Citric acid-mediated green synthesis of selenium nanoparticles: antioxidant, antimicrobial, and anticoagulant potential applications

Using microwave technique in the presence of citric acid, selenium nanoparticles (SeNPs) were fabricated. The morphological characteristics revealed that the spherical SeNPs with diameters ranging from 10.5 to 20 nm aggregated spherical shapes with sizes ranging from 0.67 to 0.83 mm. Moreover, the antioxidant efficacy was assessed by the DPPH radical scavenging test, which depicted that green-prepared nanoparticle at a 106.3 mg/mL dosage had the maximum scavenging capacity (301.1 ± 11.42 mg/g). Otherwise, with nanoparticle concentrations of 500 mg/ml, in vitro cell viability of SeNPs through human breast cancer MCF-7 cell lines was reduced to 61.2 ± 2.2% after 1 day of exposure. The antibacterial activity was tested against G-negative Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E. coli), G-positive bacteria Bacillus subtilis (B. subtilis), and Staphylococcus aureus (S. aureus), which demonstrated that SeNPs had little activity against S. aureus. Still, it had the highest activity against E. coli, with a zone of inhibition (ZOI) of 25.2 ± 1.5 mm compared to 16.0 ± 0.6 mm for the standard antibiotic. Most notably, biogenic SeNPs have anticoagulant activities using activated partial thromboplastin time (aPTT) assessment. Based on previous findings, SeNPs can be used in medical aid and their cell viability, antioxidant, anticoagulant, and effects on bacteria.