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Erythro-myeloid progenitor origin of Hofbauer cells in the early mouse placenta

Hofbauer cells (HBCs) are tissue macrophages of the placenta thought to be important for fetoplacental vascular development and innate immune protection. The developmental origins of HBCs remain unresolved and could implicate functional diversity of HBCs in placenta development and disease. In this...

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Autores principales: Freyer, Laina, Lallemand, Yvan, Dardenne, Pascal, Sommer, Alina, Biton, Anne, Gomez Perdiguero, Elisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124577/
https://www.ncbi.nlm.nih.gov/pubmed/35438172
http://dx.doi.org/10.1242/dev.200104
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author Freyer, Laina
Lallemand, Yvan
Dardenne, Pascal
Sommer, Alina
Biton, Anne
Gomez Perdiguero, Elisa
author_facet Freyer, Laina
Lallemand, Yvan
Dardenne, Pascal
Sommer, Alina
Biton, Anne
Gomez Perdiguero, Elisa
author_sort Freyer, Laina
collection PubMed
description Hofbauer cells (HBCs) are tissue macrophages of the placenta thought to be important for fetoplacental vascular development and innate immune protection. The developmental origins of HBCs remain unresolved and could implicate functional diversity of HBCs in placenta development and disease. In this study, we used flow cytometry and paternally inherited reporters to phenotype placenta macrophages and to identify fetal-derived HBCs and placenta-associated maternal macrophages in the mouse. In vivo pulse-labeling traced the ontogeny of HBCs from yolk sac-derived erythro-myeloid progenitors, with a minor contribution from fetal hematopoietic stem cells later on. Single-cell RNA-sequencing revealed transcriptional similarities between placenta macrophages and erythro-myeloid progenitor-derived fetal liver macrophages and microglia. As with other fetal tissue macrophages, HBCs were dependent on the transcription factor Pu.1, the loss-of-function of which in embryos disrupted fetoplacental labyrinth morphology, supporting a role for HBC in labyrinth angiogenesis and/or remodeling. HBC were also sensitive to Pu.1 (Spi1) haploinsufficiency, which caused an initial deficiency in the numbers of macrophages in the early mouse placenta. These results provide groundwork for future investigation into the relationship between HBC ontogeny and function in placenta pathophysiology.
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spelling pubmed-91245772022-06-04 Erythro-myeloid progenitor origin of Hofbauer cells in the early mouse placenta Freyer, Laina Lallemand, Yvan Dardenne, Pascal Sommer, Alina Biton, Anne Gomez Perdiguero, Elisa Development Research Report Hofbauer cells (HBCs) are tissue macrophages of the placenta thought to be important for fetoplacental vascular development and innate immune protection. The developmental origins of HBCs remain unresolved and could implicate functional diversity of HBCs in placenta development and disease. In this study, we used flow cytometry and paternally inherited reporters to phenotype placenta macrophages and to identify fetal-derived HBCs and placenta-associated maternal macrophages in the mouse. In vivo pulse-labeling traced the ontogeny of HBCs from yolk sac-derived erythro-myeloid progenitors, with a minor contribution from fetal hematopoietic stem cells later on. Single-cell RNA-sequencing revealed transcriptional similarities between placenta macrophages and erythro-myeloid progenitor-derived fetal liver macrophages and microglia. As with other fetal tissue macrophages, HBCs were dependent on the transcription factor Pu.1, the loss-of-function of which in embryos disrupted fetoplacental labyrinth morphology, supporting a role for HBC in labyrinth angiogenesis and/or remodeling. HBC were also sensitive to Pu.1 (Spi1) haploinsufficiency, which caused an initial deficiency in the numbers of macrophages in the early mouse placenta. These results provide groundwork for future investigation into the relationship between HBC ontogeny and function in placenta pathophysiology. The Company of Biologists Ltd 2022-04-22 /pmc/articles/PMC9124577/ /pubmed/35438172 http://dx.doi.org/10.1242/dev.200104 Text en © 2022. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Report
Freyer, Laina
Lallemand, Yvan
Dardenne, Pascal
Sommer, Alina
Biton, Anne
Gomez Perdiguero, Elisa
Erythro-myeloid progenitor origin of Hofbauer cells in the early mouse placenta
title Erythro-myeloid progenitor origin of Hofbauer cells in the early mouse placenta
title_full Erythro-myeloid progenitor origin of Hofbauer cells in the early mouse placenta
title_fullStr Erythro-myeloid progenitor origin of Hofbauer cells in the early mouse placenta
title_full_unstemmed Erythro-myeloid progenitor origin of Hofbauer cells in the early mouse placenta
title_short Erythro-myeloid progenitor origin of Hofbauer cells in the early mouse placenta
title_sort erythro-myeloid progenitor origin of hofbauer cells in the early mouse placenta
topic Research Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124577/
https://www.ncbi.nlm.nih.gov/pubmed/35438172
http://dx.doi.org/10.1242/dev.200104
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