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A prevalence and molecular characterization of novel pathogenic strains of Macrococcus caseolyticus isolated from external wounds of donkeys in Khartoum State –Sudan

A pathogenic strain of Macrococcus caseolyticus (M. caseolyticus) was isolated from wounds infection during an investigation on donkeys in Khartoum State. (122) samples were collected from external wounds (head, abdomen, back and leg) during different seasons. One isolate (124B) was identified using...

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Autores principales: Ali, Dania E., Allam, Mushal, Altayb, Hisham N., Mursi, D., Adalla, M. A., Mohammed, N. O., Khaier, Mona A. M., Salih, Manal H., Abusalab, Sarah, Abbas, M. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131596/
https://www.ncbi.nlm.nih.gov/pubmed/35614464
http://dx.doi.org/10.1186/s12917-022-03297-2
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author Ali, Dania E.
Allam, Mushal
Altayb, Hisham N.
Mursi, D.
Adalla, M. A.
Mohammed, N. O.
Khaier, Mona A. M.
Salih, Manal H.
Abusalab, Sarah
Abbas, M. A.
author_facet Ali, Dania E.
Allam, Mushal
Altayb, Hisham N.
Mursi, D.
Adalla, M. A.
Mohammed, N. O.
Khaier, Mona A. M.
Salih, Manal H.
Abusalab, Sarah
Abbas, M. A.
author_sort Ali, Dania E.
collection PubMed
description A pathogenic strain of Macrococcus caseolyticus (M. caseolyticus) was isolated from wounds infection during an investigation on donkeys in Khartoum State. (122) samples were collected from external wounds (head, abdomen, back and leg) during different seasons. One isolate (124B) was identified using whole-genome sequence analysis. RAST software identified 31 virulent genes of disease and defense, including methicillin-resistant genes, TatR family and ANT(4′)-Ib. Plasmid rep22 was identified by PlasmidFindet-2.0 Server and a CRISPR. MILST-2.0 predicted many novel alleles. NCBI notated the genome as a novel M. caseolyticus strain (DaniaSudan). The MLST(−)tree(−)V1 revealed that DaniaSudan and KM0211a strains were interrelated. Strain DaniaSudan was resistant to ciprofloxacin, ceftazidime, erythromycin, oxacillin, clindamycin and kanamycin. Mice modeling showed bacteremia and many clinical signs (swelling, allergy, wounds, and hair loss). Enlargement, hyperemia, adhesions and abscesses were observed in many organs. Constructive conclusion The prevalence of the strain was 4.73%, with significant differences between collection seasons and locations of wounds. A highly significant association between doses (10(5) CFU/ml, 10(2) CFU/ml, Intra-peritoneum and sub-cutaneous) and swelling, developing of allergy and loss of hair (p = 0.001, p = 0.000 and p = 0.005) respectively were seen. This result represents the first report of pathogenic strains of M. caseolyticus worldwide. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-022-03297-2.
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spelling pubmed-91315962022-05-26 A prevalence and molecular characterization of novel pathogenic strains of Macrococcus caseolyticus isolated from external wounds of donkeys in Khartoum State –Sudan Ali, Dania E. Allam, Mushal Altayb, Hisham N. Mursi, D. Adalla, M. A. Mohammed, N. O. Khaier, Mona A. M. Salih, Manal H. Abusalab, Sarah Abbas, M. A. BMC Vet Res Research A pathogenic strain of Macrococcus caseolyticus (M. caseolyticus) was isolated from wounds infection during an investigation on donkeys in Khartoum State. (122) samples were collected from external wounds (head, abdomen, back and leg) during different seasons. One isolate (124B) was identified using whole-genome sequence analysis. RAST software identified 31 virulent genes of disease and defense, including methicillin-resistant genes, TatR family and ANT(4′)-Ib. Plasmid rep22 was identified by PlasmidFindet-2.0 Server and a CRISPR. MILST-2.0 predicted many novel alleles. NCBI notated the genome as a novel M. caseolyticus strain (DaniaSudan). The MLST(−)tree(−)V1 revealed that DaniaSudan and KM0211a strains were interrelated. Strain DaniaSudan was resistant to ciprofloxacin, ceftazidime, erythromycin, oxacillin, clindamycin and kanamycin. Mice modeling showed bacteremia and many clinical signs (swelling, allergy, wounds, and hair loss). Enlargement, hyperemia, adhesions and abscesses were observed in many organs. Constructive conclusion The prevalence of the strain was 4.73%, with significant differences between collection seasons and locations of wounds. A highly significant association between doses (10(5) CFU/ml, 10(2) CFU/ml, Intra-peritoneum and sub-cutaneous) and swelling, developing of allergy and loss of hair (p = 0.001, p = 0.000 and p = 0.005) respectively were seen. This result represents the first report of pathogenic strains of M. caseolyticus worldwide. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-022-03297-2. BioMed Central 2022-05-25 /pmc/articles/PMC9131596/ /pubmed/35614464 http://dx.doi.org/10.1186/s12917-022-03297-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Ali, Dania E.
Allam, Mushal
Altayb, Hisham N.
Mursi, D.
Adalla, M. A.
Mohammed, N. O.
Khaier, Mona A. M.
Salih, Manal H.
Abusalab, Sarah
Abbas, M. A.
A prevalence and molecular characterization of novel pathogenic strains of Macrococcus caseolyticus isolated from external wounds of donkeys in Khartoum State –Sudan
title A prevalence and molecular characterization of novel pathogenic strains of Macrococcus caseolyticus isolated from external wounds of donkeys in Khartoum State –Sudan
title_full A prevalence and molecular characterization of novel pathogenic strains of Macrococcus caseolyticus isolated from external wounds of donkeys in Khartoum State –Sudan
title_fullStr A prevalence and molecular characterization of novel pathogenic strains of Macrococcus caseolyticus isolated from external wounds of donkeys in Khartoum State –Sudan
title_full_unstemmed A prevalence and molecular characterization of novel pathogenic strains of Macrococcus caseolyticus isolated from external wounds of donkeys in Khartoum State –Sudan
title_short A prevalence and molecular characterization of novel pathogenic strains of Macrococcus caseolyticus isolated from external wounds of donkeys in Khartoum State –Sudan
title_sort prevalence and molecular characterization of novel pathogenic strains of macrococcus caseolyticus isolated from external wounds of donkeys in khartoum state –sudan
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131596/
https://www.ncbi.nlm.nih.gov/pubmed/35614464
http://dx.doi.org/10.1186/s12917-022-03297-2
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