Determination of tobramycin in eye drops with an open-source hardware ion mobility spectrometer

The analysis of tobramycin was demonstrated successfully as an example for electrospray ionization on an open-source hardware ion mobility spectrometer. This instrument was assembled inexpensively in-house, and required only very few purpose-made components. The quantitative determination of tobramy...

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Detalles Bibliográficos
Autores principales: Chantipmanee, Nattapong, Hauser, Peter C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124657/
https://www.ncbi.nlm.nih.gov/pubmed/35381854
http://dx.doi.org/10.1007/s00216-022-04050-2
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author Chantipmanee, Nattapong
Hauser, Peter C.
author_facet Chantipmanee, Nattapong
Hauser, Peter C.
author_sort Chantipmanee, Nattapong
collection PubMed
description The analysis of tobramycin was demonstrated successfully as an example for electrospray ionization on an open-source hardware ion mobility spectrometer. This instrument was assembled inexpensively in-house, and required only very few purpose-made components. The quantitative determination of tobramycin required 20 s for a reading. The calibration curve for the range from 50 to 200 μM was found to be linear with a correlation coefficient of r = 0.9994. A good reproducibility was obtained (3% relative standard deviation) and the limit of detection was determined as 8 μM. As the concentration of the active ingredient in the eye drops (ophthalmic solutions) is too high for the sensitivity of the instrument, the samples had to be diluted appropriately. GRAPHICAL ABSTRACT: [Image: see text]
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spelling pubmed-91246572022-05-24 Determination of tobramycin in eye drops with an open-source hardware ion mobility spectrometer Chantipmanee, Nattapong Hauser, Peter C. Anal Bioanal Chem Research Paper The analysis of tobramycin was demonstrated successfully as an example for electrospray ionization on an open-source hardware ion mobility spectrometer. This instrument was assembled inexpensively in-house, and required only very few purpose-made components. The quantitative determination of tobramycin required 20 s for a reading. The calibration curve for the range from 50 to 200 μM was found to be linear with a correlation coefficient of r = 0.9994. A good reproducibility was obtained (3% relative standard deviation) and the limit of detection was determined as 8 μM. As the concentration of the active ingredient in the eye drops (ophthalmic solutions) is too high for the sensitivity of the instrument, the samples had to be diluted appropriately. GRAPHICAL ABSTRACT: [Image: see text] Springer Berlin Heidelberg 2022-04-05 2022 /pmc/articles/PMC9124657/ /pubmed/35381854 http://dx.doi.org/10.1007/s00216-022-04050-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Paper
Chantipmanee, Nattapong
Hauser, Peter C.
Determination of tobramycin in eye drops with an open-source hardware ion mobility spectrometer
title Determination of tobramycin in eye drops with an open-source hardware ion mobility spectrometer
title_full Determination of tobramycin in eye drops with an open-source hardware ion mobility spectrometer
title_fullStr Determination of tobramycin in eye drops with an open-source hardware ion mobility spectrometer
title_full_unstemmed Determination of tobramycin in eye drops with an open-source hardware ion mobility spectrometer
title_short Determination of tobramycin in eye drops with an open-source hardware ion mobility spectrometer
title_sort determination of tobramycin in eye drops with an open-source hardware ion mobility spectrometer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124657/
https://www.ncbi.nlm.nih.gov/pubmed/35381854
http://dx.doi.org/10.1007/s00216-022-04050-2
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