Cargando…

Post-mortem Computed Tomographic Angiography in Equine Distal Forelimbs: A Feasibility Study

In-depth understanding of pathophysiological processes occurring in the vasculature of the equine distal limb is of great importance to improve both diagnostic and therapeutic approaches to diseases. To gain further insights, a model allowing high-resolution 3D-visualization of the vasculature is ne...

Descripción completa

Detalles Bibliográficos
Autores principales: Blaettler, Chantal, Kaessmeyer, Sabine, Grabherr, Silke, Koch, Christoph, Schweizer, Daniela, Van der Vekens, Elke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132589/
https://www.ncbi.nlm.nih.gov/pubmed/35647108
http://dx.doi.org/10.3389/fvets.2022.868390
_version_ 1784713412680351744
author Blaettler, Chantal
Kaessmeyer, Sabine
Grabherr, Silke
Koch, Christoph
Schweizer, Daniela
Van der Vekens, Elke
author_facet Blaettler, Chantal
Kaessmeyer, Sabine
Grabherr, Silke
Koch, Christoph
Schweizer, Daniela
Van der Vekens, Elke
author_sort Blaettler, Chantal
collection PubMed
description In-depth understanding of pathophysiological processes occurring in the vasculature of the equine distal limb is of great importance to improve both diagnostic and therapeutic approaches to diseases. To gain further insights, a model allowing high-resolution 3D-visualization of the vasculature is necessary. This pilot study evaluated the feasibility of restoring vascular perfusion in frozen-thawed distal equine cadaver limbs without prior preparation using computer tomographic imaging (CT). Five frozen-thawed, radiographically normal forelimbs were perfused with a lipophilic contrast agent through the median artery and radial vein in three phases (arterial, venous, and arterial-venous combined (AVC) dynamic). For comparison, one additional limb was perfused with a hydrosoluble contrast agent. The CT-studies (16-slice MDCT, 140 kV, 200 mA, 2 mm slice thickness, 1 mm increment, pitch 0.688) were evaluated at 11 specified regions for visualization of the vasculature and presence of artifacts or anatomic variations. The protocol used in this study proved to be feasible and provided good visualization (93.1%) of vasculature with low rates of artifacts. During the different phases, vascular visualization was similar, but while filling defects decreased in the later phases, extravasation worsened in the 2 limbs where it was observed. Subjectively, the best quality of angiographic images was achieved during the AVC dynamic phase. Perfusion with hydrosoluble contrast resulted in significantly lower vascular visualization (74.0%) and higher artifact rates. This study shows that reperfusion of frozen-thawed equine distal limbs with a lipophilic contrast agent allows for high-quality 3D-visualization of the vasculature and may serve as a model for in situ vascular evaluation in the future.
format Online
Article
Text
id pubmed-9132589
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-91325892022-05-26 Post-mortem Computed Tomographic Angiography in Equine Distal Forelimbs: A Feasibility Study Blaettler, Chantal Kaessmeyer, Sabine Grabherr, Silke Koch, Christoph Schweizer, Daniela Van der Vekens, Elke Front Vet Sci Veterinary Science In-depth understanding of pathophysiological processes occurring in the vasculature of the equine distal limb is of great importance to improve both diagnostic and therapeutic approaches to diseases. To gain further insights, a model allowing high-resolution 3D-visualization of the vasculature is necessary. This pilot study evaluated the feasibility of restoring vascular perfusion in frozen-thawed distal equine cadaver limbs without prior preparation using computer tomographic imaging (CT). Five frozen-thawed, radiographically normal forelimbs were perfused with a lipophilic contrast agent through the median artery and radial vein in three phases (arterial, venous, and arterial-venous combined (AVC) dynamic). For comparison, one additional limb was perfused with a hydrosoluble contrast agent. The CT-studies (16-slice MDCT, 140 kV, 200 mA, 2 mm slice thickness, 1 mm increment, pitch 0.688) were evaluated at 11 specified regions for visualization of the vasculature and presence of artifacts or anatomic variations. The protocol used in this study proved to be feasible and provided good visualization (93.1%) of vasculature with low rates of artifacts. During the different phases, vascular visualization was similar, but while filling defects decreased in the later phases, extravasation worsened in the 2 limbs where it was observed. Subjectively, the best quality of angiographic images was achieved during the AVC dynamic phase. Perfusion with hydrosoluble contrast resulted in significantly lower vascular visualization (74.0%) and higher artifact rates. This study shows that reperfusion of frozen-thawed equine distal limbs with a lipophilic contrast agent allows for high-quality 3D-visualization of the vasculature and may serve as a model for in situ vascular evaluation in the future. Frontiers Media S.A. 2022-05-11 /pmc/articles/PMC9132589/ /pubmed/35647108 http://dx.doi.org/10.3389/fvets.2022.868390 Text en Copyright © 2022 Blaettler, Kaessmeyer, Grabherr, Koch, Schweizer and Van der Vekens. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Blaettler, Chantal
Kaessmeyer, Sabine
Grabherr, Silke
Koch, Christoph
Schweizer, Daniela
Van der Vekens, Elke
Post-mortem Computed Tomographic Angiography in Equine Distal Forelimbs: A Feasibility Study
title Post-mortem Computed Tomographic Angiography in Equine Distal Forelimbs: A Feasibility Study
title_full Post-mortem Computed Tomographic Angiography in Equine Distal Forelimbs: A Feasibility Study
title_fullStr Post-mortem Computed Tomographic Angiography in Equine Distal Forelimbs: A Feasibility Study
title_full_unstemmed Post-mortem Computed Tomographic Angiography in Equine Distal Forelimbs: A Feasibility Study
title_short Post-mortem Computed Tomographic Angiography in Equine Distal Forelimbs: A Feasibility Study
title_sort post-mortem computed tomographic angiography in equine distal forelimbs: a feasibility study
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132589/
https://www.ncbi.nlm.nih.gov/pubmed/35647108
http://dx.doi.org/10.3389/fvets.2022.868390
work_keys_str_mv AT blaettlerchantal postmortemcomputedtomographicangiographyinequinedistalforelimbsafeasibilitystudy
AT kaessmeyersabine postmortemcomputedtomographicangiographyinequinedistalforelimbsafeasibilitystudy
AT grabherrsilke postmortemcomputedtomographicangiographyinequinedistalforelimbsafeasibilitystudy
AT kochchristoph postmortemcomputedtomographicangiographyinequinedistalforelimbsafeasibilitystudy
AT schweizerdaniela postmortemcomputedtomographicangiographyinequinedistalforelimbsafeasibilitystudy
AT vandervekenselke postmortemcomputedtomographicangiographyinequinedistalforelimbsafeasibilitystudy