Hybrid Sequencing Resolved Inverted Terminal Repeats in the Genome of Megavirus Baoshan

Mimivirus is a group of amoeba-infecting DNA viruses with linear double-strand genome. It is found to be ubiquitous in nature worldwide. Here, we reported the complete genome of a new member of Mimivirus lineage C isolated from a fresh water pond in Shanghai, China. Its 1,224,839-bp genome encoded 1...

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Autores principales: Xia, Yucheng, Cheng, Huanyu, Zhong, Jiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127612/
https://www.ncbi.nlm.nih.gov/pubmed/35620092
http://dx.doi.org/10.3389/fmicb.2022.831659
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author Xia, Yucheng
Cheng, Huanyu
Zhong, Jiang
author_facet Xia, Yucheng
Cheng, Huanyu
Zhong, Jiang
author_sort Xia, Yucheng
collection PubMed
description Mimivirus is a group of amoeba-infecting DNA viruses with linear double-strand genome. It is found to be ubiquitous in nature worldwide. Here, we reported the complete genome of a new member of Mimivirus lineage C isolated from a fresh water pond in Shanghai, China. Its 1,224,839-bp genome encoded 1,062 predicted ORFs. Combining the results of Nanopore, Illumina, and Sanger sequencing technologies, two identical 23,919 bp inverted terminal repeats (ITRs) were identified at both extremities of the viral linear genome, one of which was missing in the draft assembly based on Illumina data only. The discovery of ITRs of Mimivirus provided a new insight into Mimivirus genome structure.
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spelling pubmed-91276122022-05-25 Hybrid Sequencing Resolved Inverted Terminal Repeats in the Genome of Megavirus Baoshan Xia, Yucheng Cheng, Huanyu Zhong, Jiang Front Microbiol Microbiology Mimivirus is a group of amoeba-infecting DNA viruses with linear double-strand genome. It is found to be ubiquitous in nature worldwide. Here, we reported the complete genome of a new member of Mimivirus lineage C isolated from a fresh water pond in Shanghai, China. Its 1,224,839-bp genome encoded 1,062 predicted ORFs. Combining the results of Nanopore, Illumina, and Sanger sequencing technologies, two identical 23,919 bp inverted terminal repeats (ITRs) were identified at both extremities of the viral linear genome, one of which was missing in the draft assembly based on Illumina data only. The discovery of ITRs of Mimivirus provided a new insight into Mimivirus genome structure. Frontiers Media S.A. 2022-05-10 /pmc/articles/PMC9127612/ /pubmed/35620092 http://dx.doi.org/10.3389/fmicb.2022.831659 Text en Copyright © 2022 Xia, Cheng and Zhong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xia, Yucheng
Cheng, Huanyu
Zhong, Jiang
Hybrid Sequencing Resolved Inverted Terminal Repeats in the Genome of Megavirus Baoshan
title Hybrid Sequencing Resolved Inverted Terminal Repeats in the Genome of Megavirus Baoshan
title_full Hybrid Sequencing Resolved Inverted Terminal Repeats in the Genome of Megavirus Baoshan
title_fullStr Hybrid Sequencing Resolved Inverted Terminal Repeats in the Genome of Megavirus Baoshan
title_full_unstemmed Hybrid Sequencing Resolved Inverted Terminal Repeats in the Genome of Megavirus Baoshan
title_short Hybrid Sequencing Resolved Inverted Terminal Repeats in the Genome of Megavirus Baoshan
title_sort hybrid sequencing resolved inverted terminal repeats in the genome of megavirus baoshan
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127612/
https://www.ncbi.nlm.nih.gov/pubmed/35620092
http://dx.doi.org/10.3389/fmicb.2022.831659
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