Cargando…
Quantitative Determination of Whey Protein to Casein Ratio in Infant Formula Milk Powder
This study was aimed to establish a method for quantitatively determining the ratio of whey protein in the total protein of infant formula by respectively selecting two characteristic peptides from whey protein and casein and calculating the ratio between the characteristic peptides. A nanoliter hig...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127462/ https://www.ncbi.nlm.nih.gov/pubmed/35620655 http://dx.doi.org/10.3389/fchem.2022.872251 |
_version_ | 1784712357398708224 |
---|---|
author | Xu, Tao Chen, Jingyao Yang, Kai Qiao, Weicang Zhao, Junying Chen, Lijun |
author_facet | Xu, Tao Chen, Jingyao Yang, Kai Qiao, Weicang Zhao, Junying Chen, Lijun |
author_sort | Xu, Tao |
collection | PubMed |
description | This study was aimed to establish a method for quantitatively determining the ratio of whey protein in the total protein of infant formula by respectively selecting two characteristic peptides from whey protein and casein and calculating the ratio between the characteristic peptides. A nanoliter high-performance liquid chromatography tandem high-resolution mass spectrometry (Q Exactive) was used to simultaneously detect the characteristic peptides of two main whey proteins and two main caseins. The characteristic peptides were calculated, predicted, and screened using the ExPASy website, and peptide information was confirmed by database retrieval after the analysis by using a high-resolution mass spectrometer. The matrix effect was compensated by comparing the characteristic peptides in whey protein with those in casein protein, in which isotope internal standards were not required. The influence of the changes of the protein content in whey protein and casein on the detection method was eliminated by the calculation formula designed by ourselves. In this detection method, the sample was stable in the total protein concentration range of between 0.1 and 0.4 mg/ml. In the simulated industrial processing environment, with desalted whey powder, the recovery rate was 98.63–113.33% under different spiked levels with good reproducibility (RSD<8%). The RSDs of intraday and interday precisions were 2.03–9.35% and 0.61–11.02%, respectively. The different processing procedures of samples had no significant impact on the detection of whey protein (RSD% for milk samples treated by different processing techniques was 2.97%). The quantitation method of whey protein was applied to evaluate the whey protein content in different brands of commercially available milk powder. In summary, the proposed method was applicable for quantitative analysis of whey proteins in the infant formula. |
format | Online Article Text |
id | pubmed-9127462 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91274622022-05-25 Quantitative Determination of Whey Protein to Casein Ratio in Infant Formula Milk Powder Xu, Tao Chen, Jingyao Yang, Kai Qiao, Weicang Zhao, Junying Chen, Lijun Front Chem Chemistry This study was aimed to establish a method for quantitatively determining the ratio of whey protein in the total protein of infant formula by respectively selecting two characteristic peptides from whey protein and casein and calculating the ratio between the characteristic peptides. A nanoliter high-performance liquid chromatography tandem high-resolution mass spectrometry (Q Exactive) was used to simultaneously detect the characteristic peptides of two main whey proteins and two main caseins. The characteristic peptides were calculated, predicted, and screened using the ExPASy website, and peptide information was confirmed by database retrieval after the analysis by using a high-resolution mass spectrometer. The matrix effect was compensated by comparing the characteristic peptides in whey protein with those in casein protein, in which isotope internal standards were not required. The influence of the changes of the protein content in whey protein and casein on the detection method was eliminated by the calculation formula designed by ourselves. In this detection method, the sample was stable in the total protein concentration range of between 0.1 and 0.4 mg/ml. In the simulated industrial processing environment, with desalted whey powder, the recovery rate was 98.63–113.33% under different spiked levels with good reproducibility (RSD<8%). The RSDs of intraday and interday precisions were 2.03–9.35% and 0.61–11.02%, respectively. The different processing procedures of samples had no significant impact on the detection of whey protein (RSD% for milk samples treated by different processing techniques was 2.97%). The quantitation method of whey protein was applied to evaluate the whey protein content in different brands of commercially available milk powder. In summary, the proposed method was applicable for quantitative analysis of whey proteins in the infant formula. Frontiers Media S.A. 2022-05-10 /pmc/articles/PMC9127462/ /pubmed/35620655 http://dx.doi.org/10.3389/fchem.2022.872251 Text en Copyright © 2022 Xu, Chen, Yang, Qiao, Zhao and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Xu, Tao Chen, Jingyao Yang, Kai Qiao, Weicang Zhao, Junying Chen, Lijun Quantitative Determination of Whey Protein to Casein Ratio in Infant Formula Milk Powder |
title | Quantitative Determination of Whey Protein to Casein Ratio in Infant Formula Milk Powder |
title_full | Quantitative Determination of Whey Protein to Casein Ratio in Infant Formula Milk Powder |
title_fullStr | Quantitative Determination of Whey Protein to Casein Ratio in Infant Formula Milk Powder |
title_full_unstemmed | Quantitative Determination of Whey Protein to Casein Ratio in Infant Formula Milk Powder |
title_short | Quantitative Determination of Whey Protein to Casein Ratio in Infant Formula Milk Powder |
title_sort | quantitative determination of whey protein to casein ratio in infant formula milk powder |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127462/ https://www.ncbi.nlm.nih.gov/pubmed/35620655 http://dx.doi.org/10.3389/fchem.2022.872251 |
work_keys_str_mv | AT xutao quantitativedeterminationofwheyproteintocaseinratioininfantformulamilkpowder AT chenjingyao quantitativedeterminationofwheyproteintocaseinratioininfantformulamilkpowder AT yangkai quantitativedeterminationofwheyproteintocaseinratioininfantformulamilkpowder AT qiaoweicang quantitativedeterminationofwheyproteintocaseinratioininfantformulamilkpowder AT zhaojunying quantitativedeterminationofwheyproteintocaseinratioininfantformulamilkpowder AT chenlijun quantitativedeterminationofwheyproteintocaseinratioininfantformulamilkpowder |