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Expression and functional maintenance of volume-regulated anion channels in myometrial smooth muscles of pregnant mice

Pregnancy causes changes in the uterus, such as increased cell volume and altered water content. However, the mechanisms that protect the structure and maintain the function of uterine smooth muscle cells against these changes during pregnancy have not been clarified. This study focused on the volum...

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Autores principales: Yamada, Kazutaka, Ding, Wei-Guang, Omatsu-Kanbe, Mariko, Toyoda, Futoshi, Tsuji, Shunichiro, Katsura, Daisuke, Kimura, Fuminori, Matsuura, Hiroshi, Murakami, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130036/
https://www.ncbi.nlm.nih.gov/pubmed/34789619
http://dx.doi.org/10.1538/expanim.21-0111
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author Yamada, Kazutaka
Ding, Wei-Guang
Omatsu-Kanbe, Mariko
Toyoda, Futoshi
Tsuji, Shunichiro
Katsura, Daisuke
Kimura, Fuminori
Matsuura, Hiroshi
Murakami, Takashi
author_facet Yamada, Kazutaka
Ding, Wei-Guang
Omatsu-Kanbe, Mariko
Toyoda, Futoshi
Tsuji, Shunichiro
Katsura, Daisuke
Kimura, Fuminori
Matsuura, Hiroshi
Murakami, Takashi
author_sort Yamada, Kazutaka
collection PubMed
description Pregnancy causes changes in the uterus, such as increased cell volume and altered water content. However, the mechanisms that protect the structure and maintain the function of uterine smooth muscle cells against these changes during pregnancy have not been clarified. This study focused on the volume-regulated anion channel (VRAC), which opens with cell swelling under low osmotic pressure and releases Cl(–) ions and various organic osmolytes to resist cell swelling and regulates a wide range of biological processes such as cell death. In this study, myometrial smooth muscle (MSM) tissues and cells (MSMCs) were collected from non-pregnant and pregnant mice. Using western blotting and immunocytochemistry, leucine-rich repeat containing protein 8A (LRRC8A), an essential membrane protein that constitutes part of the VRAC, was determined to be diffused throughout MSMCs including in the cell membrane. Patch-clamp experiments were performed to investigate the electrophysiology of swelling-induced Cl(–) currents (I(Cl, swell)) mediated by the VRAC. No significant changes between non-pregnancy and pregnancy groups were observed in either the expression density of LRRC8A or the current density of I(Cl, swell), however the presence of LRRC8A on the cell membrane was significantly increased in the third trimester of pregnancy compared to the non-pregnancy. This study suggests that the VRAC may play a role, such as maintaining cellular homeostasis in the pregnant MSM.
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spelling pubmed-91300362022-06-09 Expression and functional maintenance of volume-regulated anion channels in myometrial smooth muscles of pregnant mice Yamada, Kazutaka Ding, Wei-Guang Omatsu-Kanbe, Mariko Toyoda, Futoshi Tsuji, Shunichiro Katsura, Daisuke Kimura, Fuminori Matsuura, Hiroshi Murakami, Takashi Exp Anim Original Pregnancy causes changes in the uterus, such as increased cell volume and altered water content. However, the mechanisms that protect the structure and maintain the function of uterine smooth muscle cells against these changes during pregnancy have not been clarified. This study focused on the volume-regulated anion channel (VRAC), which opens with cell swelling under low osmotic pressure and releases Cl(–) ions and various organic osmolytes to resist cell swelling and regulates a wide range of biological processes such as cell death. In this study, myometrial smooth muscle (MSM) tissues and cells (MSMCs) were collected from non-pregnant and pregnant mice. Using western blotting and immunocytochemistry, leucine-rich repeat containing protein 8A (LRRC8A), an essential membrane protein that constitutes part of the VRAC, was determined to be diffused throughout MSMCs including in the cell membrane. Patch-clamp experiments were performed to investigate the electrophysiology of swelling-induced Cl(–) currents (I(Cl, swell)) mediated by the VRAC. No significant changes between non-pregnancy and pregnancy groups were observed in either the expression density of LRRC8A or the current density of I(Cl, swell), however the presence of LRRC8A on the cell membrane was significantly increased in the third trimester of pregnancy compared to the non-pregnancy. This study suggests that the VRAC may play a role, such as maintaining cellular homeostasis in the pregnant MSM. Japanese Association for Laboratory Animal Science 2021-11-16 2022 /pmc/articles/PMC9130036/ /pubmed/34789619 http://dx.doi.org/10.1538/expanim.21-0111 Text en ©2022 Japanese Association for Laboratory Animal Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original
Yamada, Kazutaka
Ding, Wei-Guang
Omatsu-Kanbe, Mariko
Toyoda, Futoshi
Tsuji, Shunichiro
Katsura, Daisuke
Kimura, Fuminori
Matsuura, Hiroshi
Murakami, Takashi
Expression and functional maintenance of volume-regulated anion channels in myometrial smooth muscles of pregnant mice
title Expression and functional maintenance of volume-regulated anion channels in myometrial smooth muscles of pregnant mice
title_full Expression and functional maintenance of volume-regulated anion channels in myometrial smooth muscles of pregnant mice
title_fullStr Expression and functional maintenance of volume-regulated anion channels in myometrial smooth muscles of pregnant mice
title_full_unstemmed Expression and functional maintenance of volume-regulated anion channels in myometrial smooth muscles of pregnant mice
title_short Expression and functional maintenance of volume-regulated anion channels in myometrial smooth muscles of pregnant mice
title_sort expression and functional maintenance of volume-regulated anion channels in myometrial smooth muscles of pregnant mice
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130036/
https://www.ncbi.nlm.nih.gov/pubmed/34789619
http://dx.doi.org/10.1538/expanim.21-0111
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