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Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging
Perivascular fibroblasts (PVFs) are recognized for their pro-fibrotic role in many central nervous system disorders. Like mural cells, PVFs surround blood vessels and express Pdgfrβ. However, these shared attributes hinder the ability to distinguish PVFs from mural cells. We used in vivo two-photon...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9125487/ https://www.ncbi.nlm.nih.gov/pubmed/34929105 http://dx.doi.org/10.1177/0271678X211068528 |
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author | Bonney, Stephanie K Sullivan, Liam T Cherry, Timothy J Daneman, Richard Shih, Andy Y |
author_facet | Bonney, Stephanie K Sullivan, Liam T Cherry, Timothy J Daneman, Richard Shih, Andy Y |
author_sort | Bonney, Stephanie K |
collection | PubMed |
description | Perivascular fibroblasts (PVFs) are recognized for their pro-fibrotic role in many central nervous system disorders. Like mural cells, PVFs surround blood vessels and express Pdgfrβ. However, these shared attributes hinder the ability to distinguish PVFs from mural cells. We used in vivo two-photon imaging and transgenic mice with PVF-targeting promoters (Col1a1 or Col1a2) to compare the structure and distribution of PVFs and mural cells in cerebral cortex of healthy, adult mice. We show that PVFs localize to all cortical penetrating arterioles and their offshoots (arteriole-capillary transition zone), as well as the main trunk of only larger ascending venules. However, the capillary zone is devoid of PVF coverage. PVFs display short-range mobility along the vessel wall and exhibit distinct structural features (flattened somata and thin ruffled processes) not seen with smooth muscle cells or pericytes. These findings clarify that PVFs and mural cells are distinct cell types coexisting in a similar perivascular niche. |
format | Online Article Text |
id | pubmed-9125487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-91254872022-05-24 Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging Bonney, Stephanie K Sullivan, Liam T Cherry, Timothy J Daneman, Richard Shih, Andy Y J Cereb Blood Flow Metab Original Articles Perivascular fibroblasts (PVFs) are recognized for their pro-fibrotic role in many central nervous system disorders. Like mural cells, PVFs surround blood vessels and express Pdgfrβ. However, these shared attributes hinder the ability to distinguish PVFs from mural cells. We used in vivo two-photon imaging and transgenic mice with PVF-targeting promoters (Col1a1 or Col1a2) to compare the structure and distribution of PVFs and mural cells in cerebral cortex of healthy, adult mice. We show that PVFs localize to all cortical penetrating arterioles and their offshoots (arteriole-capillary transition zone), as well as the main trunk of only larger ascending venules. However, the capillary zone is devoid of PVF coverage. PVFs display short-range mobility along the vessel wall and exhibit distinct structural features (flattened somata and thin ruffled processes) not seen with smooth muscle cells or pericytes. These findings clarify that PVFs and mural cells are distinct cell types coexisting in a similar perivascular niche. SAGE Publications 2021-12-20 2022-06 /pmc/articles/PMC9125487/ /pubmed/34929105 http://dx.doi.org/10.1177/0271678X211068528 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Articles Bonney, Stephanie K Sullivan, Liam T Cherry, Timothy J Daneman, Richard Shih, Andy Y Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging |
title | Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging |
title_full | Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging |
title_fullStr | Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging |
title_full_unstemmed | Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging |
title_short | Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging |
title_sort | distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9125487/ https://www.ncbi.nlm.nih.gov/pubmed/34929105 http://dx.doi.org/10.1177/0271678X211068528 |
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