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Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach

The suprachiasmatic nuclei (SCN) of the hypothalamus harbor the central clock of the circadian system, which gradually matures during the perinatal period. In this study, time-resolved transcriptomic and proteomic approaches were used to describe fetal SCN tissue-level rhythms before rhythms in cloc...

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Autores principales: Greiner, Philipp, Houdek, Pavel, Sládek, Martin, Sumová, Alena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9129005/
https://www.ncbi.nlm.nih.gov/pubmed/35609026
http://dx.doi.org/10.1371/journal.pbio.3001637
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author Greiner, Philipp
Houdek, Pavel
Sládek, Martin
Sumová, Alena
author_facet Greiner, Philipp
Houdek, Pavel
Sládek, Martin
Sumová, Alena
author_sort Greiner, Philipp
collection PubMed
description The suprachiasmatic nuclei (SCN) of the hypothalamus harbor the central clock of the circadian system, which gradually matures during the perinatal period. In this study, time-resolved transcriptomic and proteomic approaches were used to describe fetal SCN tissue-level rhythms before rhythms in clock gene expression develop. Pregnant rats were maintained in constant darkness and had intact SCN, or their SCN were lesioned and behavioral rhythm was imposed by temporal restriction of food availability. Model-selecting tools dryR and CompareRhythms identified sets of genes in the fetal SCN that were rhythmic in the absence of the fetal canonical clock. Subsets of rhythmically expressed genes were assigned to groups of fetuses from mothers with either intact or lesioned SCN, or both groups. Enrichment analysis for GO terms and signaling pathways revealed that neurodevelopment and cell-to-cell signaling were significantly enriched within the subsets of genes that were rhythmic in response to distinct maternal signals. The findings discovered a previously unexpected breadth of rhythmicity in the fetal SCN at a developmental stage when the canonical clock has not yet developed at the tissue level and thus likely represents responses to rhythmic maternal signals.
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spelling pubmed-91290052022-05-25 Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach Greiner, Philipp Houdek, Pavel Sládek, Martin Sumová, Alena PLoS Biol Research Article The suprachiasmatic nuclei (SCN) of the hypothalamus harbor the central clock of the circadian system, which gradually matures during the perinatal period. In this study, time-resolved transcriptomic and proteomic approaches were used to describe fetal SCN tissue-level rhythms before rhythms in clock gene expression develop. Pregnant rats were maintained in constant darkness and had intact SCN, or their SCN were lesioned and behavioral rhythm was imposed by temporal restriction of food availability. Model-selecting tools dryR and CompareRhythms identified sets of genes in the fetal SCN that were rhythmic in the absence of the fetal canonical clock. Subsets of rhythmically expressed genes were assigned to groups of fetuses from mothers with either intact or lesioned SCN, or both groups. Enrichment analysis for GO terms and signaling pathways revealed that neurodevelopment and cell-to-cell signaling were significantly enriched within the subsets of genes that were rhythmic in response to distinct maternal signals. The findings discovered a previously unexpected breadth of rhythmicity in the fetal SCN at a developmental stage when the canonical clock has not yet developed at the tissue level and thus likely represents responses to rhythmic maternal signals. Public Library of Science 2022-05-24 /pmc/articles/PMC9129005/ /pubmed/35609026 http://dx.doi.org/10.1371/journal.pbio.3001637 Text en © 2022 Greiner et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Greiner, Philipp
Houdek, Pavel
Sládek, Martin
Sumová, Alena
Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach
title Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach
title_full Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach
title_fullStr Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach
title_full_unstemmed Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach
title_short Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach
title_sort early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9129005/
https://www.ncbi.nlm.nih.gov/pubmed/35609026
http://dx.doi.org/10.1371/journal.pbio.3001637
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