The effect of the probiotic consortia on SARS-CoV-2 infection in ferrets and on human immune cell response invitro

Probiotics have been suggested as one solution to counter detrimental health effects by SARS-CoV-2; however, data so far is scarce. We tested the effect of two probiotic consortia, OL-1 and OL-2, against SARS-CoV-2 in ferrets and assessed their effect on cytokine production and transcriptome in a hu...

Descripción completa

Detalles Bibliográficos
Autores principales: Lehtinen, Markus J., Kumar, Ritesh, Zabel, Bryan, Mäkelä, Sanna M., Nedveck, Derek, Tang, Peipei, Latvala, Sinikka, Guery, Sebastien, Budinoff, Charles R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9125999/
https://www.ncbi.nlm.nih.gov/pubmed/35634575
http://dx.doi.org/10.1016/j.isci.2022.104445
_version_ 1784712048899260416
author Lehtinen, Markus J.
Kumar, Ritesh
Zabel, Bryan
Mäkelä, Sanna M.
Nedveck, Derek
Tang, Peipei
Latvala, Sinikka
Guery, Sebastien
Budinoff, Charles R.
author_facet Lehtinen, Markus J.
Kumar, Ritesh
Zabel, Bryan
Mäkelä, Sanna M.
Nedveck, Derek
Tang, Peipei
Latvala, Sinikka
Guery, Sebastien
Budinoff, Charles R.
author_sort Lehtinen, Markus J.
collection PubMed
description Probiotics have been suggested as one solution to counter detrimental health effects by SARS-CoV-2; however, data so far is scarce. We tested the effect of two probiotic consortia, OL-1 and OL-2, against SARS-CoV-2 in ferrets and assessed their effect on cytokine production and transcriptome in a human monocyte-derived macrophage (Mf) and dendritic cell (DC) model. The results showed that the consortia significantly reduced the viral load, modulated immune response, and regulated viral receptor expression in ferrets compared to placebo. In the human Mf and DC model, OL-1 and OL-2-induced cytokine production and genes related to SARS-CoV-2 antiviral immunity. The study results indicate that probiotic stimulation of the ferret immune system leads to improved antiviral immunity against SARS-COV-2, and the genes and cytokines associated with anti-SARS-CoV-2 immunity are stimulated in human immune cells in vitro. The effect of the consortia against SARS-CoV-2 warrants further investigations in human clinical trials.
format Online
Article
Text
id pubmed-9125999
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-91259992022-05-23 The effect of the probiotic consortia on SARS-CoV-2 infection in ferrets and on human immune cell response invitro Lehtinen, Markus J. Kumar, Ritesh Zabel, Bryan Mäkelä, Sanna M. Nedveck, Derek Tang, Peipei Latvala, Sinikka Guery, Sebastien Budinoff, Charles R. iScience Article Probiotics have been suggested as one solution to counter detrimental health effects by SARS-CoV-2; however, data so far is scarce. We tested the effect of two probiotic consortia, OL-1 and OL-2, against SARS-CoV-2 in ferrets and assessed their effect on cytokine production and transcriptome in a human monocyte-derived macrophage (Mf) and dendritic cell (DC) model. The results showed that the consortia significantly reduced the viral load, modulated immune response, and regulated viral receptor expression in ferrets compared to placebo. In the human Mf and DC model, OL-1 and OL-2-induced cytokine production and genes related to SARS-CoV-2 antiviral immunity. The study results indicate that probiotic stimulation of the ferret immune system leads to improved antiviral immunity against SARS-COV-2, and the genes and cytokines associated with anti-SARS-CoV-2 immunity are stimulated in human immune cells in vitro. The effect of the consortia against SARS-CoV-2 warrants further investigations in human clinical trials. Elsevier 2022-05-23 /pmc/articles/PMC9125999/ /pubmed/35634575 http://dx.doi.org/10.1016/j.isci.2022.104445 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Lehtinen, Markus J.
Kumar, Ritesh
Zabel, Bryan
Mäkelä, Sanna M.
Nedveck, Derek
Tang, Peipei
Latvala, Sinikka
Guery, Sebastien
Budinoff, Charles R.
The effect of the probiotic consortia on SARS-CoV-2 infection in ferrets and on human immune cell response invitro
title The effect of the probiotic consortia on SARS-CoV-2 infection in ferrets and on human immune cell response invitro
title_full The effect of the probiotic consortia on SARS-CoV-2 infection in ferrets and on human immune cell response invitro
title_fullStr The effect of the probiotic consortia on SARS-CoV-2 infection in ferrets and on human immune cell response invitro
title_full_unstemmed The effect of the probiotic consortia on SARS-CoV-2 infection in ferrets and on human immune cell response invitro
title_short The effect of the probiotic consortia on SARS-CoV-2 infection in ferrets and on human immune cell response invitro
title_sort effect of the probiotic consortia on sars-cov-2 infection in ferrets and on human immune cell response invitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9125999/
https://www.ncbi.nlm.nih.gov/pubmed/35634575
http://dx.doi.org/10.1016/j.isci.2022.104445
work_keys_str_mv AT lehtinenmarkusj theeffectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro
AT kumarritesh theeffectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro
AT zabelbryan theeffectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro
AT makelasannam theeffectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro
AT nedveckderek theeffectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro
AT tangpeipei theeffectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro
AT latvalasinikka theeffectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro
AT guerysebastien theeffectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro
AT budinoffcharlesr theeffectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro
AT lehtinenmarkusj effectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro
AT kumarritesh effectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro
AT zabelbryan effectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro
AT makelasannam effectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro
AT nedveckderek effectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro
AT tangpeipei effectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro
AT latvalasinikka effectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro
AT guerysebastien effectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro
AT budinoffcharlesr effectoftheprobioticconsortiaonsarscov2infectioninferretsandonhumanimmunecellresponseinvitro

Ejemplares similares