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Performance of SARS-CoV-2 Antigen-Detecting Rapid Diagnostic Tests for Omicron and Other Variants of Concern

The SARS-CoV-2 antigen-detecting rapid diagnostic test (Ag-RDTs) is an easy-to-use diagnostic tool to identify the contagious individuals and reduce the new infections. However, to be effective, Ag-RDTs require the detection of distinct variants of concern (VOC) with high analytical sensitivity. Her...

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Autores principales: Raïch-Regué, Dàlia, Muñoz-Basagoiti, Jordana, Perez-Zsolt, Daniel, Noguera-Julian, Marc, Pradenas, Edwards, Riveira-Muñoz, Eva, Giménez, Neus, Carabaza, Assumpta, Giménez, Francesc, Saludes, Verónica, Martró, Elisa, Robert, Neus, Blanco, Ignacio, Paredes, Roger, Ruiz, Lidia, Ballana, Ester, Clotet, Bonaventura, Blanco, Julià, Izquierdo-Useros, Nuria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127986/
https://www.ncbi.nlm.nih.gov/pubmed/35620108
http://dx.doi.org/10.3389/fmicb.2022.810576
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author Raïch-Regué, Dàlia
Muñoz-Basagoiti, Jordana
Perez-Zsolt, Daniel
Noguera-Julian, Marc
Pradenas, Edwards
Riveira-Muñoz, Eva
Giménez, Neus
Carabaza, Assumpta
Giménez, Francesc
Saludes, Verónica
Martró, Elisa
Robert, Neus
Blanco, Ignacio
Paredes, Roger
Ruiz, Lidia
Ballana, Ester
Clotet, Bonaventura
Blanco, Julià
Izquierdo-Useros, Nuria
author_facet Raïch-Regué, Dàlia
Muñoz-Basagoiti, Jordana
Perez-Zsolt, Daniel
Noguera-Julian, Marc
Pradenas, Edwards
Riveira-Muñoz, Eva
Giménez, Neus
Carabaza, Assumpta
Giménez, Francesc
Saludes, Verónica
Martró, Elisa
Robert, Neus
Blanco, Ignacio
Paredes, Roger
Ruiz, Lidia
Ballana, Ester
Clotet, Bonaventura
Blanco, Julià
Izquierdo-Useros, Nuria
author_sort Raïch-Regué, Dàlia
collection PubMed
description The SARS-CoV-2 antigen-detecting rapid diagnostic test (Ag-RDTs) is an easy-to-use diagnostic tool to identify the contagious individuals and reduce the new infections. However, to be effective, Ag-RDTs require the detection of distinct variants of concern (VOC) with high analytical sensitivity. Here, we found that the VOC diverge at the nucleocapsid protein used by four commercial Ag-RDTs for the viral detection. Relative to the original D614G variant, there was a 10-fold loss of detection for the Delta and Alpha variants in certain Ag-RDTs, a reduction above the threshold required to isolate the viable virus. However, Beta and Omicron variants did not lose the detection capacity. As the new VOC arise, successful contact tracing requires continuous monitoring of Ag-RDTs performance.
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spelling pubmed-91279862022-05-25 Performance of SARS-CoV-2 Antigen-Detecting Rapid Diagnostic Tests for Omicron and Other Variants of Concern Raïch-Regué, Dàlia Muñoz-Basagoiti, Jordana Perez-Zsolt, Daniel Noguera-Julian, Marc Pradenas, Edwards Riveira-Muñoz, Eva Giménez, Neus Carabaza, Assumpta Giménez, Francesc Saludes, Verónica Martró, Elisa Robert, Neus Blanco, Ignacio Paredes, Roger Ruiz, Lidia Ballana, Ester Clotet, Bonaventura Blanco, Julià Izquierdo-Useros, Nuria Front Microbiol Microbiology The SARS-CoV-2 antigen-detecting rapid diagnostic test (Ag-RDTs) is an easy-to-use diagnostic tool to identify the contagious individuals and reduce the new infections. However, to be effective, Ag-RDTs require the detection of distinct variants of concern (VOC) with high analytical sensitivity. Here, we found that the VOC diverge at the nucleocapsid protein used by four commercial Ag-RDTs for the viral detection. Relative to the original D614G variant, there was a 10-fold loss of detection for the Delta and Alpha variants in certain Ag-RDTs, a reduction above the threshold required to isolate the viable virus. However, Beta and Omicron variants did not lose the detection capacity. As the new VOC arise, successful contact tracing requires continuous monitoring of Ag-RDTs performance. Frontiers Media S.A. 2022-05-10 /pmc/articles/PMC9127986/ /pubmed/35620108 http://dx.doi.org/10.3389/fmicb.2022.810576 Text en Copyright © 2022 Raïch-Regué, Muñoz-Basagoiti, Perez-Zsolt, Noguera-Julian, Pradenas, Riveira-Muñoz, Giménez, Carabaza, Giménez, Saludes, Martró, Robert, Blanco, Paredes, Ruiz, Ballana, Clotet, Blanco and Izquierdo-Useros. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Raïch-Regué, Dàlia
Muñoz-Basagoiti, Jordana
Perez-Zsolt, Daniel
Noguera-Julian, Marc
Pradenas, Edwards
Riveira-Muñoz, Eva
Giménez, Neus
Carabaza, Assumpta
Giménez, Francesc
Saludes, Verónica
Martró, Elisa
Robert, Neus
Blanco, Ignacio
Paredes, Roger
Ruiz, Lidia
Ballana, Ester
Clotet, Bonaventura
Blanco, Julià
Izquierdo-Useros, Nuria
Performance of SARS-CoV-2 Antigen-Detecting Rapid Diagnostic Tests for Omicron and Other Variants of Concern
title Performance of SARS-CoV-2 Antigen-Detecting Rapid Diagnostic Tests for Omicron and Other Variants of Concern
title_full Performance of SARS-CoV-2 Antigen-Detecting Rapid Diagnostic Tests for Omicron and Other Variants of Concern
title_fullStr Performance of SARS-CoV-2 Antigen-Detecting Rapid Diagnostic Tests for Omicron and Other Variants of Concern
title_full_unstemmed Performance of SARS-CoV-2 Antigen-Detecting Rapid Diagnostic Tests for Omicron and Other Variants of Concern
title_short Performance of SARS-CoV-2 Antigen-Detecting Rapid Diagnostic Tests for Omicron and Other Variants of Concern
title_sort performance of sars-cov-2 antigen-detecting rapid diagnostic tests for omicron and other variants of concern
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127986/
https://www.ncbi.nlm.nih.gov/pubmed/35620108
http://dx.doi.org/10.3389/fmicb.2022.810576
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