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Tetrazine-Ligated CRISPR sgRNAs for Efficient Genome Editing

[Image: see text] CRISPR-Cas technology has revolutionized genome editing. Its broad and fast-growing application in biomedical research and therapeutics has led to increased demand for guide RNAs. The synthesis of chemically modified single-guide RNAs (sgRNAs) containing >100 nucleotides remains...

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Detalles Bibliográficos
Autores principales:	Chen, Zexiang, Devi, Gitali, Arif, Amena, Zamore, Phillip D., Sontheimer, Erik J., Watts, Jonathan K.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2022
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127786/ https://www.ncbi.nlm.nih.gov/pubmed/35446558 http://dx.doi.org/10.1021/acschembio.2c00116

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