
Zhang, Lu
Li, Kai
Yi, Danyang
Li, Mengjie
Sun, Qiang
Ma, Xixi
Liu, Wei
Teng, Lisong
Yi, Chengqi
Zhou, Tianhua
author_sort Zhuo, Wei
collection PubMed
description PCIF1 (phosphorylated CTD interacting factor 1) is the first reported RNA N6,2′-O-dimethyladenosine (m(6)Am) methyltransferase. However, the pathological significance of PCIF1 and m(6)Am modification remains unknown. Here we find that both PCIF1 expression and m(6)Am modification are significantly elevated in gastric cancer tissues. Increased PCIF1 is associated with gastric cancer progression, and predicts poor prognosis. Silence of PCIF1 inhibits the proliferation and invasion of gastric cancer cells, and suppresses tumor growth and metastasis in mouse model. m(6)Am-seq analysis reveals TM9SF1 (transmembrane 9 superfamily member 1) as a target of PCIF1. PCIF1 modifies TM9SF1 mRNA with m(6)Am leading to decreased TM9SF1 translation. TM9SF1 reverses the effects of PCIF1 on gastric cancer cell aggressiveness. Collectively, our work uncovers an oncogenic function of PCIF1, providing insights into the critical role of m(6)Am modification in cancer progression.
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spelling pubmed-91241892022-05-23 m(6)Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation Zhuo, Wei Sun, Meng Wang, Kun Zhang, Lu Li, Kai Yi, Danyang Li, Mengjie Sun, Qiang Ma, Xixi Liu, Wei Teng, Lisong Yi, Chengqi Zhou, Tianhua Cell Discov Article PCIF1 (phosphorylated CTD interacting factor 1) is the first reported RNA N6,2′-O-dimethyladenosine (m(6)Am) methyltransferase. However, the pathological significance of PCIF1 and m(6)Am modification remains unknown. Here we find that both PCIF1 expression and m(6)Am modification are significantly elevated in gastric cancer tissues. Increased PCIF1 is associated with gastric cancer progression, and predicts poor prognosis. Silence of PCIF1 inhibits the proliferation and invasion of gastric cancer cells, and suppresses tumor growth and metastasis in mouse model. m(6)Am-seq analysis reveals TM9SF1 (transmembrane 9 superfamily member 1) as a target of PCIF1. PCIF1 modifies TM9SF1 mRNA with m(6)Am leading to decreased TM9SF1 translation. TM9SF1 reverses the effects of PCIF1 on gastric cancer cell aggressiveness. Collectively, our work uncovers an oncogenic function of PCIF1, providing insights into the critical role of m(6)Am modification in cancer progression. Springer Nature Singapore 2022-05-21 /pmc/articles/PMC9124189/ /pubmed/35597784 http://dx.doi.org/10.1038/s41421-022-00395-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Zhuo, Wei
Sun, Meng
Wang, Kun
Zhang, Lu
Li, Kai
Yi, Danyang
Li, Mengjie
Sun, Qiang
Ma, Xixi
Liu, Wei
Teng, Lisong
Yi, Chengqi
Zhou, Tianhua
m(6)Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation
title m(6)Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation
title_full m(6)Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation
title_fullStr m(6)Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation
title_full_unstemmed m(6)Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation
title_short m(6)Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation
title_sort m(6)am methyltransferase pcif1 is essential for aggressiveness of gastric cancer cells by inhibiting tm9sf1 mrna translation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124189/
https://www.ncbi.nlm.nih.gov/pubmed/35597784
http://dx.doi.org/10.1038/s41421-022-00395-1
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