Cargando…

Biological variation in the serum and urine kidney injury markers of a healthy population measured within 24 hours

BACKGROUND AND AIMS: To explore the biological variation (BV) of kidney injury markers in serum and urine of healthy subjects within 24 hours to assist with interpretation of future studies using these biomarkers in the context of known BV. MATERIALS AND METHODS: Serum and urine samples were collect...

Descripción completa

Detalles Bibliográficos
Autores principales: Kong, Li-Rui, Wei, Fei, He, Da-Hai, Zhou, Chao-Qiong, Li, Hong-chuan, Wu, Feng, Luo, Yu, Luo, Jian-wei, Xie, Qian-rong, Peng, Hai, Zhang, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131627/
https://www.ncbi.nlm.nih.gov/pubmed/35610615
http://dx.doi.org/10.1186/s12882-022-02819-2
_version_ 1784713213269508096
author Kong, Li-Rui
Wei, Fei
He, Da-Hai
Zhou, Chao-Qiong
Li, Hong-chuan
Wu, Feng
Luo, Yu
Luo, Jian-wei
Xie, Qian-rong
Peng, Hai
Zhang, Yan
author_facet Kong, Li-Rui
Wei, Fei
He, Da-Hai
Zhou, Chao-Qiong
Li, Hong-chuan
Wu, Feng
Luo, Yu
Luo, Jian-wei
Xie, Qian-rong
Peng, Hai
Zhang, Yan
author_sort Kong, Li-Rui
collection PubMed
description BACKGROUND AND AIMS: To explore the biological variation (BV) of kidney injury markers in serum and urine of healthy subjects within 24 hours to assist with interpretation of future studies using these biomarkers in the context of known BV. MATERIALS AND METHODS: Serum and urine samples were collected every 4 hours (0, 4, 8, 12, 16 and 20 hours) from 31 healthy subjects within 24 hours and serum creatinine (s-Crea), serum β2-microglobin (s-β2MG), serum cystatin C (s-CYSC), serum neutrophil gelatinase-associated lipoprotein (s-NGAL), urine creatinine (u-Crea), urine β2-microglobin (u-β2MG), urine cystatin C (u-CYSC), urine neutrophil gelatinase-associated lipoprotein (u-NGAL) were measured. Outlier and variance homogeneity analyses were performed, followed by CV-ANOVA analysis on trend-corrected data (if relevant), and analytical (CV(A)), within-subject (CV(I)), and between-subject (CV(G)) biological variation were calculated. RESULTS: The concentration of kidney injury markers in male was higher than that in female, except for u-CYSC and u-NGAL. There were no significant difference in serum and urine kidney injury markers concentration at different time points. Serum CV(I) was lower than urine CV(I), serum CV(G) was higher than CV(I), and urine CV(G) was lower than CV(I). The individual index (II) of serum kidney injury markers was less than 0.6, while the II of urinary kidney injury markers was more than 1.0. CONCLUSIONS: This study provides new short-term BV data for kidney injury markers in healthy subjects within 24 hours, which are of great significance in explaining other AKI / CKD studies.
format Online
Article
Text
id pubmed-9131627
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-91316272022-05-26 Biological variation in the serum and urine kidney injury markers of a healthy population measured within 24 hours Kong, Li-Rui Wei, Fei He, Da-Hai Zhou, Chao-Qiong Li, Hong-chuan Wu, Feng Luo, Yu Luo, Jian-wei Xie, Qian-rong Peng, Hai Zhang, Yan BMC Nephrol Research BACKGROUND AND AIMS: To explore the biological variation (BV) of kidney injury markers in serum and urine of healthy subjects within 24 hours to assist with interpretation of future studies using these biomarkers in the context of known BV. MATERIALS AND METHODS: Serum and urine samples were collected every 4 hours (0, 4, 8, 12, 16 and 20 hours) from 31 healthy subjects within 24 hours and serum creatinine (s-Crea), serum β2-microglobin (s-β2MG), serum cystatin C (s-CYSC), serum neutrophil gelatinase-associated