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Serial histological changes in the cartilaginous eustachian tube in the rat following balloon dilation

Although balloon dilation has shown promising results in the treatment of dilatory Eustachian tube (ET) dysfunction, the histological effects of ET balloon dilation (ETBD) is unknown because histological examination of the whole human cartilaginous ET is impossible. Animal studies are needed to eluc...

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Autores principales: Kim, Yehree, Kang, Jeon Min, Ryu, Dae Sung, Park, Jung-Hoon, Kang, Woo Seok, Park, Hong Ju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132338/
https://www.ncbi.nlm.nih.gov/pubmed/35613135
http://dx.doi.org/10.1371/journal.pone.0268763
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author Kim, Yehree
Kang, Jeon Min
Ryu, Dae Sung
Park, Jung-Hoon
Kang, Woo Seok
Park, Hong Ju
author_facet Kim, Yehree
Kang, Jeon Min
Ryu, Dae Sung
Park, Jung-Hoon
Kang, Woo Seok
Park, Hong Ju
author_sort Kim, Yehree
collection PubMed
description Although balloon dilation has shown promising results in the treatment of dilatory Eustachian tube (ET) dysfunction, the histological effects of ET balloon dilation (ETBD) is unknown because histological examination of the whole human cartilaginous ET is impossible. Animal studies are needed to elucidate the effect of ETBD so we evaluated the histological changes after ETBD in a rat model. The left ET of 20 Wistar rats was dilated with a balloon catheter and the right ET was used as a control. Five rats were sacrificed immediately after ETBD, at 1, 4 and 12 weeks after the procedure for histological examination. The epithelial cells, presence of epithelial hyperplasia, and the proportion of the goblet cells in the epithelium; the vascular structures and dimensions of the submucosa; and presence of cartilage fracture and the area of the ET lumen were evaluated and compared between the groups. Desquamation of nearly all epithelial cells and the fracture of tubal cartilages were observed immediately after ETBD. At 1-week post-ETBD, the ciliated epithelial cells started to recover with epithelial hyperplasia. The goblet cells recovered by 4 weeks post-ETBD and epithelial hyperplasia decreased but was still present at 12 weeks post-ETBD. The depth of the submucosa increased and neovascularization in this region was observed at 1-week post-ETBD and persisted up to 12 weeks post-ETBD. The lumen of the cartilaginous ET increased immediately after ETBD but decreased at 1-week post-ETBD. The cartilaginous ET lumen recovered to the normal value at 4 weeks post-ETBD. This study is the first to describe the serial histological changes to the cartilaginous ET after ETBD and helps our understanding of the histological changes that occur after an ETBD intervention for intractable ET dysfunction.
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spelling pubmed-91323382022-05-26 Serial histological changes in the cartilaginous eustachian tube in the rat following balloon dilation Kim, Yehree Kang, Jeon Min Ryu, Dae Sung Park, Jung-Hoon Kang, Woo Seok Park, Hong Ju PLoS One Research Article Although balloon dilation has shown promising results in the treatment of dilatory Eustachian tube (ET) dysfunction, the histological effects of ET balloon dilation (ETBD) is unknown because histological examination of the whole human cartilaginous ET is impossible. Animal studies are needed to elucidate the effect of ETBD so we evaluated the histological changes after ETBD in a rat model. The left ET of 20 Wistar rats was dilated with a balloon catheter and the right ET was used as a control. Five rats were sacrificed immediately after ETBD, at 1, 4 and 12 weeks after the procedure for histological examination. The epithelial cells, presence of epithelial hyperplasia, and the proportion of the goblet cells in the epithelium; the vascular structures and dimensions of the submucosa; and presence of cartilage fracture and the area of the ET lumen were evaluated and compared between the groups. Desquamation of nearly all epithelial cells and the fracture of tubal cartilages were observed immediately after ETBD. At 1-week post-ETBD, the ciliated epithelial cells started to recover with epithelial hyperplasia. The goblet cells recovered by 4 weeks post-ETBD and epithelial hyperplasia decreased but was still present at 12 weeks post-ETBD. The depth of the submucosa increased and neovascularization in this region was observed at 1-week post-ETBD and persisted up to 12 weeks post-ETBD. The lumen of the cartilaginous ET increased immediately after ETBD but decreased at 1-week post-ETBD. The cartilaginous ET lumen recovered to the normal value at 4 weeks post-ETBD. This study is the first to describe the serial histological changes to the cartilaginous ET after ETBD and helps our understanding of the histological changes that occur after an ETBD intervention for intractable ET dysfunction. Public Library of Science 2022-05-25 /pmc/articles/PMC9132338/ /pubmed/35613135 http://dx.doi.org/10.1371/journal.pone.0268763 Text en © 2022 Kim et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kim, Yehree
Kang, Jeon Min
Ryu, Dae Sung
Park, Jung-Hoon
Kang, Woo Seok
Park, Hong Ju
Serial histological changes in the cartilaginous eustachian tube in the rat following balloon dilation
title Serial histological changes in the cartilaginous eustachian tube in the rat following balloon dilation
title_full Serial histological changes in the cartilaginous eustachian tube in the rat following balloon dilation
title_fullStr Serial histological changes in the cartilaginous eustachian tube in the rat following balloon dilation
title_full_unstemmed Serial histological changes in the cartilaginous eustachian tube in the rat following balloon dilation
title_short Serial histological changes in the cartilaginous eustachian tube in the rat following balloon dilation
title_sort serial histological changes in the cartilaginous eustachian tube in the rat following balloon dilation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132338/
https://www.ncbi.nlm.nih.gov/pubmed/35613135
http://dx.doi.org/10.1371/journal.pone.0268763
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