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NF-YA transcription factors suppress jasmonic acid-mediated antiviral defense and facilitate viral infection in rice
NF-Y transcription factors are known to play many diverse roles in the development and physiological responses of plants but little is known about their role in plant defense. Here, we demonstrate the negative roles of rice NF-YA family genes in antiviral defense against two different plant viruses,...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132283/ https://www.ncbi.nlm.nih.gov/pubmed/35560151 http://dx.doi.org/10.1371/journal.ppat.1010548 |
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author | Tan, Xiaoxiang Zhang, Hehong Yang, Zihang Wei, Zhongyan Li, Yanjun Chen, Jianping Sun, Zongtao |
author_facet | Tan, Xiaoxiang Zhang, Hehong Yang, Zihang Wei, Zhongyan Li, Yanjun Chen, Jianping Sun, Zongtao |
author_sort | Tan, Xiaoxiang |
collection | PubMed |
description | NF-Y transcription factors are known to play many diverse roles in the development and physiological responses of plants but little is known about their role in plant defense. Here, we demonstrate the negative roles of rice NF-YA family genes in antiviral defense against two different plant viruses, Rice stripe virus (RSV, Tenuivirus) and Southern rice black-streaked dwarf virus (SRBSDV, Fijivirus). RSV and SRBSDV both induced the expression of OsNF-YA family genes. Overexpression of OsNF-YAs enhanced rice susceptibility to virus infection, while OsNF-YAs RNAi mutants were more resistant. Transcriptome sequencing showed that the expression of jasmonic acid (JA)-related genes was significantly decreased in plants overexpressing OsNF-YA when they were infected by viruses. qRT-PCR and JA sensitivity assays confirmed that OsNF-YAs play negative roles in regulating the JA pathway. Further experiments showed that OsNF-YAs physically interact with JA signaling transcription factors OsMYC2/3 and interfere with JA signaling by dissociating the OsMYC2/3-OsMED25 complex, which inhibits the transcriptional activation activity of OsMYC2/3. Together, our results reveal that OsNF-YAs broadly inhibit plant antiviral defense by repressing JA signaling pathways, and provide new insight into how OsNF-YAs are directly associated with the JA pathway. |
format | Online Article Text |
id | pubmed-9132283 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-91322832022-05-26 NF-YA transcription factors suppress jasmonic acid-mediated antiviral defense and facilitate viral infection in rice Tan, Xiaoxiang Zhang, Hehong Yang, Zihang Wei, Zhongyan Li, Yanjun Chen, Jianping Sun, Zongtao PLoS Pathog Research Article NF-Y transcription factors are known to play many diverse roles in the development and physiological responses of plants but little is known about their role in plant defense. Here, we demonstrate the negative roles of rice NF-YA family genes in antiviral defense against two different plant viruses, Rice stripe virus (RSV, Tenuivirus) and Southern rice black-streaked dwarf virus (SRBSDV, Fijivirus). RSV and SRBSDV both induced the expression of OsNF-YA family genes. Overexpression of OsNF-YAs enhanced rice susceptibility to virus infection, while OsNF-YAs RNAi mutants were more resistant. Transcriptome sequencing showed that the expression of jasmonic acid (JA)-related genes was significantly decreased in plants overexpressing OsNF-YA when they were infected by viruses. qRT-PCR and JA sensitivity assays confirmed that OsNF-YAs play negative roles in regulating the JA pathway. Further experiments showed that OsNF-YAs physically interact with JA signaling transcription factors OsMYC2/3 and interfere with JA signaling by dissociating the OsMYC2/3-OsMED25 complex, which inhibits the transcriptional activation activity of OsMYC2/3. Together, our results reveal that OsNF-YAs broadly inhibit plant antiviral defense by repressing JA signaling pathways, and provide new insight into how OsNF-YAs are directly associated with the JA pathway. Public Library of Science 2022-05-13 /pmc/articles/PMC9132283/ /pubmed/35560151 http://dx.doi.org/10.1371/journal.ppat.1010548 Text en © 2022 Tan et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Tan, Xiaoxiang Zhang, Hehong Yang, Zihang Wei, Zhongyan Li, Yanjun Chen, Jianping Sun, Zongtao NF-YA transcription factors suppress jasmonic acid-mediated antiviral defense and facilitate viral infection in rice |
title | NF-YA transcription factors suppress jasmonic acid-mediated antiviral defense and facilitate viral infection in rice |
title_full | NF-YA transcription factors suppress jasmonic acid-mediated antiviral defense and facilitate viral infection in rice |
title_fullStr | NF-YA transcription factors suppress jasmonic acid-mediated antiviral defense and facilitate viral infection in rice |
title_full_unstemmed | NF-YA transcription factors suppress jasmonic acid-mediated antiviral defense and facilitate viral infection in rice |
title_short | NF-YA transcription factors suppress jasmonic acid-mediated antiviral defense and facilitate viral infection in rice |
title_sort | nf-ya transcription factors suppress jasmonic acid-mediated antiviral defense and facilitate viral infection in rice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132283/ https://www.ncbi.nlm.nih.gov/pubmed/35560151 http://dx.doi.org/10.1371/journal.ppat.1010548 |
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