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Histopathological Investigations and Molecular Confirmation Reveal Mycobacterium bovis in One-Horned Rhinoceros (Rhinoceros unicorns)

Mycobacterium bovis causes tuberculosis in dairy and wild animals. Presence of tuberculosis in animals poses a threat not only to their herd mates but also for public. No reports are available about the clinical, pathological, and molecular investigation of naturally occurring tuberculosis (TB) due...

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Autores principales: Siddique, Abu Baker, Hussain, Riaz, Jamal, Adil, Hossain, Md. Belal, Ahmad, Zulfiqar, Mansoor, Muhammad Khalid, Khan, Iahtasham, Zahra, Kainat, Khan, Ahrar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132650/
https://www.ncbi.nlm.nih.gov/pubmed/35647178
http://dx.doi.org/10.1155/2022/5816986
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author Siddique, Abu Baker
Hussain, Riaz
Jamal, Adil
Hossain, Md. Belal
Ahmad, Zulfiqar
Mansoor, Muhammad Khalid
Khan, Iahtasham
Zahra, Kainat
Khan, Ahrar
author_facet Siddique, Abu Baker
Hussain, Riaz
Jamal, Adil
Hossain, Md. Belal
Ahmad, Zulfiqar
Mansoor, Muhammad Khalid
Khan, Iahtasham
Zahra, Kainat
Khan, Ahrar
author_sort Siddique, Abu Baker
collection PubMed
description Mycobacterium bovis causes tuberculosis in dairy and wild animals. Presence of tuberculosis in animals poses a threat not only to their herd mates but also for public. No reports are available about the clinical, pathological, and molecular investigation of naturally occurring tuberculosis (TB) due to M. bovis in one-horned rhinoceros. One-horned female rhinoceros (Rhinoceros unicorns) at the age of 41 years died in a public park in Pakistan. Postmortem and other investigations were carried out to know the cause of death. The present study describes necropsy, histopathology, and molecular-based confirmation of TB in a captive female rhinoceros that died of this infection. Clinically, the rhinoceros showed nonspecific clinical signs including anorexia, lethargy, dyspnoea, coughing, and sudden death. At necropsy, the trachea exhibited mild congestion and contained catarrhal exudate at the bronchial bifurcation. Macroscopic examination revealed characteristic tubercles on all parenchymatous organs. The lungs showed consolidation, grey hepatization, and contained granulomatous lesions packed with cheesy exudate. Histopathological examination showed severe pneumonic changes in the form of granulomatous inflammation consisting of lymphocytes, multinucleated giant cells, caseous materials, and mineralized foci surrounded by a fibrous capsule. PCR amplicon of 500 bp confirmed the presence of M. bovis in multiple hepatic and pulmonary tissue samples, as well as in uterine exudates. It was concluded that the presence of tuberculosis in rhinoceros may pose potential transmission risk to other animals and the application of practical tools to determine TB status in the rhinoceros is crucial.
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spelling pubmed-91326502022-05-26 Histopathological Investigations and Molecular Confirmation Reveal Mycobacterium bovis in One-Horned Rhinoceros (Rhinoceros unicorns) Siddique, Abu Baker Hussain, Riaz Jamal, Adil Hossain, Md. Belal Ahmad, Zulfiqar Mansoor, Muhammad Khalid Khan, Iahtasham Zahra, Kainat Khan, Ahrar Biomed Res Int Research Article Mycobacterium bovis causes tuberculosis in dairy and wild animals. Presence of tuberculosis in animals poses a threat not only to their herd mates but also for public. No reports are available about the clinical, pathological, and molecular investigation of naturally occurring tuberculosis (TB) due to M. bovis in one-horned rhinoceros. One-horned female rhinoceros (Rhinoceros unicorns) at the age of 41 years died in a public park in Pakistan. Postmortem and other investigations were carried out to know the cause of death. The present study describes necropsy, histopathology, and molecular-based confirmation of TB in a captive female rhinoceros that died of this infection. Clinically, the rhinoceros showed nonspecific clinical signs including anorexia, lethargy, dyspnoea, coughing, and sudden death. At necropsy, the trachea exhibited mild congestion and contained catarrhal exudate at the bronchial bifurcation. Macroscopic examination revealed characteristic tubercles on all parenchymatous organs. The lungs showed consolidation, grey hepatization, and contained granulomatous lesions packed with cheesy exudate. Histopathological examination showed severe pneumonic changes in the form of granulomatous inflammation consisting of lymphocytes, multinucleated giant cells, caseous materials, and mineralized foci surrounded by a fibrous capsule. PCR amplicon of 500 bp confirmed the presence of M. bovis in multiple hepatic and pulmonary tissue samples, as well as in uterine exudates. It was concluded that the presence of tuberculosis in rhinoceros may pose potential transmission risk to other animals and the application of practical tools to determine TB status in the rhinoceros is crucial. Hindawi 2022-05-18 /pmc/articles/PMC9132650/ /pubmed/35647178 http://dx.doi.org/10.1155/2022/5816986 Text en Copyright © 2022 Abu Baker Siddique et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Siddique, Abu Baker
Hussain, Riaz
Jamal, Adil
Hossain, Md. Belal
Ahmad, Zulfiqar
Mansoor, Muhammad Khalid
Khan, Iahtasham
Zahra, Kainat
Khan, Ahrar
Histopathological Investigations and Molecular Confirmation Reveal Mycobacterium bovis in One-Horned Rhinoceros (Rhinoceros unicorns)
title Histopathological Investigations and Molecular Confirmation Reveal Mycobacterium bovis in One-Horned Rhinoceros (Rhinoceros unicorns)
title_full Histopathological Investigations and Molecular Confirmation Reveal Mycobacterium bovis in One-Horned Rhinoceros (Rhinoceros unicorns)
title_fullStr Histopathological Investigations and Molecular Confirmation Reveal Mycobacterium bovis in One-Horned Rhinoceros (Rhinoceros unicorns)
title_full_unstemmed Histopathological Investigations and Molecular Confirmation Reveal Mycobacterium bovis in One-Horned Rhinoceros (Rhinoceros unicorns)
title_short Histopathological Investigations and Molecular Confirmation Reveal Mycobacterium bovis in One-Horned Rhinoceros (Rhinoceros unicorns)
title_sort histopathological investigations and molecular confirmation reveal mycobacterium bovis in one-horned rhinoceros (rhinoceros unicorns)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132650/
https://www.ncbi.nlm.nih.gov/pubmed/35647178
http://dx.doi.org/10.1155/2022/5816986
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