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Structure Insights Into Photosystem I Octamer From Cyanobacteria
The diversity of photosystem oligomers is essential to understanding how photosynthetic organisms adapt to light conditions. Due to its structural and physiological significance, the assembly of the PSI supercomplex has been of great interest recently in terms of both chloroplast and cyanobacteria....
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130954/ https://www.ncbi.nlm.nih.gov/pubmed/35633660 http://dx.doi.org/10.3389/fmicb.2022.876122 |
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author | Chen, Ming He, Yujie Liu, Dongyang Tian, Lijin Xu, Pengqi Liu, Xuan Pan, Yihang Dong, Shuqi He, Jun Zhang, Ying |
author_facet | Chen, Ming He, Yujie Liu, Dongyang Tian, Lijin Xu, Pengqi Liu, Xuan Pan, Yihang Dong, Shuqi He, Jun Zhang, Ying |
author_sort | Chen, Ming |
collection | PubMed |
description | The diversity of photosystem oligomers is essential to understanding how photosynthetic organisms adapt to light conditions. Due to its structural and physiological significance, the assembly of the PSI supercomplex has been of great interest recently in terms of both chloroplast and cyanobacteria. In this study, two novel photosystem I supercomplexes were isolated for the first time from the low light incubated culture of filamentous cyanobacterium Anabaena sp. PCC 7120. These complexes were defined as PSI hexamers and octamers through biochemical and biophysical characterization. Their 77K emission spectra indicated that the red forms of chlorophylls seemed not to be affected during oligomerization. By cryo-EM single-particle analysis, a near-atomic (7.0 Å) resolution structure of a PSI octamer was resolved, and the molecular assemblies of a stable PSI octamer were revealed. |
format | Online Article Text |
id | pubmed-9130954 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91309542022-05-26 Structure Insights Into Photosystem I Octamer From Cyanobacteria Chen, Ming He, Yujie Liu, Dongyang Tian, Lijin Xu, Pengqi Liu, Xuan Pan, Yihang Dong, Shuqi He, Jun Zhang, Ying Front Microbiol Microbiology The diversity of photosystem oligomers is essential to understanding how photosynthetic organisms adapt to light conditions. Due to its structural and physiological significance, the assembly of the PSI supercomplex has been of great interest recently in terms of both chloroplast and cyanobacteria. In this study, two novel photosystem I supercomplexes were isolated for the first time from the low light incubated culture of filamentous cyanobacterium Anabaena sp. PCC 7120. These complexes were defined as PSI hexamers and octamers through biochemical and biophysical characterization. Their 77K emission spectra indicated that the red forms of chlorophylls seemed not to be affected during oligomerization. By cryo-EM single-particle analysis, a near-atomic (7.0 Å) resolution structure of a PSI octamer was resolved, and the molecular assemblies of a stable PSI octamer were revealed. Frontiers Media S.A. 2022-05-06 /pmc/articles/PMC9130954/ /pubmed/35633660 http://dx.doi.org/10.3389/fmicb.2022.876122 Text en Copyright © 2022 Chen, He, Liu, Tian, Xu, Liu, Pan, Dong, He and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Chen, Ming He, Yujie Liu, Dongyang Tian, Lijin Xu, Pengqi Liu, Xuan Pan, Yihang Dong, Shuqi He, Jun Zhang, Ying Structure Insights Into Photosystem I Octamer From Cyanobacteria |
title | Structure Insights Into Photosystem I Octamer From Cyanobacteria |
title_full | Structure Insights Into Photosystem I Octamer From Cyanobacteria |
title_fullStr | Structure Insights Into Photosystem I Octamer From Cyanobacteria |
title_full_unstemmed | Structure Insights Into Photosystem I Octamer From Cyanobacteria |
title_short | Structure Insights Into Photosystem I Octamer From Cyanobacteria |
title_sort | structure insights into photosystem i octamer from cyanobacteria |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130954/ https://www.ncbi.nlm.nih.gov/pubmed/35633660 http://dx.doi.org/10.3389/fmicb.2022.876122 |
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