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Quantitative Determination of Whey Protein to Casein Ratio in Infant Formula Milk Powder

This study was aimed to establish a method for quantitatively determining the ratio of whey protein in the total protein of infant formula by respectively selecting two characteristic peptides from whey protein and casein and calculating the ratio between the characteristic peptides. A nanoliter hig...

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Autores principales: Xu, Tao, Chen, Jingyao, Yang, Kai, Qiao, Weicang, Zhao, Junying, Chen, Lijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127462/
https://www.ncbi.nlm.nih.gov/pubmed/35620655
http://dx.doi.org/10.3389/fchem.2022.872251
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author Xu, Tao
Chen, Jingyao
Yang, Kai
Qiao, Weicang
Zhao, Junying
Chen, Lijun
author_facet Xu, Tao
Chen, Jingyao
Yang, Kai
Qiao, Weicang
Zhao, Junying
Chen, Lijun
author_sort Xu, Tao
collection PubMed
description This study was aimed to establish a method for quantitatively determining the ratio of whey protein in the total protein of infant formula by respectively selecting two characteristic peptides from whey protein and casein and calculating the ratio between the characteristic peptides. A nanoliter high-performance liquid chromatography tandem high-resolution mass spectrometry (Q Exactive) was used to simultaneously detect the characteristic peptides of two main whey proteins and two main caseins. The characteristic peptides were calculated, predicted, and screened using the ExPASy website, and peptide information was confirmed by database retrieval after the analysis by using a high-resolution mass spectrometer. The matrix effect was compensated by comparing the characteristic peptides in whey protein with those in casein protein, in which isotope internal standards were not required. The influence of the changes of the protein content in whey protein and casein on the detection method was eliminated by the calculation formula designed by ourselves. In this detection method, the sample was stable in the total protein concentration range of between 0.1 and 0.4 mg/ml. In the simulated industrial processing environment, with desalted whey powder, the recovery rate was 98.63–113.33% under different spiked levels with good reproducibility (RSD<8%). The RSDs of intraday and interday precisions were 2.03–9.35% and 0.61–11.02%, respectively. The different processing procedures of samples had no significant impact on the detection of whey protein (RSD% for milk samples treated by different processing techniques was 2.97%). The quantitation method of whey protein was applied to evaluate the whey protein content in different brands of commercially available milk powder. In summary, the proposed method was applicable for quantitative analysis of whey proteins in the infant formula.
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spelling pubmed-91274622022-05-25 Quantitative Determination of Whey Protein to Casein Ratio in Infant Formula Milk Powder Xu, Tao Chen, Jingyao Yang, Kai Qiao, Weicang Zhao, Junying Chen, Lijun Front Chem Chemistry This study was aimed to establish a method for quantitatively determining the ratio of whey protein in the total protein of infant formula by respectively selecting two characteristic peptides from whey protein and casein and calculating the ratio between the characteristic peptides. A nanoliter high-performance liquid chromatography tandem high-resolution mass spectrometry (Q Exactive) was used to simultaneously detect the characteristic peptides of two main whey proteins and two main caseins. The characteristic peptides were calculated, predicted, and screened using the ExPASy website, and peptide information was confirmed by database retrieval after the analysis by using a high-resolution mass spectrometer. The matrix effect was compensated by comparing the characteristic peptides in whey protein with those in casein protein, in which isotope internal standards were not required. The influence of the changes of the protein content in whey protein and casein on the detection method was eliminated by the calculation formula designed by ourselves. In this detection method, the sample was stable in the total protein concentration range of between 0.1 and 0.4 mg/ml. In the simulated industrial processing environment, with desalted whey powder, the recovery rate was 98.63–113.33% under different spiked levels with good reproducibility (RSD<8%). The RSDs of intraday and interday precisions were 2.03–9.35% and 0.61–11.02%, respectively. The different processing procedures of samples had no significant impact on the detection of whey protein (RSD% for milk samples treated by different processing techniques was 2.97%). The quantitation method of whey protein was applied to evaluate the whey protein content in different brands of commercially available milk powder. In summary, the proposed method was applicable for quantitative analysis of whey proteins in the infant formula. Frontiers Media S.A. 2022-05-10 /pmc/articles/PMC9127462/ /pubmed/35620655 http://dx.doi.org/10.3389/fchem.2022.872251 Text en Copyright © 2022 Xu, Chen, Yang, Qiao, Zhao and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Xu, Tao
Chen, Jingyao
Yang, Kai
Qiao, Weicang
Zhao, Junying
Chen, Lijun
Quantitative Determination of Whey Protein to Casein Ratio in Infant Formula Milk Powder
title Quantitative Determination of Whey Protein to Casein Ratio in Infant Formula Milk Powder
title_full Quantitative Determination of Whey Protein to Casein Ratio in Infant Formula Milk Powder
title_fullStr Quantitative Determination of Whey Protein to Casein Ratio in Infant Formula Milk Powder
title_full_unstemmed Quantitative Determination of Whey Protein to Casein Ratio in Infant Formula Milk Powder
title_short Quantitative Determination of Whey Protein to Casein Ratio in Infant Formula Milk Powder
title_sort quantitative determination of whey protein to casein ratio in infant formula milk powder
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127462/
https://www.ncbi.nlm.nih.gov/pubmed/35620655
http://dx.doi.org/10.3389/fchem.2022.872251
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