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Generation of AAVS1 integrated doxycycline-inducible CRISPR-Prime Editor human induced pluripotent stem cell line

Prime editing uses the Cas9 nickase fused to a reverse transcriptase to copy a DNA sequence into a specific locus from a ‘prime editing’ guide RNA (pegRNA), eliminating the need for double-stranded DNA breaks and donor DNA templates. To facilitate prime editing in human induced pluripotent stem cell...

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Detalles Bibliográficos
Autores principales:	Bharucha, Nike, Ataam, Jennifer Arthur, Gavidia, Alexandra A., Karakikes, Ioannis
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9126997/ https://www.ncbi.nlm.nih.gov/pubmed/34875545 http://dx.doi.org/10.1016/j.scr.2021.102610

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