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The normal and fibrotic mouse lung classified by spatial proteomic analysis

Single cell classification is elucidating homeostasis and pathology in tissues and whole organs. We applied in situ spatial proteomics by multiplex antibody staining to routinely processed mouse lung, healthy and during a fibrosis model. With a limited validated antibody panel (24) we classify the n...

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Autores principales: Ciccimarra, Roberta, Bolognesi, Maddalena M., Zoboli, Matteo, Cattoretti, Giorgio, Stellari, Franco F., Ravanetti, Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130283/
https://www.ncbi.nlm.nih.gov/pubmed/35610327
http://dx.doi.org/10.1038/s41598-022-12738-9
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author Ciccimarra, Roberta
Bolognesi, Maddalena M.
Zoboli, Matteo
Cattoretti, Giorgio
Stellari, Franco F.
Ravanetti, Francesca
author_facet Ciccimarra, Roberta
Bolognesi, Maddalena M.
Zoboli, Matteo
Cattoretti, Giorgio
Stellari, Franco F.
Ravanetti, Francesca
author_sort Ciccimarra, Roberta
collection PubMed
description Single cell classification is elucidating homeostasis and pathology in tissues and whole organs. We applied in situ spatial proteomics by multiplex antibody staining to routinely processed mouse lung, healthy and during a fibrosis model. With a limited validated antibody panel (24) we classify the normal constituents (alveolar type I and II, bronchial epithelia, endothelial, muscular, stromal and hematopoietic cells) and by quantitative measurements, we show the progress of lung fibrosis over a 4 weeks course, the changing landscape and the cell-specific quantitative variation of a multidrug transporter. An early decline in AT2 alveolar cells and a progressive increase in stromal cells seems at the core of the fibrotic process.
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spelling pubmed-91302832022-05-26 The normal and fibrotic mouse lung classified by spatial proteomic analysis Ciccimarra, Roberta Bolognesi, Maddalena M. Zoboli, Matteo Cattoretti, Giorgio Stellari, Franco F. Ravanetti, Francesca Sci Rep Article Single cell classification is elucidating homeostasis and pathology in tissues and whole organs. We applied in situ spatial proteomics by multiplex antibody staining to routinely processed mouse lung, healthy and during a fibrosis model. With a limited validated antibody panel (24) we classify the normal constituents (alveolar type I and II, bronchial epithelia, endothelial, muscular, stromal and hematopoietic cells) and by quantitative measurements, we show the progress of lung fibrosis over a 4 weeks course, the changing landscape and the cell-specific quantitative variation of a multidrug transporter. An early decline in AT2 alveolar cells and a progressive increase in stromal cells seems at the core of the fibrotic process. Nature Publishing Group UK 2022-05-24 /pmc/articles/PMC9130283/ /pubmed/35610327 http://dx.doi.org/10.1038/s41598-022-12738-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Ciccimarra, Roberta
Bolognesi, Maddalena M.
Zoboli, Matteo
Cattoretti, Giorgio
Stellari, Franco F.
Ravanetti, Francesca
The normal and fibrotic mouse lung classified by spatial proteomic analysis
title The normal and fibrotic mouse lung classified by spatial proteomic analysis
title_full The normal and fibrotic mouse lung classified by spatial proteomic analysis
title_fullStr The normal and fibrotic mouse lung classified by spatial proteomic analysis
title_full_unstemmed The normal and fibrotic mouse lung classified by spatial proteomic analysis
title_short The normal and fibrotic mouse lung classified by spatial proteomic analysis
title_sort normal and fibrotic mouse lung classified by spatial proteomic analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130283/
https://www.ncbi.nlm.nih.gov/pubmed/35610327
http://dx.doi.org/10.1038/s41598-022-12738-9
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