lipoprotein (s-NGAL), urine creatinine (u-Crea), urine β2-microglobin (u-β2MG), urine cystatin C (u-CYSC), urine neutrophil gelatinase-associated lipoprotein (u-NGAL) were measured. Outlier and variance homogeneity analyses were performed, followed by CV-ANOVA analysis on trend-corrected data (if relevant), and analytical (CV(A)), within-subject (CV(I)), and between-subject (CV(G)) biological variation were calculated. RESULTS: The concentration of kidney injury markers in male was higher than that in female, except for u-CYSC and u-NGAL. There were no significant difference in serum and urine kidney injury markers concentration at different time points. Serum CV(I) was lower than urine CV(I), serum CV(G) was higher than CV(I), and urine CV(G) was lower than CV(I). The individual index (II) of serum kidney injury markers was less than 0.6, while the II of urinary kidney injury markers was more than 1.0. CONCLUSIONS: This study provides new short-term BV data for kidney injury markers in healthy subjects within 24 hours, which are of great significance in explaining other AKI / CKD studies. BioMed Central 2022-05-24 /pmc/articles/PMC9131627/ /pubmed/35610615 http://dx.doi.org/10.1186/s12882-022-02819-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Kong, Li-Rui
Wei, Fei
He, Da-Hai
Zhou, Chao-Qiong
Li, Hong-chuan
Wu, Feng
Luo, Yu
Luo, Jian-wei
Xie, Qian-rong
Peng, Hai
Zhang, Yan
Biological variation in the serum and urine kidney injury markers of a healthy population measured within 24 hours
title Biological variation in the serum and urine kidney injury markers of a healthy population measured within 24 hours
title_full Biological variation in the serum and urine kidney injury markers of a healthy population measured within 24 hours
title_fullStr Biological variation in the serum and urine kidney injury markers of a healthy population measured within 24 hours
title_full_unstemmed Biological variation in the serum and urine kidney injury markers of a healthy population measured within 24 hours
title_short Biological variation in the serum and urine kidney injury markers of a healthy population measured within 24 hours
title_sort biological variation in the serum and urine kidney injury markers of a healthy population measured within 24 hours
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131627/
https://www.ncbi.nlm.nih.gov/pubmed/35610615
http://dx.doi.org/10.1186/s12882-022-02819-2
work_keys_str_mv AT konglirui biologicalvariationintheserumandurinekidneyinjurymarkersofahealthypopulationmeasuredwithin24hours
AT weifei biologicalvariationintheserumandurinekidneyinjurymarkersofahealthypopulationmeasuredwithin24hours
AT hedahai biologicalvariationintheserumandurinekidneyinjurymarkersofahealthypopulationmeasuredwithin24hours
AT zhouchaoqiong biologicalvariationintheserumandurinekidneyinjurymarkersofahealthypopulationmeasuredwithin24hours
AT lihongchuan biologicalvariationintheserumandurinekidneyinjurymarkersofahealthypopulationmeasuredwithin24hours
AT wufeng biologicalvariationintheserumandurinekidneyinjurymarkersofahealthypopulationmeasuredwithin24hours
AT luoyu biologicalvariationintheserumandurinekidneyinjurymarkersofahealthypopulationmeasuredwithin24hours
AT luojianwei biologicalvariationintheserumandurinekidneyinjurymarkersofahealthypopulationmeasuredwithin24hours
AT xieqianrong biologicalvariationintheserumandurinekidneyinjurymarkersofahealthypopulationmeasuredwithin24hours
AT penghai biologicalvariationintheserumandurinekidneyinjurymarkersofahealthypopulationmeasuredwithin24hours
AT zhangyan biologicalvariationintheserumandurinekidneyinjurymarkersofahealthypopulationmeasuredwithin24hours