Isolation and RNA sequencing of single nuclei from Drosophila tissues

Many insect cells are encapsulated within the exoskeleton and cannot be dissociated intact, making them inaccessible to single-cell transcriptomic profiling. We have used single-nucleus RNA sequencing to extract transcriptomic information from multiple Drosophila tissues. Here, we describe procedure...

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Detalles Bibliográficos
Autores principales: McLaughlin, Colleen N., Qi, Yanyan, Quake, Stephen R., Luo, Liqun, Li, Hongjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127693/
https://www.ncbi.nlm.nih.gov/pubmed/35620068
http://dx.doi.org/10.1016/j.xpro.2022.101417

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