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Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites
Coronavirus disease (COVID-19) is a new and highly contagious disease posing a threat to global public health and wreaking havoc around the world. It's caused by the Coronavirus that causes severe acute respiratory syndrome (SARS-CoV-2). In the current pandemic situation, rapid and accurate SAR...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130314/ https://www.ncbi.nlm.nih.gov/pubmed/35659688 http://dx.doi.org/10.1016/j.bioelechem.2022.108161 |
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author | Hosseini, Morteza Sobhanie, Ebtesam Salehnia, Foad Xu, Guobao Rabbani, Hodjattallah Naghavi Sheikholeslami, Mahsa Firoozbakhtian, Ali Sadeghi, Niloufar Hossein Farajollah, Mohammad Reza Ganjali, Mohammad Vosough, Houman |
author_facet | Hosseini, Morteza Sobhanie, Ebtesam Salehnia, Foad Xu, Guobao Rabbani, Hodjattallah Naghavi Sheikholeslami, Mahsa Firoozbakhtian, Ali Sadeghi, Niloufar Hossein Farajollah, Mohammad Reza Ganjali, Mohammad Vosough, Houman |
author_sort | Hosseini, Morteza |
collection | PubMed |
description | Coronavirus disease (COVID-19) is a new and highly contagious disease posing a threat to global public health and wreaking havoc around the world. It's caused by the Coronavirus that causes severe acute respiratory syndrome (SARS-CoV-2). In the current pandemic situation, rapid and accurate SARS-CoV-2 diagnosis on a large scale is critical for early-stage diagnosis. Early detection and monitoring of viral infections can aid in controlling and preventing infection in large groups of people. Accordingly, we developed a sensitive and high-throughput sandwich electrochemiluminescence immunosensor based on antigen detection for COVID-19 diagnosis (the spike protein of SARS-CoV-2). For the spike protein of SARS-CoV-2, the ECL biosensor had a linear range of 10 ng mL(−1) to 10 µg mL(−1) with a limit of detection of 1.93 ng mL(−1). The sandwich ECL immunosensor could be used in early clinical diagnosis due to its excellent recovery in detecting SARS-CoV-2, rapid analysis (90 min), and ease of use. |
format | Online Article Text |
id | pubmed-9130314 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91303142022-05-25 Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites Hosseini, Morteza Sobhanie, Ebtesam Salehnia, Foad Xu, Guobao Rabbani, Hodjattallah Naghavi Sheikholeslami, Mahsa Firoozbakhtian, Ali Sadeghi, Niloufar Hossein Farajollah, Mohammad Reza Ganjali, Mohammad Vosough, Houman Bioelectrochemistry Article Coronavirus disease (COVID-19) is a new and highly contagious disease posing a threat to global public health and wreaking havoc around the world. It's caused by the Coronavirus that causes severe acute respiratory syndrome (SARS-CoV-2). In the current pandemic situation, rapid and accurate SARS-CoV-2 diagnosis on a large scale is critical for early-stage diagnosis. Early detection and monitoring of viral infections can aid in controlling and preventing infection in large groups of people. Accordingly, we developed a sensitive and high-throughput sandwich electrochemiluminescence immunosensor based on antigen detection for COVID-19 diagnosis (the spike protein of SARS-CoV-2). For the spike protein of SARS-CoV-2, the ECL biosensor had a linear range of 10 ng mL(−1) to 10 µg mL(−1) with a limit of detection of 1.93 ng mL(−1). The sandwich ECL immunosensor could be used in early clinical diagnosis due to its excellent recovery in detecting SARS-CoV-2, rapid analysis (90 min), and ease of use. Elsevier B.V. 2022-10 2022-05-25 /pmc/articles/PMC9130314/ /pubmed/35659688 http://dx.doi.org/10.1016/j.bioelechem.2022.108161 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Hosseini, Morteza Sobhanie, Ebtesam Salehnia, Foad Xu, Guobao Rabbani, Hodjattallah Naghavi Sheikholeslami, Mahsa Firoozbakhtian, Ali Sadeghi, Niloufar Hossein Farajollah, Mohammad Reza Ganjali, Mohammad Vosough, Houman Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites |
title | Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites |
title_full | Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites |
title_fullStr | Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites |
title_full_unstemmed | Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites |
title_short | Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites |
title_sort | development of sandwich electrochemiluminescence immunosensor for covid-19 diagnosis by sars-cov-2 spike protein detection based on au@bsa-luminol nanocomposites |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130314/ https://www.ncbi.nlm.nih.gov/pubmed/35659688 http://dx.doi.org/10.1016/j.bioelechem.2022.108161 |
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