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Molecular mechanisms underlying the promotion of wound repair by coenzyme Q10: PI3K/Akt signal activation via alterations to cell membrane domains

Coenzyme Q10 (CoQ10) promotes wound healing in vitro and in vivo. However, the molecular mechanisms underlying the promoting effects of CoQ10 on wound repair remain unknown. In the present study, we investigated the molecular mechanisms through which CoQ10 induces wound repair using a cellular wound...

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Autores principales: Kurashiki, Tatsuyuki, Horikoshi, Yosuke, Kamizaki, Koki, Sunaguchi, Teppei, Hara, Kazushi, Morimoto, Masaki, Kitagawa, Yoshinori, Nakaso, Kazuhiro, Otsuki, Akihiro, Matsura, Tatsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Society for Free Radical Research Japan 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130066/
https://www.ncbi.nlm.nih.gov/pubmed/35692678
http://dx.doi.org/10.3164/jcbn.21-141
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author Kurashiki, Tatsuyuki
Horikoshi, Yosuke
Kamizaki, Koki
Sunaguchi, Teppei
Hara, Kazushi
Morimoto, Masaki
Kitagawa, Yoshinori
Nakaso, Kazuhiro
Otsuki, Akihiro
Matsura, Tatsuya
author_facet Kurashiki, Tatsuyuki
Horikoshi, Yosuke
Kamizaki, Koki
Sunaguchi, Teppei
Hara, Kazushi
Morimoto, Masaki
Kitagawa, Yoshinori
Nakaso, Kazuhiro
Otsuki, Akihiro
Matsura, Tatsuya
author_sort Kurashiki, Tatsuyuki
collection PubMed
description Coenzyme Q10 (CoQ10) promotes wound healing in vitro and in vivo. However, the molecular mechanisms underlying the promoting effects of CoQ10 on wound repair remain unknown. In the present study, we investigated the molecular mechanisms through which CoQ10 induces wound repair using a cellular wound-healing model. CoQ10 promoted wound closure in a dose-dependent manner and wound-mediated cell polarization after wounding in HaCaT cells. A comparison with other CoQ homologs, benzoquinone derivatives, and polyisoprenyl compounds suggested that the whole structure of CoQ10 is required for potent wound repair. The phosphorylation of Akt after wounding and the plasma membrane translocation of Akt were elevated in CoQ10-treated cells. The promoting effect of CoQ10 on wound repair was abrogated by co-treatment with a phosphatidylinositol 3-kinase (PI3K) inhibitor. Immuno­histochemical and biochemical analyses showed that CoQ10 increased the localization of caveolin-1 (Cav-1) to the apical membrane domains of the cells and the Cav-1 content in the membrane-rich fractions. Depletion of Cav-1 suppressed CoQ10-mediated wound repair and PI3K/Akt signaling activation in HaCaT cells. These results indicated that CoQ10 increases the translocation of Cav-1 to the plasma membranes, activating the downstream PI3K/Akt signaling pathway, and resulting in wound closure in HaCaT cells.
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spelling pubmed-91300662022-06-09 Molecular mechanisms underlying the promotion of wound repair by coenzyme Q10: PI3K/Akt signal activation via alterations to cell membrane domains Kurashiki, Tatsuyuki Horikoshi, Yosuke Kamizaki, Koki Sunaguchi, Teppei Hara, Kazushi Morimoto, Masaki Kitagawa, Yoshinori Nakaso, Kazuhiro Otsuki, Akihiro Matsura, Tatsuya J Clin Biochem Nutr Original Article Coenzyme Q10 (CoQ10) promotes wound healing in vitro and in vivo. However, the molecular mechanisms underlying the promoting effects of CoQ10 on wound repair remain unknown. In the present study, we investigated the molecular mechanisms through which CoQ10 induces wound repair using a cellular wound-healing model. CoQ10 promoted wound closure in a dose-dependent manner and wound-mediated cell polarization after wounding in HaCaT cells. A comparison with other CoQ homologs, benzoquinone derivatives, and polyisoprenyl compounds suggested that the whole structure of CoQ10 is required for potent wound repair. The phosphorylation of Akt after wounding and the plasma membrane translocation of Akt were elevated in CoQ10-treated cells. The promoting effect of CoQ10 on wound repair was abrogated by co-treatment with a phosphatidylinositol 3-kinase (PI3K) inhibitor. Immuno­histochemical and biochemical analyses showed that CoQ10 increased the localization of caveolin-1 (Cav-1) to the apical membrane domains of the cells and the Cav-1 content in the membrane-rich fractions. Depletion of Cav-1 suppressed CoQ10-mediated wound repair and PI3K/Akt signaling activation in HaCaT cells. These results indicated that CoQ10 increases the translocation of Cav-1 to the plasma membranes, activating the downstream PI3K/Akt signaling pathway, and resulting in wound closure in HaCaT cells. the Society for Free Radical Research Japan 2022-05 2022-01-08 /pmc/articles/PMC9130066/ /pubmed/35692678 http://dx.doi.org/10.3164/jcbn.21-141 Text en Copyright © 2022 JCBN https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Kurashiki, Tatsuyuki
Horikoshi, Yosuke
Kamizaki, Koki
Sunaguchi, Teppei
Hara, Kazushi
Morimoto, Masaki
Kitagawa, Yoshinori
Nakaso, Kazuhiro
Otsuki, Akihiro
Matsura, Tatsuya
Molecular mechanisms underlying the promotion of wound repair by coenzyme Q10: PI3K/Akt signal activation via alterations to cell membrane domains
title Molecular mechanisms underlying the promotion of wound repair by coenzyme Q10: PI3K/Akt signal activation via alterations to cell membrane domains
title_full Molecular mechanisms underlying the promotion of wound repair by coenzyme Q10: PI3K/Akt signal activation via alterations to cell membrane domains
title_fullStr Molecular mechanisms underlying the promotion of wound repair by coenzyme Q10: PI3K/Akt signal activation via alterations to cell membrane domains
title_full_unstemmed Molecular mechanisms underlying the promotion of wound repair by coenzyme Q10: PI3K/Akt signal activation via alterations to cell membrane domains
title_short Molecular mechanisms underlying the promotion of wound repair by coenzyme Q10: PI3K/Akt signal activation via alterations to cell membrane domains
title_sort molecular mechanisms underlying the promotion of wound repair by coenzyme q10: pi3k/akt signal activation via alterations to cell membrane domains
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130066/
https://www.ncbi.nlm.nih.gov/pubmed/35692678
http://dx.doi.org/10.3164/jcbn.21-141
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