Cargando…

16S rRNA nanopore sequencing for the diagnosis of ocular infection: a feasibility study

OBJECTIVE: We conducted a feasibility study to verify the effectiveness of 16S ribosomal RNA (rRNA) gene analysis using the nanopore sequencer MinION for identifying causative bacteria in several types of ocular infections. METHODS AND ANALYSIS: Four cases of corneal ulcers, one case of endophthalmi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Omi, Masatoshi, Matsuo, Yoshiyuki, Araki-Sasaki, Kaoru, Oba, Shimpei, Yamada, Haruhiko, Hirota, Kiichi, Takahashi, Kanji
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BMJ Publishing Group 2022
Materias:	Genetics
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131114/ https://www.ncbi.nlm.nih.gov/pubmed/36161861 http://dx.doi.org/10.1136/bmjophth-2021-000910

Ejemplares similares

Successful identification of Granulicatella adiacens in postoperative acute infectious endophthalmitis using a bacterial 16S ribosomal RNA gene-sequencing platform with MinION™: A case report
por: Ishino, Masato, et al.
Publicado: (2022)

Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer
por: Kai, Shinichi, et al.
Publicado: (2019)

Rapid detection of single nucleotide polymorphisms using the MinION nanopore sequencer: a feasibility study for perioperative precision medicine
por: Tabata, Yoshiteru, et al.
Publicado: (2022)

Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution
por: Matsuo, Yoshiyuki, et al.
Publicado: (2021)

Using nanopore sequencing to identify fungi from clinical samples with high phylogenetic resolution
por: Ohta, Atsufumi, et al.
Publicado: (2023)

Aberrant Corneal Homeostasis in Neurosurgery-Induced Neurotrophic Keratopathy
por: Oba, Shimpei, et al.
Publicado: (2022)

Time course of collateral vessel formation after retinal vein occlusion visualized by OCTA and elucidation of factors in their formation
por: Takahashi, Hajime, et al.
Publicado: (2021)

Transmembrane thioredoxin‐related protein TMX1 is reversibly oxidized in response to protein accumulation in the endoplasmic reticulum
por: Matsuo, Yoshiyuki, et al.
Publicado: (2017)

MinION™ Nanopore Sequencing of Skin Microbiome 16S and 16S-23S rRNA Gene Amplicons
por: Rozas, Miquel, et al.
Publicado: (2022)

De novo species identification using 16S rRNA gene nanopore sequencing
por: Angell, Inga Leena, et al.
Publicado: (2020)

Comparison of Illumina versus Nanopore 16S rRNA Gene Sequencing of the Human Nasal Microbiota
por: Heikema, Astrid P., et al.
Publicado: (2020)

Improving the Diagnosis of Bacterial Infections: Evaluation of 16S rRNA Nanopore Metagenomics in Culture-Negative Samples
por: Bouchiat, Coralie, et al.
Publicado: (2022)

SituSeq: an offline protocol for rapid and remote Nanopore 16S rRNA amplicon sequence analysis
por: Zorz, Jackie, et al.
Publicado: (2023)

Development of an efficient antimicrobial susceptibility testing method with species identification by Nanopore sequencing of 16S rRNA amplicons
por: Kawai, Yuto, et al.
Publicado: (2022)

Identification of microbial community in the urban environment: The concordance between conventional culture and nanopore 16S rRNA sequencing
por: Lee, Annie Wing-Tung, et al.
Publicado: (2023)

Microbiota profiling with long amplicons using Nanopore sequencing: full-length 16S rRNA gene and the 16S-ITS-23S of the rrn operon
por: Cuscó, Anna, et al.
Publicado: (2019)

Species-level resolution of 16S rRNA gene amplicons sequenced through the MinION™ portable nanopore sequencer
por: Benítez-Páez, Alfonso, et al.
Publicado: (2016)

NanoRTax, a real-time pipeline for taxonomic and diversity analysis of nanopore 16S rRNA amplicon sequencing data
por: Rodríguez-Pérez, Héctor, et al.
Publicado: (2022)

RESCUE: a validated Nanopore pipeline to classify bacteria through long-read, 16S-ITS-23S rRNA sequencing
por: Petrone, Joseph R., et al.
Publicado: (2023)

