Protocol for characterizing strigolactones released by plant roots

The plant hormone strigolactones (SLs) are secreted by plant roots to act as rhizospheric signals. Here, we present a protocol for characterizing plant-released SLs. We first outline all necessary steps required for collection, processing, and analysis of plant root exudates using the C(18) column f...

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Detalles Bibliográficos
Autores principales: Wang, Jian You, Chen, Guan-Ting Erica, Jamil, Muhammad, Braguy, Justine, Sioud, Salim, Liew, Kit Xi, Balakrishna, Aparna, Al-Babili, Salim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127222/
https://www.ncbi.nlm.nih.gov/pubmed/35620066
http://dx.doi.org/10.1016/j.xpro.2022.101352
Descripción
Sumario:The plant hormone strigolactones (SLs) are secreted by plant roots to act as rhizospheric signals. Here, we present a protocol for characterizing plant-released SLs. We first outline all necessary steps required for collection, processing, and analysis of plant root exudates using the C(18) column for SL extraction, followed by liquid chromatography-mass spectrometry (LC-MS) for SL quantification. We then describe image processing by SeedQuant, an open-source artificial-intelligence-based software, for measuring the biological activity of SLs in inducing root parasitic plant seed germination. For complete details on the use and execution of this protocol, please refer to Wang et al. (2019) and Braguy et al. (2021).

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