Cargando…

Sprayable biofilm – Agarose hydrogels as 3D matrix for enhanced productivity in bioelectrochemical systems

Bio-based energy production utilizing renewable resources can be realized by exoelectrogenic organisms and their application in bioelectrochemical systems (BES). These organisms catalyze the direct conversion of chemical into electrical energy and are already widely used in bioelectronics and biosen...

Descripción completa

Detalles Bibliográficos
Autores principales: Knoll, Melanie Tabea, Fuderer, Emely, Gescher, Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127277/
https://www.ncbi.nlm.nih.gov/pubmed/35619831
http://dx.doi.org/10.1016/j.bioflm.2022.100077
_version_ 1784712315771289600
author Knoll, Melanie Tabea
Fuderer, Emely
Gescher, Johannes
author_facet Knoll, Melanie Tabea
Fuderer, Emely
Gescher, Johannes
author_sort Knoll, Melanie Tabea
collection PubMed
description Bio-based energy production utilizing renewable resources can be realized by exoelectrogenic organisms and their application in bioelectrochemical systems (BES). These organisms catalyze the direct conversion of chemical into electrical energy and are already widely used in bioelectronics and biosensing. However, the biofilm-electrode interaction is a factor that limits sufficient space-time-yields for industrial applications. In this study, a hydrogel matrix consisting of agarose fibers was utilized as a scaffold for S. oneidensis cells to improve anodic processes in BES. This synthetic, scalable biofilm reached a higher current density in BES in comparison to naturally formed biofilms. Complemented with carbon nanofibers and riboflavin, the application of this functionalized hydrogel containing S. oneidensis cells led to an overall 9.1-fold increase in current density to 1324 mA m(−2) in comparison to 145 mA m(−2) for the planktonic control. In addition, the synthetic biofilm can be applied by spraying onto surfaces using a novel spray applicator. The latter allows to apply the biofilm effortless on large surfaces which will facilitate scalability and thus industrial application.
format Online
Article
Text
id pubmed-9127277
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-91272772022-05-25 Sprayable biofilm – Agarose hydrogels as 3D matrix for enhanced productivity in bioelectrochemical systems Knoll, Melanie Tabea Fuderer, Emely Gescher, Johannes Biofilm Article Bio-based energy production utilizing renewable resources can be realized by exoelectrogenic organisms and their application in bioelectrochemical systems (BES). These organisms catalyze the direct conversion of chemical into electrical energy and are already widely used in bioelectronics and biosensing. However, the biofilm-electrode interaction is a factor that limits sufficient space-time-yields for industrial applications. In this study, a hydrogel matrix consisting of agarose fibers was utilized as a scaffold for S. oneidensis cells to improve anodic processes in BES. This synthetic, scalable biofilm reached a higher current density in BES in comparison to naturally formed biofilms. Complemented with carbon nanofibers and riboflavin, the application of this functionalized hydrogel containing S. oneidensis cells led to an overall 9.1-fold increase in current density to 1324 mA m(−2) in comparison to 145 mA m(−2) for the planktonic control. In addition, the synthetic biofilm can be applied by spraying onto surfaces using a novel spray applicator. The latter allows to apply the biofilm effortless on large surfaces which will facilitate scalability and thus industrial application. Elsevier 2022-05-18 /pmc/articles/PMC9127277/ /pubmed/35619831 http://dx.doi.org/10.1016/j.bioflm.2022.100077 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Knoll, Melanie Tabea
Fuderer, Emely
Gescher, Johannes
Sprayable biofilm – Agarose hydrogels as 3D matrix for enhanced productivity in bioelectrochemical systems
title Sprayable biofilm – Agarose hydrogels as 3D matrix for enhanced productivity in bioelectrochemical systems
title_full Sprayable biofilm – Agarose hydrogels as 3D matrix for enhanced productivity in bioelectrochemical systems
title_fullStr Sprayable biofilm – Agarose hydrogels as 3D matrix for enhanced productivity in bioelectrochemical systems
title_full_unstemmed Sprayable biofilm – Agarose hydrogels as 3D matrix for enhanced productivity in bioelectrochemical systems
title_short Sprayable biofilm – Agarose hydrogels as 3D matrix for enhanced productivity in bioelectrochemical systems
title_sort sprayable biofilm – agarose hydrogels as 3d matrix for enhanced productivity in bioelectrochemical systems
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127277/
https://www.ncbi.nlm.nih.gov/pubmed/35619831
http://dx.doi.org/10.1016/j.bioflm.2022.100077
work_keys_str_mv AT knollmelanietabea sprayablebiofilmagarosehydrogelsas3dmatrixforenhancedproductivityinbioelectrochemicalsystems
AT fudereremely sprayablebiofilmagarosehydrogelsas3dmatrixforenhancedproductivityinbioelectrochemicalsystems
AT gescherjohannes sprayablebiofilmagarosehydrogelsas3dmatrixforenhancedproductivityinbioelectrochemicalsystems