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Simplifying quantitative measurement of free radical species using an X-band EPR spectrometer

The quantitative measurement of free radicals in liquid using an X-band electron paramagnetic resonance (EPR) was systematized. Quantification of free radicals by EPR requires a standard sample that contains a known spin amount/concentration. When satisfactory reproducibility of the sample material,...

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Autores principales: Makino, Yusuke, Ueno, Megumi, Shoji, Yoshimi, Nyui, Minako, Nakanishi, Ikuo, Fukui, Koji, Matsumoto, Ken-ichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Society for Free Radical Research Japan 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130060/
https://www.ncbi.nlm.nih.gov/pubmed/35692674
http://dx.doi.org/10.3164/jcbn.21-83
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author Makino, Yusuke
Ueno, Megumi
Shoji, Yoshimi
Nyui, Minako
Nakanishi, Ikuo
Fukui, Koji
Matsumoto, Ken-ichiro
author_facet Makino, Yusuke
Ueno, Megumi
Shoji, Yoshimi
Nyui, Minako
Nakanishi, Ikuo
Fukui, Koji
Matsumoto, Ken-ichiro
author_sort Makino, Yusuke
collection PubMed
description The quantitative measurement of free radicals in liquid using an X-band electron paramagnetic resonance (EPR) was systematized. Quantification of free radicals by EPR requires a standard sample that contains a known spin amount/concentration. When satisfactory reproducibility of the sample material, volume, shape, and positioning in the cavity for EPR measurements can be guaranteed, a sample tested and a standard can be directly compared and the process of quantification can be simplified. The purpose of this study was to simplify manual quantitative EPR measurement. A suitable sample volume for achieving a stable EPR intensity was estimated. The effects of different solvents on the EPR sensitivity were compared. The stability and reproducibility of the EPR intensity of standard nitroxyl radical solutions were compared among different types of sample tubes. When the sample tubes, sample volumes, and/or solvents were the same, the EPR intensity was reproduced with an error of 2% or less for μM samples. The quantified sample and the standard sample in the same solvent and the same volume drawn into the same sample tube was able to be directly compared. The standard sample for quantification should be measured just before or after every daily experiment.
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spelling pubmed-91300602022-06-09 Simplifying quantitative measurement of free radical species using an X-band EPR spectrometer Makino, Yusuke Ueno, Megumi Shoji, Yoshimi Nyui, Minako Nakanishi, Ikuo Fukui, Koji Matsumoto, Ken-ichiro J Clin Biochem Nutr Original Article The quantitative measurement of free radicals in liquid using an X-band electron paramagnetic resonance (EPR) was systematized. Quantification of free radicals by EPR requires a standard sample that contains a known spin amount/concentration. When satisfactory reproducibility of the sample material, volume, shape, and positioning in the cavity for EPR measurements can be guaranteed, a sample tested and a standard can be directly compared and the process of quantification can be simplified. The purpose of this study was to simplify manual quantitative EPR measurement. A suitable sample volume for achieving a stable EPR intensity was estimated. The effects of different solvents on the EPR sensitivity were compared. The stability and reproducibility of the EPR intensity of standard nitroxyl radical solutions were compared among different types of sample tubes. When the sample tubes, sample volumes, and/or solvents were the same, the EPR intensity was reproduced with an error of 2% or less for μM samples. The quantified sample and the standard sample in the same solvent and the same volume drawn into the same sample tube was able to be directly compared. The standard sample for quantification should be measured just before or after every daily experiment. the Society for Free Radical Research Japan 2022-05 2021-12-25 /pmc/articles/PMC9130060/ /pubmed/35692674 http://dx.doi.org/10.3164/jcbn.21-83 Text en Copyright © 2022 JCBN https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Makino, Yusuke
Ueno, Megumi
Shoji, Yoshimi
Nyui, Minako
Nakanishi, Ikuo
Fukui, Koji
Matsumoto, Ken-ichiro
Simplifying quantitative measurement of free radical species using an X-band EPR spectrometer
title Simplifying quantitative measurement of free radical species using an X-band EPR spectrometer
title_full Simplifying quantitative measurement of free radical species using an X-band EPR spectrometer
title_fullStr Simplifying quantitative measurement of free radical species using an X-band EPR spectrometer
title_full_unstemmed Simplifying quantitative measurement of free radical species using an X-band EPR spectrometer
title_short Simplifying quantitative measurement of free radical species using an X-band EPR spectrometer
title_sort simplifying quantitative measurement of free radical species using an x-band epr spectrometer
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130060/
https://www.ncbi.nlm.nih.gov/pubmed/35692674
http://dx.doi.org/10.3164/jcbn.21-83
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