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Downregulation of ammonium uptake improves the growth and tolerance of Kluyveromyces marxianus at high temperature

The growth and tolerance of Kluyveromyces marxianus at high temperatures decreased significantly in the synthetic medium (SM), which is commonly used in industrial fermentations. After 100 days of adaptive laboratory evolution, a strain named KM234 exhibited excellent tolerance at a high temperature...

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Autores principales: Ai, Yi, Luo, Tongyu, Yu, Yao, Zhou, Jungang, Lu, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131600/
https://www.ncbi.nlm.nih.gov/pubmed/35765191
http://dx.doi.org/10.1002/mbo3.1290
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author Ai, Yi
Luo, Tongyu
Yu, Yao
Zhou, Jungang
Lu, Hong
author_facet Ai, Yi
Luo, Tongyu
Yu, Yao
Zhou, Jungang
Lu, Hong
author_sort Ai, Yi
collection PubMed
description The growth and tolerance of Kluyveromyces marxianus at high temperatures decreased significantly in the synthetic medium (SM), which is commonly used in industrial fermentations. After 100 days of adaptive laboratory evolution, a strain named KM234 exhibited excellent tolerance at a high temperature, without loss of its growth ability at a moderate temperature. Transcriptomic analysis revealed that the KM234 strain decreased the expression of the ammonium (NH(4) (+)) transporter gene MEP3 and increased the synthesis of the amino acid carbon backbone, which may contribute greatly to the high‐temperature growth phenotype. High NH(4) (+) content in SM significantly increased the reactive oxygen species (ROS) production at high temperatures and thus caused toxicity to yeast cells. Replacing NH(4) (+) with organic nitrogen sources or increasing the concentration of potassium ions (K(+)) in the medium restored the growth of the wild‐type K. marxianus at a high temperature in SM. We also showed that the NH(4) (+) toxicity mitigated by K(+) might closely depend on the KIN1 gene. Our results provide a practical solution to industrial fermentation under high‐temperature conditions.
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spelling pubmed-91316002022-05-26 Downregulation of ammonium uptake improves the growth and tolerance of Kluyveromyces marxianus at high temperature Ai, Yi Luo, Tongyu Yu, Yao Zhou, Jungang Lu, Hong Microbiologyopen Original Articles The growth and tolerance of Kluyveromyces marxianus at high temperatures decreased significantly in the synthetic medium (SM), which is commonly used in industrial fermentations. After 100 days of adaptive laboratory evolution, a strain named KM234 exhibited excellent tolerance at a high temperature, without loss of its growth ability at a moderate temperature. Transcriptomic analysis revealed that the KM234 strain decreased the expression of the ammonium (NH(4) (+)) transporter gene MEP3 and increased the synthesis of the amino acid carbon backbone, which may contribute greatly to the high‐temperature growth phenotype. High NH(4) (+) content in SM significantly increased the reactive oxygen species (ROS) production at high temperatures and thus caused toxicity to yeast cells. Replacing NH(4) (+) with organic nitrogen sources or increasing the concentration of potassium ions (K(+)) in the medium restored the growth of the wild‐type K. marxianus at a high temperature in SM. We also showed that the NH(4) (+) toxicity mitigated by K(+) might closely depend on the KIN1 gene. Our results provide a practical solution to industrial fermentation under high‐temperature conditions. John Wiley and Sons Inc. 2022-05-25 /pmc/articles/PMC9131600/ /pubmed/35765191 http://dx.doi.org/10.1002/mbo3.1290 Text en © 2022 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Ai, Yi
Luo, Tongyu
Yu, Yao
Zhou, Jungang
Lu, Hong
Downregulation of ammonium uptake improves the growth and tolerance of Kluyveromyces marxianus at high temperature
title Downregulation of ammonium uptake improves the growth and tolerance of Kluyveromyces marxianus at high temperature
title_full Downregulation of ammonium uptake improves the growth and tolerance of Kluyveromyces marxianus at high temperature
title_fullStr Downregulation of ammonium uptake improves the growth and tolerance of Kluyveromyces marxianus at high temperature
title_full_unstemmed Downregulation of ammonium uptake improves the growth and tolerance of Kluyveromyces marxianus at high temperature
title_short Downregulation of ammonium uptake improves the growth and tolerance of Kluyveromyces marxianus at high temperature
title_sort downregulation of ammonium uptake improves the growth and tolerance of kluyveromyces marxianus at high temperature
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131600/
https://www.ncbi.nlm.nih.gov/pubmed/35765191
http://dx.doi.org/10.1002/mbo3.1290
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