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Influence of vascular endothelial growth factor on neutrophil activation in asthmatics

INTRODUCTION: Asthma is a complex syndrome associated with heterogeneity in the type of inflammation that modulates airway hyperresponsiveness and remodelling. Airway inflammation with neutrophils, cytokines like vascular endothelial growth factor (VEGF) and various gene polymorphisms might be relev...

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Autores principales: Gomułka, Krzysztof, Liebhart, Jerzy, Jaskuła, Emilia, Mędrala, Wojciech
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131951/
https://www.ncbi.nlm.nih.gov/pubmed/35645670
http://dx.doi.org/10.5114/ada.2022.115888
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author Gomułka, Krzysztof
Liebhart, Jerzy
Jaskuła, Emilia
Mędrala, Wojciech
author_facet Gomułka, Krzysztof
Liebhart, Jerzy
Jaskuła, Emilia
Mędrala, Wojciech
author_sort Gomułka, Krzysztof
collection PubMed
description INTRODUCTION: Asthma is a complex syndrome associated with heterogeneity in the type of inflammation that modulates airway hyperresponsiveness and remodelling. Airway inflammation with neutrophils, cytokines like vascular endothelial growth factor (VEGF) and various gene polymorphisms might be relevant for asthma pathogenesis. AIM: To investigate the expression of CD69 and CD11b markers on peripheral neutrophils after VEGF in vitro stimulation in asthmatics. Furthermore, the possible influence of a genetic factor (del/ins genotype at -2549 -2567 position in the VEGF-promoter gene) was taken into account. MATERIAL AND METHODS: 122 subjects (82 asthmatics and 40 controls) participated in our study. CD69 and CD11b presence on peripheral blood neutrophils was detected using the flow cytometric method without exogenous stimulation (negative control), after N-formyl-methionine-leucyl-phenylalanine stimulation (fMLP – positive control) and after VEGF stimulation. Genotyping for the VEGF-promoter region was performed by polymerase chain reaction (PCR). RESULTS: Peripheral neutrophil stimulation with VEGF enhances the activity of these cells and induces CD69 and CD11b expression in a dose-dependent manner compared with unstimulated neutrophils (p > 0.05). CD69 and CD11b markers were slightly presented (p = 0.05 and p = 0.07, respectively) on neutrophils stimulated with fMLP in asthmatics with the ins genotype variant in the VEGF-promoter region. CONCLUSIONS: Our results demonstrate that VEGF might insignificantly activate neutrophils in asthmatics. In addition, the modulated expression of CD69 and CD11b on peripheral neutrophils is not related to potential contribution of the VEGF gene polymorphism.
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spelling pubmed-91319512022-05-26 Influence of vascular endothelial growth factor on neutrophil activation in asthmatics Gomułka, Krzysztof Liebhart, Jerzy Jaskuła, Emilia Mędrala, Wojciech Postepy Dermatol Alergol Original Paper INTRODUCTION: Asthma is a complex syndrome associated with heterogeneity in the type of inflammation that modulates airway hyperresponsiveness and remodelling. Airway inflammation with neutrophils, cytokines like vascular endothelial growth factor (VEGF) and various gene polymorphisms might be relevant for asthma pathogenesis. AIM: To investigate the expression of CD69 and CD11b markers on peripheral neutrophils after VEGF in vitro stimulation in asthmatics. Furthermore, the possible influence of a genetic factor (del/ins genotype at -2549 -2567 position in the VEGF-promoter gene) was taken into account. MATERIAL AND METHODS: 122 subjects (82 asthmatics and 40 controls) participated in our study. CD69 and CD11b presence on peripheral blood neutrophils was detected using the flow cytometric method without exogenous stimulation (negative control), after N-formyl-methionine-leucyl-phenylalanine stimulation (fMLP – positive control) and after VEGF stimulation. Genotyping for the VEGF-promoter region was performed by polymerase chain reaction (PCR). RESULTS: Peripheral neutrophil stimulation with VEGF enhances the activity of these cells and induces CD69 and CD11b expression in a dose-dependent manner compared with unstimulated neutrophils (p > 0.05). CD69 and CD11b markers were slightly presented (p = 0.05 and p = 0.07, respectively) on neutrophils stimulated with fMLP in asthmatics with the ins genotype variant in the VEGF-promoter region. CONCLUSIONS: Our results demonstrate that VEGF might insignificantly activate neutrophils in asthmatics. In addition, the modulated expression of CD69 and CD11b on peripheral neutrophils is not related to potential contribution of the VEGF gene polymorphism. Termedia Publishing House 2022-05-09 2022-04 /pmc/articles/PMC9131951/ /pubmed/35645670 http://dx.doi.org/10.5114/ada.2022.115888 Text en Copyright: © 2022 Termedia Sp. z o. o. https://creativecommons.org/licenses/by-nc-sa/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
spellingShingle Original Paper
Gomułka, Krzysztof
Liebhart, Jerzy
Jaskuła, Emilia
Mędrala, Wojciech
Influence of vascular endothelial growth factor on neutrophil activation in asthmatics
title Influence of vascular endothelial growth factor on neutrophil activation in asthmatics
title_full Influence of vascular endothelial growth factor on neutrophil activation in asthmatics
title_fullStr Influence of vascular endothelial growth factor on neutrophil activation in asthmatics
title_full_unstemmed Influence of vascular endothelial growth factor on neutrophil activation in asthmatics
title_short Influence of vascular endothelial growth factor on neutrophil activation in asthmatics
title_sort influence of vascular endothelial growth factor on neutrophil activation in asthmatics
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131951/
https://www.ncbi.nlm.nih.gov/pubmed/35645670
http://dx.doi.org/10.5114/ada.2022.115888
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