Chitinolytic and Antagonistic Activity of Streptomyces Isolated from Fadama Soil against Phytopathogenic Fungi

Chitinases which degrade chitin have attracted attention as biological antifungal agents. The purpose of this study is to isolate Streptomyces from Fadama soil and assess its chitinolytic and antagonist potential against phytopathogenic fungi for application as biocontrol agent. Streptomyces were is...

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Autores principales: Umar, Aminu Argungu, Hussaini, Aminu Bandam, Yahayya, Jibril, Sani, Ibrahim, Aminu, Habiba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Penerbit Universiti Sains Malaysia 2021
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Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132557/
https://www.ncbi.nlm.nih.gov/pubmed/35656363
http://dx.doi.org/10.21315/tlsr2021.32.3.2
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author Umar, Aminu Argungu
Hussaini, Aminu Bandam
Yahayya, Jibril
Sani, Ibrahim
Aminu, Habiba
author_facet Umar, Aminu Argungu
Hussaini, Aminu Bandam
Yahayya, Jibril
Sani, Ibrahim
Aminu, Habiba
author_sort Umar, Aminu Argungu
collection PubMed
description Chitinases which degrade chitin have attracted attention as biological antifungal agents. The purpose of this study is to isolate Streptomyces from Fadama soil and assess its chitinolytic and antagonist potential against phytopathogenic fungi for application as biocontrol agent. Streptomyces were isolated from Fadama soil. The selected isolate CT02 exhibited chitinolytic characteristics. Chitinase production was performed under different temperatures, pH and varying incubation period. The highest chitinase production by CT02 isolate was observed after five days of cultivation. The highest chitinase activity was observed at 35°C and pH 7. The crude extracellular enzyme exhibited a specific activity of 4.20 U/μg whereas partially purified extracellular enzyme exhibited a specific activity of 6.19 U/μg with purification fold of 1.47. The selected isolate CT02 and its extracellular crude chitinase showed in vitro antifungal antagonist potential by inhibiting the growth of Aspergillus niger and Aspergillus oryzae. This indicates that Streptomyces derived chitinases are potential biocontrol agents against phytopathogenic fungi.
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spelling pubmed-91325572022-06-01 Chitinolytic and Antagonistic Activity of Streptomyces Isolated from Fadama Soil against Phytopathogenic Fungi Umar, Aminu Argungu Hussaini, Aminu Bandam Yahayya, Jibril Sani, Ibrahim Aminu, Habiba Trop Life Sci Res Articles Chitinases which degrade chitin have attracted attention as biological antifungal agents. The purpose of this study is to isolate Streptomyces from Fadama soil and assess its chitinolytic and antagonist potential against phytopathogenic fungi for application as biocontrol agent. Streptomyces were isolated from Fadama soil. The selected isolate CT02 exhibited chitinolytic characteristics. Chitinase production was performed under different temperatures, pH and varying incubation period. The highest chitinase production by CT02 isolate was observed after five days of cultivation. The highest chitinase activity was observed at 35°C and pH 7. The crude extracellular enzyme exhibited a specific activity of 4.20 U/μg whereas partially purified extracellular enzyme exhibited a specific activity of 6.19 U/μg with purification fold of 1.47. The selected isolate CT02 and its extracellular crude chitinase showed in vitro antifungal antagonist potential by inhibiting the growth of Aspergillus niger and Aspergillus oryzae. This indicates that Streptomyces derived chitinases are potential biocontrol agents against phytopathogenic fungi. Penerbit Universiti Sains Malaysia 2021-09 2021-09-30 /pmc/articles/PMC9132557/ /pubmed/35656363 http://dx.doi.org/10.21315/tlsr2021.32.3.2 Text en © Penerbit Universiti Sains Malaysia, 2021 https://creativecommons.org/licenses/by/4.0/This work is licensed under the terms of the Creative Commons Attribution (CC BY) (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Articles
Umar, Aminu Argungu
Hussaini, Aminu Bandam
Yahayya, Jibril
Sani, Ibrahim
Aminu, Habiba
Chitinolytic and Antagonistic Activity of Streptomyces Isolated from Fadama Soil against Phytopathogenic Fungi
title Chitinolytic and Antagonistic Activity of Streptomyces Isolated from Fadama Soil against Phytopathogenic Fungi
title_full Chitinolytic and Antagonistic Activity of Streptomyces Isolated from Fadama Soil against Phytopathogenic Fungi
title_fullStr Chitinolytic and Antagonistic Activity of Streptomyces Isolated from Fadama Soil against Phytopathogenic Fungi
title_full_unstemmed Chitinolytic and Antagonistic Activity of Streptomyces Isolated from Fadama Soil against Phytopathogenic Fungi
title_short Chitinolytic and Antagonistic Activity of Streptomyces Isolated from Fadama Soil against Phytopathogenic Fungi
title_sort chitinolytic and antagonistic activity of streptomyces isolated from fadama soil against phytopathogenic fungi
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132557/
https://www.ncbi.nlm.nih.gov/pubmed/35656363
http://dx.doi.org/10.21315/tlsr2021.32.3.2
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