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Curcumin and Thymoquinone Combination Attenuates Breast Cancer Cell Lines’ Progression
Breast cancer is the most harmful malignancy in women worldwide. Therefore, in the current study, we investigated the combinatory effect of natural bioactive compounds, including curcumin (Cur) and thymoquinone (TQ), on MCF7 and MDA-MB-231 breast cancer cell lines’ progression. We investigated the F...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9128062/ https://www.ncbi.nlm.nih.gov/pubmed/35583244 http://dx.doi.org/10.1177/15347354221099537 |
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author | El-Far, Ali H. Saddiq, Amna A. Mohamed, Shymaa A. Almaghrabi, Omar A. Mousa, Shaker A. |
author_facet | El-Far, Ali H. Saddiq, Amna A. Mohamed, Shymaa A. Almaghrabi, Omar A. Mousa, Shaker A. |
author_sort | El-Far, Ali H. |
collection | PubMed |
description | Breast cancer is the most harmful malignancy in women worldwide. Therefore, in the current study, we investigated the combinatory effect of natural bioactive compounds, including curcumin (Cur) and thymoquinone (TQ), on MCF7 and MDA-MB-231 breast cancer cell lines’ progression. We investigated the Fa values and combination index of Cur and TQ in this context. Moreover, cytotoxicity percentages, annexin-V, proliferation, colony formation, and migration assays were used along with cell cycle analysis. In addition, caspase-3, phosphatidylinositol 3-kinase (PI3K), and protein kinase B (AKT) protein levels were determined by ELISA assessment. The results showed that Cur, TQ, and Cur + TQ induced apoptosis with cell cycle arrest and decreased cell proliferation, colony formation, and migration activities. Cur + TQ combination significantly increased caspase-3 and decreased PI3K and AKT protein levels. These results suggest the promising anticancer benefit of the Cur and TQ combination against breast cancer. |
format | Online Article Text |
id | pubmed-9128062 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-91280622022-05-25 Curcumin and Thymoquinone Combination Attenuates Breast Cancer Cell Lines’ Progression El-Far, Ali H. Saddiq, Amna A. Mohamed, Shymaa A. Almaghrabi, Omar A. Mousa, Shaker A. Integr Cancer Ther Research Article Breast cancer is the most harmful malignancy in women worldwide. Therefore, in the current study, we investigated the combinatory effect of natural bioactive compounds, including curcumin (Cur) and thymoquinone (TQ), on MCF7 and MDA-MB-231 breast cancer cell lines’ progression. We investigated the Fa values and combination index of Cur and TQ in this context. Moreover, cytotoxicity percentages, annexin-V, proliferation, colony formation, and migration assays were used along with cell cycle analysis. In addition, caspase-3, phosphatidylinositol 3-kinase (PI3K), and protein kinase B (AKT) protein levels were determined by ELISA assessment. The results showed that Cur, TQ, and Cur + TQ induced apoptosis with cell cycle arrest and decreased cell proliferation, colony formation, and migration activities. Cur + TQ combination significantly increased caspase-3 and decreased PI3K and AKT protein levels. These results suggest the promising anticancer benefit of the Cur and TQ combination against breast cancer. SAGE Publications 2022-05-18 /pmc/articles/PMC9128062/ /pubmed/35583244 http://dx.doi.org/10.1177/15347354221099537 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Research Article El-Far, Ali H. Saddiq, Amna A. Mohamed, Shymaa A. Almaghrabi, Omar A. Mousa, Shaker A. Curcumin and Thymoquinone Combination Attenuates Breast Cancer Cell Lines’ Progression |
title | Curcumin and Thymoquinone Combination Attenuates Breast Cancer Cell Lines’ Progression |
title_full | Curcumin and Thymoquinone Combination Attenuates Breast Cancer Cell Lines’ Progression |
title_fullStr | Curcumin and Thymoquinone Combination Attenuates Breast Cancer Cell Lines’ Progression |
title_full_unstemmed | Curcumin and Thymoquinone Combination Attenuates Breast Cancer Cell Lines’ Progression |
title_short | Curcumin and Thymoquinone Combination Attenuates Breast Cancer Cell Lines’ Progression |
title_sort | curcumin and thymoquinone combination attenuates breast cancer cell lines’ progression |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9128062/ https://www.ncbi.nlm.nih.gov/pubmed/35583244 http://dx.doi.org/10.1177/15347354221099537 |
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