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Valuable effect of Manuka Honey in increasing the printability and chondrogenic potential of a naturally derived bioink
Hydrogel-based bioinks are the main formulations used for Articular Cartilage (AC) regeneration due to their similarity to chondral tissue in terms of morphological and mechanical properties. However, the main challenge is to design and formulate bioinks able to allow reproducible additive manufactu...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130107/ https://www.ncbi.nlm.nih.gov/pubmed/35647514 http://dx.doi.org/10.1016/j.mtbio.2022.100287 |
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author | Scalzone, Annachiara Cerqueni, Giorgia Bonifacio, Maria A. Pistillo, Michele Cometa, Stefania Belmonte, Monica Mattioli Wang, Xiao N. Dalgarno, Kenny Ferreira, Ana M. De Giglio, Elvira Gentile, Piergiorgio |
author_facet | Scalzone, Annachiara Cerqueni, Giorgia Bonifacio, Maria A. Pistillo, Michele Cometa, Stefania Belmonte, Monica Mattioli Wang, Xiao N. Dalgarno, Kenny Ferreira, Ana M. De Giglio, Elvira Gentile, Piergiorgio |
author_sort | Scalzone, Annachiara |
collection | PubMed |
description | Hydrogel-based bioinks are the main formulations used for Articular Cartilage (AC) regeneration due to their similarity to chondral tissue in terms of morphological and mechanical properties. However, the main challenge is to design and formulate bioinks able to allow reproducible additive manufacturing and fulfil the biological needs for the required tissue. In our work, we investigated an innovative Manuka honey (MH)-loaded photocurable gellan gum methacrylated (GGMA) bioink, encapsulating mesenchymal stem cells differentiated in chondrocytes (MSCs-C), to generate 3D bioprinted construct for AC studies. We demonstrated the beneficial effect of MH incorporation on the bioink printability, leading to the obtainment of a more homogenous filament extrusion and therefore a better printing resolution. Also, GGMA-MH formulation showed higher viscoelastic properties, presenting complex modulus G∗ values of ∼1042 Pa, compared to ∼730 Pa of GGMA. Finally, MH-enriched bioink induced a higher expression of chondrogenic markers col2a1 (14-fold), sox9 (3-fold) and acan (4-fold) and AC ECM main element production (proteoglycans and collagen). |
format | Online Article Text |
id | pubmed-9130107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-91301072022-05-26 Valuable effect of Manuka Honey in increasing the printability and chondrogenic potential of a naturally derived bioink Scalzone, Annachiara Cerqueni, Giorgia Bonifacio, Maria A. Pistillo, Michele Cometa, Stefania Belmonte, Monica Mattioli Wang, Xiao N. Dalgarno, Kenny Ferreira, Ana M. De Giglio, Elvira Gentile, Piergiorgio Mater Today Bio Full Length Article Hydrogel-based bioinks are the main formulations used for Articular Cartilage (AC) regeneration due to their similarity to chondral tissue in terms of morphological and mechanical properties. However, the main challenge is to design and formulate bioinks able to allow reproducible additive manufacturing and fulfil the biological needs for the required tissue. In our work, we investigated an innovative Manuka honey (MH)-loaded photocurable gellan gum methacrylated (GGMA) bioink, encapsulating mesenchymal stem cells differentiated in chondrocytes (MSCs-C), to generate 3D bioprinted construct for AC studies. We demonstrated the beneficial effect of MH incorporation on the bioink printability, leading to the obtainment of a more homogenous filament extrusion and therefore a better printing resolution. Also, GGMA-MH formulation showed higher viscoelastic properties, presenting complex modulus G∗ values of ∼1042 Pa, compared to ∼730 Pa of GGMA. Finally, MH-enriched bioink induced a higher expression of chondrogenic markers col2a1 (14-fold), sox9 (3-fold) and acan (4-fold) and AC ECM main element production (proteoglycans and collagen). Elsevier 2022-05-13 /pmc/articles/PMC9130107/ /pubmed/35647514 http://dx.doi.org/10.1016/j.mtbio.2022.100287 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Full Length Article Scalzone, Annachiara Cerqueni, Giorgia Bonifacio, Maria A. Pistillo, Michele Cometa, Stefania Belmonte, Monica Mattioli Wang, Xiao N. Dalgarno, Kenny Ferreira, Ana M. De Giglio, Elvira Gentile, Piergiorgio Valuable effect of Manuka Honey in increasing the printability and chondrogenic potential of a naturally derived bioink |
title | Valuable effect of Manuka Honey in increasing the printability and chondrogenic potential of a naturally derived bioink |
title_full | Valuable effect of Manuka Honey in increasing the printability and chondrogenic potential of a naturally derived bioink |
title_fullStr | Valuable effect of Manuka Honey in increasing the printability and chondrogenic potential of a naturally derived bioink |
title_full_unstemmed | Valuable effect of Manuka Honey in increasing the printability and chondrogenic potential of a naturally derived bioink |
title_short | Valuable effect of Manuka Honey in increasing the printability and chondrogenic potential of a naturally derived bioink |
title_sort | valuable effect of manuka honey in increasing the printability and chondrogenic potential of a naturally derived bioink |
topic | Full Length Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130107/ https://www.ncbi.nlm.nih.gov/pubmed/35647514 http://dx.doi.org/10.1016/j.mtbio.2022.100287 |
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