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Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach
The suprachiasmatic nuclei (SCN) of the hypothalamus harbor the central clock of the circadian system, which gradually matures during the perinatal period. In this study, time-resolved transcriptomic and proteomic approaches were used to describe fetal SCN tissue-level rhythms before rhythms in cloc...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9129005/ https://www.ncbi.nlm.nih.gov/pubmed/35609026 http://dx.doi.org/10.1371/journal.pbio.3001637 |
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author | Greiner, Philipp Houdek, Pavel Sládek, Martin Sumová, Alena |
author_facet | Greiner, Philipp Houdek, Pavel Sládek, Martin Sumová, Alena |
author_sort | Greiner, Philipp |
collection | PubMed |
description | The suprachiasmatic nuclei (SCN) of the hypothalamus harbor the central clock of the circadian system, which gradually matures during the perinatal period. In this study, time-resolved transcriptomic and proteomic approaches were used to describe fetal SCN tissue-level rhythms before rhythms in clock gene expression develop. Pregnant rats were maintained in constant darkness and had intact SCN, or their SCN were lesioned and behavioral rhythm was imposed by temporal restriction of food availability. Model-selecting tools dryR and CompareRhythms identified sets of genes in the fetal SCN that were rhythmic in the absence of the fetal canonical clock. Subsets of rhythmically expressed genes were assigned to groups of fetuses from mothers with either intact or lesioned SCN, or both groups. Enrichment analysis for GO terms and signaling pathways revealed that neurodevelopment and cell-to-cell signaling were significantly enriched within the subsets of genes that were rhythmic in response to distinct maternal signals. The findings discovered a previously unexpected breadth of rhythmicity in the fetal SCN at a developmental stage when the canonical clock has not yet developed at the tissue level and thus likely represents responses to rhythmic maternal signals. |
format | Online Article Text |
id | pubmed-9129005 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-91290052022-05-25 Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach Greiner, Philipp Houdek, Pavel Sládek, Martin Sumová, Alena PLoS Biol Research Article The suprachiasmatic nuclei (SCN) of the hypothalamus harbor the central clock of the circadian system, which gradually matures during the perinatal period. In this study, time-resolved transcriptomic and proteomic approaches were used to describe fetal SCN tissue-level rhythms before rhythms in clock gene expression develop. Pregnant rats were maintained in constant darkness and had intact SCN, or their SCN were lesioned and behavioral rhythm was imposed by temporal restriction of food availability. Model-selecting tools dryR and CompareRhythms identified sets of genes in the fetal SCN that were rhythmic in the absence of the fetal canonical clock. Subsets of rhythmically expressed genes were assigned to groups of fetuses from mothers with either intact or lesioned SCN, or both groups. Enrichment analysis for GO terms and signaling pathways revealed that neurodevelopment and cell-to-cell signaling were significantly enriched within the subsets of genes that were rhythmic in response to distinct maternal signals. The findings discovered a previously unexpected breadth of rhythmicity in the fetal SCN at a developmental stage when the canonical clock has not yet developed at the tissue level and thus likely represents responses to rhythmic maternal signals. Public Library of Science 2022-05-24 /pmc/articles/PMC9129005/ /pubmed/35609026 http://dx.doi.org/10.1371/journal.pbio.3001637 Text en © 2022 Greiner et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Greiner, Philipp Houdek, Pavel Sládek, Martin Sumová, Alena Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach |
title | Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach |
title_full | Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach |
title_fullStr | Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach |
title_full_unstemmed | Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach |
title_short | Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach |
title_sort | early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9129005/ https://www.ncbi.nlm.nih.gov/pubmed/35609026 http://dx.doi.org/10.1371/journal.pbio.3001637 |
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