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Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites

Coronavirus disease (COVID-19) is a new and highly contagious disease posing a threat to global public health and wreaking havoc around the world. It's caused by the Coronavirus that causes severe acute respiratory syndrome (SARS-CoV-2). In the current pandemic situation, rapid and accurate SAR...

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Autores principales: Hosseini, Morteza, Sobhanie, Ebtesam, Salehnia, Foad, Xu, Guobao, Rabbani, Hodjattallah, Naghavi Sheikholeslami, Mahsa, Firoozbakhtian, Ali, Sadeghi, Niloufar, Hossein Farajollah, Mohammad, Reza Ganjali, Mohammad, Vosough, Houman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130314/
https://www.ncbi.nlm.nih.gov/pubmed/35659688
http://dx.doi.org/10.1016/j.bioelechem.2022.108161
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author Hosseini, Morteza
Sobhanie, Ebtesam
Salehnia, Foad
Xu, Guobao
Rabbani, Hodjattallah
Naghavi Sheikholeslami, Mahsa
Firoozbakhtian, Ali
Sadeghi, Niloufar
Hossein Farajollah, Mohammad
Reza Ganjali, Mohammad
Vosough, Houman
author_facet Hosseini, Morteza
Sobhanie, Ebtesam
Salehnia, Foad
Xu, Guobao
Rabbani, Hodjattallah
Naghavi Sheikholeslami, Mahsa
Firoozbakhtian, Ali
Sadeghi, Niloufar
Hossein Farajollah, Mohammad
Reza Ganjali, Mohammad
Vosough, Houman
author_sort Hosseini, Morteza
collection PubMed
description Coronavirus disease (COVID-19) is a new and highly contagious disease posing a threat to global public health and wreaking havoc around the world. It's caused by the Coronavirus that causes severe acute respiratory syndrome (SARS-CoV-2). In the current pandemic situation, rapid and accurate SARS-CoV-2 diagnosis on a large scale is critical for early-stage diagnosis. Early detection and monitoring of viral infections can aid in controlling and preventing infection in large groups of people. Accordingly, we developed a sensitive and high-throughput sandwich electrochemiluminescence immunosensor based on antigen detection for COVID-19 diagnosis (the spike protein of SARS-CoV-2). For the spike protein of SARS-CoV-2, the ECL biosensor had a linear range of 10 ng mL(−1) to 10 µg mL(−1) with a limit of detection of 1.93 ng mL(−1). The sandwich ECL immunosensor could be used in early clinical diagnosis due to its excellent recovery in detecting SARS-CoV-2, rapid analysis (90 min), and ease of use.
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spelling pubmed-91303142022-05-25 Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites Hosseini, Morteza Sobhanie, Ebtesam Salehnia, Foad Xu, Guobao Rabbani, Hodjattallah Naghavi Sheikholeslami, Mahsa Firoozbakhtian, Ali Sadeghi, Niloufar Hossein Farajollah, Mohammad Reza Ganjali, Mohammad Vosough, Houman Bioelectrochemistry Article Coronavirus disease (COVID-19) is a new and highly contagious disease posing a threat to global public health and wreaking havoc around the world. It's caused by the Coronavirus that causes severe acute respiratory syndrome (SARS-CoV-2). In the current pandemic situation, rapid and accurate SARS-CoV-2 diagnosis on a large scale is critical for early-stage diagnosis. Early detection and monitoring of viral infections can aid in controlling and preventing infection in large groups of people. Accordingly, we developed a sensitive and high-throughput sandwich electrochemiluminescence immunosensor based on antigen detection for COVID-19 diagnosis (the spike protein of SARS-CoV-2). For the spike protein of SARS-CoV-2, the ECL biosensor had a linear range of 10 ng mL(−1) to 10 µg mL(−1) with a limit of detection of 1.93 ng mL(−1). The sandwich ECL immunosensor could be used in early clinical diagnosis due to its excellent recovery in detecting SARS-CoV-2, rapid analysis (90 min), and ease of use. Elsevier B.V. 2022-10 2022-05-25 /pmc/articles/PMC9130314/ /pubmed/35659688 http://dx.doi.org/10.1016/j.bioelechem.2022.108161 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Hosseini, Morteza
Sobhanie, Ebtesam
Salehnia, Foad
Xu, Guobao
Rabbani, Hodjattallah
Naghavi Sheikholeslami, Mahsa
Firoozbakhtian, Ali
Sadeghi, Niloufar
Hossein Farajollah, Mohammad
Reza Ganjali, Mohammad
Vosough, Houman
Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites
title Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites
title_full Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites
title_fullStr Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites
title_full_unstemmed Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites
title_short Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites
title_sort development of sandwich electrochemiluminescence immunosensor for covid-19 diagnosis by sars-cov-2 spike protein detection based on au@bsa-luminol nanocomposites
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130314/
https://www.ncbi.nlm.nih.gov/pubmed/35659688
http://dx.doi.org/10.1016/j.bioelechem.2022.108161
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