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The First Cbk-Like Phage Infecting Erythrobacter, Representing a Novel Siphoviral Genus

Erythrobacter is an important and widespread bacterial genus in the ocean. However, our knowledge about their phages is still rare. Here, a novel lytic phage vB_EliS-L02, infecting Erythrobacter litoralis DSM 8509, was isolated and purified from Sanggou Bay seawater, China. Morphological observation...

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Autores principales: Li, Xuejing, Guo, Ruizhe, Zou, Xiao, Yao, Yanyan, Lu, Longfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127768/
https://www.ncbi.nlm.nih.gov/pubmed/35620087
http://dx.doi.org/10.3389/fmicb.2022.861793
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author Li, Xuejing
Guo, Ruizhe
Zou, Xiao
Yao, Yanyan
Lu, Longfei
author_facet Li, Xuejing
Guo, Ruizhe
Zou, Xiao
Yao, Yanyan
Lu, Longfei
author_sort Li, Xuejing
collection PubMed
description Erythrobacter is an important and widespread bacterial genus in the ocean. However, our knowledge about their phages is still rare. Here, a novel lytic phage vB_EliS-L02, infecting Erythrobacter litoralis DSM 8509, was isolated and purified from Sanggou Bay seawater, China. Morphological observation revealed that the phage belonged to Cbk-like siphovirus, with a long prolate head and a long tail. The host range test showed that phage vB_EliS-L02 could only infect a few strains of Erythrobacter, demonstrating its potential narrow-host range. The genome size of vB_EliS-L02 was 150,063 bp with a G+C content of 59.43%, encoding 231 putative open reading frames (ORFs), but only 47 were predicted to be functional domains. Fourteen auxiliary metabolic genes were identified, including phoH that may confer vB_EliS-L02 the advantage of regulating phosphate uptake and metabolism under a phosphate-limiting condition. Genomic and phylogenetic analyses indicated that vB_EliS-L02 was most closely related to the genus Lacusarxvirus with low similarity (shared genes < 30%, and average nucleotide sequence identity < 70%), distantly from other reported phages, and could be grouped into a novel viral genus cluster, in this study as Eliscbkvirus. Meanwhile, the genus Eliscbkvirus and Lacusarxvirus stand out from other siphoviral genera and could represent a novel subfamily within Siphoviridae, named Dolichocephalovirinae-II. Being a representative of an understudied viral group with manifold adaptations to the host, phage vB_EliS-L02 could improve our understanding of the virus–host interactions and provide reference information for viral metagenomic analysis in the ocean.
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spelling pubmed-91277682022-05-25 The First Cbk-Like Phage Infecting Erythrobacter, Representing a Novel Siphoviral Genus Li, Xuejing Guo, Ruizhe Zou, Xiao Yao, Yanyan Lu, Longfei Front Microbiol Microbiology Erythrobacter is an important and widespread bacterial genus in the ocean. However, our knowledge about their phages is still rare. Here, a novel lytic phage vB_EliS-L02, infecting Erythrobacter litoralis DSM 8509, was isolated and purified from Sanggou Bay seawater, China. Morphological observation revealed that the phage belonged to Cbk-like siphovirus, with a long prolate head and a long tail. The host range test showed that phage vB_EliS-L02 could only infect a few strains of Erythrobacter, demonstrating its potential narrow-host range. The genome size of vB_EliS-L02 was 150,063 bp with a G+C content of 59.43%, encoding 231 putative open reading frames (ORFs), but only 47 were predicted to be functional domains. Fourteen auxiliary metabolic genes were identified, including phoH that may confer vB_EliS-L02 the advantage of regulating phosphate uptake and metabolism under a phosphate-limiting condition. Genomic and phylogenetic analyses indicated that vB_EliS-L02 was most closely related to the genus Lacusarxvirus with low similarity (shared genes < 30%, and average nucleotide sequence identity < 70%), distantly from other reported phages, and could be grouped into a novel viral genus cluster, in this study as Eliscbkvirus. Meanwhile, the genus Eliscbkvirus and Lacusarxvirus stand out from other siphoviral genera and could represent a novel subfamily within Siphoviridae, named Dolichocephalovirinae-II. Being a representative of an understudied viral group with manifold adaptations to the host, phage vB_EliS-L02 could improve our understanding of the virus–host interactions and provide reference information for viral metagenomic analysis in the ocean. Frontiers Media S.A. 2022-05-10 /pmc/articles/PMC9127768/ /pubmed/35620087 http://dx.doi.org/10.3389/fmicb.2022.861793 Text en Copyright © 2022 Li, Guo, Zou, Yao and Lu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Xuejing
Guo, Ruizhe
Zou, Xiao
Yao, Yanyan
Lu, Longfei
The First Cbk-Like Phage Infecting Erythrobacter, Representing a Novel Siphoviral Genus
title The First Cbk-Like Phage Infecting Erythrobacter, Representing a Novel Siphoviral Genus
title_full The First Cbk-Like Phage Infecting Erythrobacter, Representing a Novel Siphoviral Genus
title_fullStr The First Cbk-Like Phage Infecting Erythrobacter, Representing a Novel Siphoviral Genus
title_full_unstemmed The First Cbk-Like Phage Infecting Erythrobacter, Representing a Novel Siphoviral Genus
title_short The First Cbk-Like Phage Infecting Erythrobacter, Representing a Novel Siphoviral Genus
title_sort first cbk-like phage infecting erythrobacter, representing a novel siphoviral genus
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127768/
https://www.ncbi.nlm.nih.gov/pubmed/35620087
http://dx.doi.org/10.3389/fmicb.2022.861793
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