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Anticancer effect of locally applicable aptamer‐conjugated gemcitabine‐loaded atelocollagen patch in pancreatic cancer patient–derived xenograft models

We investigated the anticancer effect of the aptamer‐conjugated gemcitabine‐loaded atelocollagen patch in a pancreatic cancer patient–derived xenograft (PDX) model to propose a future potential adjuvant surgical strategy during curative pancreatic resection for pancreatic cancer. A pancreatic cancer...

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Autores principales: Hong, Seung Soo, Lee, Sena, Lee, Sung Hwan, Kim, Seonhowa, Kim, Doyoung, Park, Hanseul, Lee, Jongook, Lee, Jung Hwan, Kang, Chang Moo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9128157/
https://www.ncbi.nlm.nih.gov/pubmed/35243724
http://dx.doi.org/10.1111/cas.15318
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author Hong, Seung Soo
Lee, Sena
Lee, Sung Hwan
Kim, Seonhowa
Kim, Doyoung
Park, Hanseul
Lee, Jongook
Lee, Jung Hwan
Kang, Chang Moo
author_facet Hong, Seung Soo
Lee, Sena
Lee, Sung Hwan
Kim, Seonhowa
Kim, Doyoung
Park, Hanseul
Lee, Jongook
Lee, Jung Hwan
Kang, Chang Moo
author_sort Hong, Seung Soo
collection PubMed
description We investigated the anticancer effect of the aptamer‐conjugated gemcitabine‐loaded atelocollagen patch in a pancreatic cancer patient–derived xenograft (PDX) model to propose a future potential adjuvant surgical strategy during curative pancreatic resection for pancreatic cancer. A pancreatic cancer PDX model was established. Animals were grouped randomly into a no‐treatment control group; treatment group treated with intraperitoneal gemcitabine injection (IP‐GEM) or aptamer‐conjugated gemcitabine (APT:GEM); and transplant with three kinds of patches: atelocollagen‐aptamer‐gemcitabine (patch I), atelocollagen‐inactive aptamer‐gemcitabine (patch II), and atelocollagen‐gemcitabine (patch III). Tumor volumes and response were evaluated based on histological analysis by H&E staining and Immunohistochemistry (IHC) was performed. Anticancer therapy–related toxicity was evaluated by hematologic findings. The patch I group showed the most significant reduction of tumor growth rate, compared with the no‐treatment group (p < 0.05). However, other treatment groups were not found to show significant reduction in tumor growth rate (0.05 < p < 0.1). There was no microscopic evidence suggesting potential toxicity, such as inflammation, nor necrotic changes in liver, lung, kidney, and spleen tissue. In addition, no leukopenia, anemia, or neutropenia was observed in the patch I group. This implantable aptamer‐drug conjugate system is thought to be a new surgical strategy to augment the oncologic significance of margin‐negative resection in treating pancreatic cancer in near future.
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spelling pubmed-91281572022-05-25 Anticancer effect of locally applicable aptamer‐conjugated gemcitabine‐loaded atelocollagen patch in pancreatic cancer patient–derived xenograft models Hong, Seung Soo Lee, Sena Lee, Sung Hwan Kim, Seonhowa Kim, Doyoung Park, Hanseul Lee, Jongook Lee, Jung Hwan Kang, Chang Moo Cancer Sci ORIGINAL ARTICLES We investigated the anticancer effect of the aptamer‐conjugated gemcitabine‐loaded atelocollagen patch in a pancreatic cancer patient–derived xenograft (PDX) model to propose a future potential adjuvant surgical strategy during curative pancreatic resection for pancreatic cancer. A pancreatic cancer PDX model was established. Animals were grouped randomly into a no‐treatment control group; treatment group treated with intraperitoneal gemcitabine injection (IP‐GEM) or aptamer‐conjugated gemcitabine (APT:GEM); and transplant with three kinds of patches: atelocollagen‐aptamer‐gemcitabine (patch I), atelocollagen‐inactive aptamer‐gemcitabine (patch II), and atelocollagen‐gemcitabine (patch III). Tumor volumes and response were evaluated based on histological analysis by H&E staining and Immunohistochemistry (IHC) was performed. Anticancer therapy–related toxicity was evaluated by hematologic findings. The patch I group showed the most significant reduction of tumor growth rate, compared with the no‐treatment group (p < 0.05). However, other treatment groups were not found to show significant reduction in tumor growth rate (0.05 < p < 0.1). There was no microscopic evidence suggesting potential toxicity, such as inflammation, nor necrotic changes in liver, lung, kidney, and spleen tissue. In addition, no leukopenia, anemia, or neutropenia was observed in the patch I group. This implantable aptamer‐drug conjugate system is thought to be a new surgical strategy to augment the oncologic significance of margin‐negative resection in treating pancreatic cancer in near future. John Wiley and Sons Inc. 2022-03-24 2022-05 /pmc/articles/PMC9128157/ /pubmed/35243724 http://dx.doi.org/10.1111/cas.15318 Text en © 2022 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle ORIGINAL ARTICLES
Hong, Seung Soo
Lee, Sena
Lee, Sung Hwan
Kim, Seonhowa
Kim, Doyoung
Park, Hanseul
Lee, Jongook
Lee, Jung Hwan
Kang, Chang Moo
Anticancer effect of locally applicable aptamer‐conjugated gemcitabine‐loaded atelocollagen patch in pancreatic cancer patient–derived xenograft models
title Anticancer effect of locally applicable aptamer‐conjugated gemcitabine‐loaded atelocollagen patch in pancreatic cancer patient–derived xenograft models
title_full Anticancer effect of locally applicable aptamer‐conjugated gemcitabine‐loaded atelocollagen patch in pancreatic cancer patient–derived xenograft models
title_fullStr Anticancer effect of locally applicable aptamer‐conjugated gemcitabine‐loaded atelocollagen patch in pancreatic cancer patient–derived xenograft models
title_full_unstemmed Anticancer effect of locally applicable aptamer‐conjugated gemcitabine‐loaded atelocollagen patch in pancreatic cancer patient–derived xenograft models
title_short Anticancer effect of locally applicable aptamer‐conjugated gemcitabine‐loaded atelocollagen patch in pancreatic cancer patient–derived xenograft models
title_sort anticancer effect of locally applicable aptamer‐conjugated gemcitabine‐loaded atelocollagen patch in pancreatic cancer patient–derived xenograft models
topic ORIGINAL ARTICLES
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9128157/
https://www.ncbi.nlm.nih.gov/pubmed/35243724
http://dx.doi.org/10.1111/cas.15318
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