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Erythro-myeloid progenitor origin of Hofbauer cells in the early mouse placenta
Hofbauer cells (HBCs) are tissue macrophages of the placenta thought to be important for fetoplacental vascular development and innate immune protection. The developmental origins of HBCs remain unresolved and could implicate functional diversity of HBCs in placenta development and disease. In this...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124577/ https://www.ncbi.nlm.nih.gov/pubmed/35438172 http://dx.doi.org/10.1242/dev.200104 |
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author | Freyer, Laina Lallemand, Yvan Dardenne, Pascal Sommer, Alina Biton, Anne Gomez Perdiguero, Elisa |
author_facet | Freyer, Laina Lallemand, Yvan Dardenne, Pascal Sommer, Alina Biton, Anne Gomez Perdiguero, Elisa |
author_sort | Freyer, Laina |
collection | PubMed |
description | Hofbauer cells (HBCs) are tissue macrophages of the placenta thought to be important for fetoplacental vascular development and innate immune protection. The developmental origins of HBCs remain unresolved and could implicate functional diversity of HBCs in placenta development and disease. In this study, we used flow cytometry and paternally inherited reporters to phenotype placenta macrophages and to identify fetal-derived HBCs and placenta-associated maternal macrophages in the mouse. In vivo pulse-labeling traced the ontogeny of HBCs from yolk sac-derived erythro-myeloid progenitors, with a minor contribution from fetal hematopoietic stem cells later on. Single-cell RNA-sequencing revealed transcriptional similarities between placenta macrophages and erythro-myeloid progenitor-derived fetal liver macrophages and microglia. As with other fetal tissue macrophages, HBCs were dependent on the transcription factor Pu.1, the loss-of-function of which in embryos disrupted fetoplacental labyrinth morphology, supporting a role for HBC in labyrinth angiogenesis and/or remodeling. HBC were also sensitive to Pu.1 (Spi1) haploinsufficiency, which caused an initial deficiency in the numbers of macrophages in the early mouse placenta. These results provide groundwork for future investigation into the relationship between HBC ontogeny and function in placenta pathophysiology. |
format | Online Article Text |
id | pubmed-9124577 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-91245772022-06-04 Erythro-myeloid progenitor origin of Hofbauer cells in the early mouse placenta Freyer, Laina Lallemand, Yvan Dardenne, Pascal Sommer, Alina Biton, Anne Gomez Perdiguero, Elisa Development Research Report Hofbauer cells (HBCs) are tissue macrophages of the placenta thought to be important for fetoplacental vascular development and innate immune protection. The developmental origins of HBCs remain unresolved and could implicate functional diversity of HBCs in placenta development and disease. In this study, we used flow cytometry and paternally inherited reporters to phenotype placenta macrophages and to identify fetal-derived HBCs and placenta-associated maternal macrophages in the mouse. In vivo pulse-labeling traced the ontogeny of HBCs from yolk sac-derived erythro-myeloid progenitors, with a minor contribution from fetal hematopoietic stem cells later on. Single-cell RNA-sequencing revealed transcriptional similarities between placenta macrophages and erythro-myeloid progenitor-derived fetal liver macrophages and microglia. As with other fetal tissue macrophages, HBCs were dependent on the transcription factor Pu.1, the loss-of-function of which in embryos disrupted fetoplacental labyrinth morphology, supporting a role for HBC in labyrinth angiogenesis and/or remodeling. HBC were also sensitive to Pu.1 (Spi1) haploinsufficiency, which caused an initial deficiency in the numbers of macrophages in the early mouse placenta. These results provide groundwork for future investigation into the relationship between HBC ontogeny and function in placenta pathophysiology. The Company of Biologists Ltd 2022-04-22 /pmc/articles/PMC9124577/ /pubmed/35438172 http://dx.doi.org/10.1242/dev.200104 Text en © 2022. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Report Freyer, Laina Lallemand, Yvan Dardenne, Pascal Sommer, Alina Biton, Anne Gomez Perdiguero, Elisa Erythro-myeloid progenitor origin of Hofbauer cells in the early mouse placenta |
title | Erythro-myeloid progenitor origin of Hofbauer cells in the early mouse placenta |
title_full | Erythro-myeloid progenitor origin of Hofbauer cells in the early mouse placenta |
title_fullStr | Erythro-myeloid progenitor origin of Hofbauer cells in the early mouse placenta |
title_full_unstemmed | Erythro-myeloid progenitor origin of Hofbauer cells in the early mouse placenta |
title_short | Erythro-myeloid progenitor origin of Hofbauer cells in the early mouse placenta |
title_sort | erythro-myeloid progenitor origin of hofbauer cells in the early mouse placenta |
topic | Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124577/ https://www.ncbi.nlm.nih.gov/pubmed/35438172 http://dx.doi.org/10.1242/dev.200104 |
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