Establishment of a novel assessment of the quality of human spermatozoa measuring mitochondrial oxygen metabolism
por: Taniguchi, Hisanori, et al.
Publicado: (2022)

Evaluation of PCR conditions for characterizing bacterial communities with full-length 16S rRNA genes using a portable nanopore sequencer
por: Fujiyoshi, So, et al.
Publicado: (2020)

Synthetic rubber sheet to manage exposure keratopathy
por: Yoshimura, Ayano, et al.
Publicado: (2021)

Corneal ulcers with non-infectious appearance caused by nasolacrimal duct obstruction or canaliculitis
por: Ishimoto, Atsuko, et al.
Publicado: (2022)

16S rRNA Methylase–producing, Gram-Negative Pathogens, Japan
por: Yamane, Kunikazu, et al.
Publicado: (2007)

Campylobacter fetus meningitis confirmed by a 16S rRNA gene analysis using the MinION nanopore sequencer, South Korea, 2016
por: Moon, Jangsup, et al.
Publicado: (2017)

657. Evaluation of Nanopore-Based 16S Ribosomal RNA (rRNA) Gene Sequencing for the Development of a Rapid Infection Intervention Clinical Service
por: Batra, Rahul, et al.
Publicado: (2019)

Volatile anesthetics suppress glucose-stimulated insulin secretion in MIN6 cells by inhibiting glucose-induced activation of hypoxia-inducible factor 1
por: Suzuki, Kengo, et al.
Publicado: (2015)

Development of antitumor biguanides targeting energy metabolism and stress responses in the tumor microenvironment
por: Sakai, Takayuki, et al.
Publicado: (2021)

MinION, a portable long-read sequencer, enables rapid vaginal microbiota analysis in a clinical setting
por: Komiya, Shinnosuke, et al.
Publicado: (2022)

Sevoflurane Does Not Promote the Colony-Forming Ability of Human Mesenchymal Glioblastoma Stem Cells In Vitro
por: Shoji, Tomohiro, et al.
Publicado: (2022)

A preliminary study on the potential of Nanopore MinION and Illumina MiSeq 16S rRNA gene sequencing to characterize building-dust microbiomes
por: Nygaard, Anders B., et al.
Publicado: (2020)

Inflammatory Cytokine-Induced HIF-1 Activation Promotes Epithelial–Mesenchymal Transition in Endometrial Epithelial Cells
por: Hashimoto, Yoshiko, et al.
Publicado: (2023)

Nanopore Sequencing Using the Full-Length 16S rRNA Gene for Detection of Blood-Borne Bacteria in Dogs Reveals a Novel Species of Hemotropic Mycoplasma
por: Huggins, Lucas G., et al.
Publicado: (2022)

Microbiome variations among age classes and diets of captive Asian elephants (Elephas maximus) in Thailand using full-length 16S rRNA nanopore sequencing
por: Klinsawat, Worata, et al.
Publicado: (2023)

Cigarette smoke reversibly activates hypoxia-inducible factor 1 in a reactive oxygen species-dependent manner
por: Daijo, Hiroki, et al.
Publicado: (2016)

Cancerous phenotypes associated with hypoxia-inducible factors are not influenced by the volatile anesthetic isoflurane in renal cell carcinoma
por: Sumi, Chisato, et al.
Publicado: (2019)

Propofol inhibits stromatoxin-1-sensitive voltage-dependent K(+) channels in pancreatic β-cells and enhances insulin secretion
por: Kusunoki, Munenori, et al.
Publicado: (2019)

Pharmacological polysulfide suppresses glucose-stimulated insulin secretion in an ATP-sensitive potassium channel-dependent manner
por: Shoji, Tomohiro, et al.
Publicado: (2019)

Bacterial composition of nasal discharge in children based on highly accurate 16S rRNA gene sequencing analysis
por: Haro, Kaoru, et al.
Publicado: (2020)

Identification of Escherichia coli through analysis of 16S rRNA and 16S-23S rRNA internal transcribed spacer region sequences
por: Magray, Mohammed Siraj Ud Din, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_2hhjtp7tl42q3usj7rouloepje