Description of Corynebacterium poyangense sp. nov., isolated from the feces of the greater white-fronted geese (Anser albifrons)

Two novel Gram-positive, non-spore-forming, facultatively anaerobic, non-motile, and short rods to coccoid strains were isolated from the feces of the greater white-fronted geese (Anser albifrons) at Poyang Lake. The 16S rRNA gene sequences of strains 4H37-19(T) and 3HC-13 shared highest identity to that of Corynebacterium uropygiale Iso10(T) (97.8%). Phylogenetic and phylogenomic analyses indicated that strains 4H37-19(T) and 3HC-13 formed an independent clade within genus Corynebacterium and clustered with Corynebacterium uropygiale Iso10(T). The average nucleotide identity and digital DNA-DNA hybridization value between strains 4H37-19(T) and 3HC-13 and members within genus Corynebacterium were all below 95% and 70%, respectively. The genomic G + C content of strains 4H37-19(T) and 3HC-13 was 52.5%. Diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylcholine, and phosphatidyl inositol mannosides (PIM) were the major polar lipids, with C(18:1)ω9c, C(16:0), and C(18:0) as the major fatty acids, and MK-8 (H4), MK-8(H(2)), and MK-9(H(2)) as the predominant respiratory quinones. The major whole cell sugar was arabinose, and the cell wall included mycolic acids. The cell wall peptidoglycan contained meso-diaminopimelic acid (meso-DAP). The polyphasic taxonomic data shows that these two strains represent a novel species of the genus Corynebacterium, for which the name Corynebacterium poyangense sp. nov. is proposed. The type strain of Corynebacterium poyangense is 4H37-19(T) (=GDMCC 1.1738(T) = KACC 21671(T)). Supplemental material for this article may be found at 10.1007/s12275-022-2089-9.