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Exploring Endothelial Colony-Forming Cells to Better Understand the Pathophysiology of Disease: An Updated Review

Endothelial cell (EC) dysfunction has been implicated in a variety of pathological conditions. The collection of ECs from patients is typically conducted postmortem or through invasive procedures, such as surgery and interventional procedures, hampering efforts to clarify the role of ECs in disease...

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Detalles Bibliográficos
Autores principales: Zhang, Qiuwang, Cannavicci, Anthony, Kutryk, Michael J. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9126670/
https://www.ncbi.nlm.nih.gov/pubmed/35615696
http://dx.doi.org/10.1155/2022/4460041
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author Zhang, Qiuwang
Cannavicci, Anthony
Kutryk, Michael J. B.
author_facet Zhang, Qiuwang
Cannavicci, Anthony
Kutryk, Michael J. B.
author_sort Zhang, Qiuwang
collection PubMed
description Endothelial cell (EC) dysfunction has been implicated in a variety of pathological conditions. The collection of ECs from patients is typically conducted postmortem or through invasive procedures, such as surgery and interventional procedures, hampering efforts to clarify the role of ECs in disease onset and progression. In contrast, endothelial colony-forming cells (ECFCs), also termed late endothelial progenitor cells, late outgrowth endothelial cells, blood outgrowth endothelial cells, or endothelial outgrowth cells, are obtained in a minimally invasive manner, namely, by the culture of human peripheral blood mononuclear cells in endothelial growth medium. ECFCs resemble mature ECs phenotypically, genetically, and functionally, making them excellent surrogates for ECs. Numerous studies have been performed that examined ECFC function in conditions such as coronary artery disease, diabetes mellitus, hereditary hemorrhagic telangiectasia, congenital bicuspid aortic valve disease, pulmonary arterial hypertension, venous thromboembolic disease, and von Willebrand disease. Here, we provide an updated review of studies using ECFCs that were performed to better understand the pathophysiology of disease. We also discuss the potential of ECFCs as disease biomarkers and the standardized methods to culture, quantify, and evaluate ECFCs and suggest the future direction of research in this field.
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spelling pubmed-91266702022-05-24 Exploring Endothelial Colony-Forming Cells to Better Understand the Pathophysiology of Disease: An Updated Review Zhang, Qiuwang Cannavicci, Anthony Kutryk, Michael J. B. Stem Cells Int Review Article Endothelial cell (EC) dysfunction has been implicated in a variety of pathological conditions. The collection of ECs from patients is typically conducted postmortem or through invasive procedures, such as surgery and interventional procedures, hampering efforts to clarify the role of ECs in disease onset and progression. In contrast, endothelial colony-forming cells (ECFCs), also termed late endothelial progenitor cells, late outgrowth endothelial cells, blood outgrowth endothelial cells, or endothelial outgrowth cells, are obtained in a minimally invasive manner, namely, by the culture of human peripheral blood mononuclear cells in endothelial growth medium. ECFCs resemble mature ECs phenotypically, genetically, and functionally, making them excellent surrogates for ECs. Numerous studies have been performed that examined ECFC function in conditions such as coronary artery disease, diabetes mellitus, hereditary hemorrhagic telangiectasia, congenital bicuspid aortic valve disease, pulmonary arterial hypertension, venous thromboembolic disease, and von Willebrand disease. Here, we provide an updated review of studies using ECFCs that were performed to better understand the pathophysiology of disease. We also discuss the potential of ECFCs as disease biomarkers and the standardized methods to culture, quantify, and evaluate ECFCs and suggest the future direction of research in this field. Hindawi 2022-05-16 /pmc/articles/PMC9126670/ /pubmed/35615696 http://dx.doi.org/10.1155/2022/4460041 Text en Copyright © 2022 Qiuwang Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Zhang, Qiuwang
Cannavicci, Anthony
Kutryk, Michael J. B.
Exploring Endothelial Colony-Forming Cells to Better Understand the Pathophysiology of Disease: An Updated Review
title Exploring Endothelial Colony-Forming Cells to Better Understand the Pathophysiology of Disease: An Updated Review
title_full Exploring Endothelial Colony-Forming Cells to Better Understand the Pathophysiology of Disease: An Updated Review
title_fullStr Exploring Endothelial Colony-Forming Cells to Better Understand the Pathophysiology of Disease: An Updated Review
title_full_unstemmed Exploring Endothelial Colony-Forming Cells to Better Understand the Pathophysiology of Disease: An Updated Review
title_short Exploring Endothelial Colony-Forming Cells to Better Understand the Pathophysiology of Disease: An Updated Review
title_sort exploring endothelial colony-forming cells to better understand the pathophysiology of disease: an updated review
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9126670/
https://www.ncbi.nlm.nih.gov/pubmed/35615696
http://dx.doi.org/10.1155/2022/4460041
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