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In vitro anti-influenza assessment of anionic compounds ascorbate, acetate and citrate

BACKGROUND: Influenza A virus (IAV) infection remains a serious public health threat. Due to drug resistance and side effects of the conventional antiviral drugs, repurposing the available natural compounds with high tolerability and fewer side effects has attracted researchers’ attention. The aim o...

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Autores principales: Shokouhi Targhi, Hadiseh, Mehrbod, Parvaneh, Fotouhi, Fatemeh, Amininasab, Mehriar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9125540/
https://www.ncbi.nlm.nih.gov/pubmed/35606770
http://dx.doi.org/10.1186/s12985-022-01823-0
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author Shokouhi Targhi, Hadiseh
Mehrbod, Parvaneh
Fotouhi, Fatemeh
Amininasab, Mehriar
author_facet Shokouhi Targhi, Hadiseh
Mehrbod, Parvaneh
Fotouhi, Fatemeh
Amininasab, Mehriar
author_sort Shokouhi Targhi, Hadiseh
collection PubMed
description BACKGROUND: Influenza A virus (IAV) infection remains a serious public health threat. Due to drug resistance and side effects of the conventional antiviral drugs, repurposing the available natural compounds with high tolerability and fewer side effects has attracted researchers’ attention. The aim of this study was to screen in vitro anti-influenza activity of three anionic compounds ascorbate, acetate, and citrate. METHODS: The non-cytotoxic concentration of the compounds was determined by MTT assay and examined for the activity against IAV in simultaneous, pre-, and post-penetration combination treatments over 1 h incubation on Madin-Darby Canine Kidney (MDCK) cell line. The virus titer and viral load were determined using hemagglutination assay (HA) and qPCR, respectively. Few pro-inflammatory and anti-inflammatory cytokines were evaluated at RNA and protein levels by qPCR and ELISA, respectively. RESULTS: The non-cytotoxic concentrations of the ascorbate (200 mg/ml), acetate and citrate (both 3 mg/ml) reduced the viral titer by 6.5, 4.5, and 1.5 logs in the simultaneous combination treatment. The M protein gene copy number decreased significantly in simultaneous treatment (P < 0.01). The expression of cytokines was also affected by the treatment of these compounds. CONCLUSIONS: These anionic compounds could affect the influenza virus load, thereby reducing pro-inflammatory cytokines and increasing anti-inflammatory cytokines levels. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-022-01823-0.
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spelling pubmed-91255402022-05-23 In vitro anti-influenza assessment of anionic compounds ascorbate, acetate and citrate Shokouhi Targhi, Hadiseh Mehrbod, Parvaneh Fotouhi, Fatemeh Amininasab, Mehriar Virol J Research BACKGROUND: Influenza A virus (IAV) infection remains a serious public health threat. Due to drug resistance and side effects of the conventional antiviral drugs, repurposing the available natural compounds with high tolerability and fewer side effects has attracted researchers’ attention. The aim of this study was to screen in vitro anti-influenza activity of three anionic compounds ascorbate, acetate, and citrate. METHODS: The non-cytotoxic concentration of the compounds was determined by MTT assay and examined for the activity against IAV in simultaneous, pre-, and post-penetration combination treatments over 1 h incubation on Madin-Darby Canine Kidney (MDCK) cell line. The virus titer and viral load were determined using hemagglutination assay (HA) and qPCR, respectively. Few pro-inflammatory and anti-inflammatory cytokines were evaluated at RNA and protein levels by qPCR and ELISA, respectively. RESULTS: The non-cytotoxic concentrations of the ascorbate (200 mg/ml), acetate and citrate (both 3 mg/ml) reduced the viral titer by 6.5, 4.5, and 1.5 logs in the simultaneous combination treatment. The M protein gene copy number decreased significantly in simultaneous treatment (P < 0.01). The expression of cytokines was also affected by the treatment of these compounds. CONCLUSIONS: These anionic compounds could affect the influenza virus load, thereby reducing pro-inflammatory cytokines and increasing anti-inflammatory cytokines levels. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-022-01823-0. BioMed Central 2022-05-23 /pmc/articles/PMC9125540/ /pubmed/35606770 http://dx.doi.org/10.1186/s12985-022-01823-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Shokouhi Targhi, Hadiseh
Mehrbod, Parvaneh
Fotouhi, Fatemeh
Amininasab, Mehriar
In vitro anti-influenza assessment of anionic compounds ascorbate, acetate and citrate
title In vitro anti-influenza assessment of anionic compounds ascorbate, acetate and citrate
title_full In vitro anti-influenza assessment of anionic compounds ascorbate, acetate and citrate
title_fullStr In vitro anti-influenza assessment of anionic compounds ascorbate, acetate and citrate
title_full_unstemmed In vitro anti-influenza assessment of anionic compounds ascorbate, acetate and citrate
title_short In vitro anti-influenza assessment of anionic compounds ascorbate, acetate and citrate
title_sort in vitro anti-influenza assessment of anionic compounds ascorbate, acetate and citrate
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9125540/
https://www.ncbi.nlm.nih.gov/pubmed/35606770
http://dx.doi.org/10.1186/s12985-022-01823-0
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