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Lake microbiome and trophy fluctuations of the ancient hemp rettery
Lake sediments not only store the long-term ecological information including pollen and microfossils but are also a source of sedimentary DNA (sedDNA). Here, by the combination of traditional multi-proxy paleolimnological methods with the whole-metagenome shotgun-sequencing of sedDNA we were able to...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132974/ https://www.ncbi.nlm.nih.gov/pubmed/35614182 http://dx.doi.org/10.1038/s41598-022-12761-w |
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author | Iwańska, Olga Latoch, Przemysław Suchora, Magdalena Pidek, Irena A. Huber, Miłosz Bubak, Iwona Kopik, Natalia Kovalenko, Mariia Gąsiorowski, Michał Armache, Jean-Paul Starosta, Agata L. |
author_facet | Iwańska, Olga Latoch, Przemysław Suchora, Magdalena Pidek, Irena A. Huber, Miłosz Bubak, Iwona Kopik, Natalia Kovalenko, Mariia Gąsiorowski, Michał Armache, Jean-Paul Starosta, Agata L. |
author_sort | Iwańska, Olga |
collection | PubMed |
description | Lake sediments not only store the long-term ecological information including pollen and microfossils but are also a source of sedimentary DNA (sedDNA). Here, by the combination of traditional multi-proxy paleolimnological methods with the whole-metagenome shotgun-sequencing of sedDNA we were able to paint a comprehensive picture of the fluctuations in trophy and bacterial diversity and metabolism of a small temperate lake in response to hemp retting, across the past 2000 years. Hemp retting (HR), a key step in hemp fibre production, was historically carried out in freshwater reservoirs and had a negative impact on the lake ecosystems. In Lake Slone, we identified two HR events, during the late stage of the Roman and Early Medieval periods and correlated these to the increased trophy and imbalanced lake microbiome. The metagenomic analyses showed a higher abundance of Chloroflexi, Planctomycetes and Bacteroidetes and a functional shift towards anaerobic metabolism, including degradation of complex biopolymers such as pectin and cellulose, during HR episodes. The lake eutrophication during HR was linked to the allochthonous, rather than autochthonous carbon supply—hemp straws. We also showed that the identification of HR based on the palynological analysis of hemp pollen may be inconclusive and we suggest the employment of the fibre count analysis as an additional and independent proxy. |
format | Online Article Text |
id | pubmed-9132974 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-91329742022-05-27 Lake microbiome and trophy fluctuations of the ancient hemp rettery Iwańska, Olga Latoch, Przemysław Suchora, Magdalena Pidek, Irena A. Huber, Miłosz Bubak, Iwona Kopik, Natalia Kovalenko, Mariia Gąsiorowski, Michał Armache, Jean-Paul Starosta, Agata L. Sci Rep Article Lake sediments not only store the long-term ecological information including pollen and microfossils but are also a source of sedimentary DNA (sedDNA). Here, by the combination of traditional multi-proxy paleolimnological methods with the whole-metagenome shotgun-sequencing of sedDNA we were able to paint a comprehensive picture of the fluctuations in trophy and bacterial diversity and metabolism of a small temperate lake in response to hemp retting, across the past 2000 years. Hemp retting (HR), a key step in hemp fibre production, was historically carried out in freshwater reservoirs and had a negative impact on the lake ecosystems. In Lake Slone, we identified two HR events, during the late stage of the Roman and Early Medieval periods and correlated these to the increased trophy and imbalanced lake microbiome. The metagenomic analyses showed a higher abundance of Chloroflexi, Planctomycetes and Bacteroidetes and a functional shift towards anaerobic metabolism, including degradation of complex biopolymers such as pectin and cellulose, during HR episodes. The lake eutrophication during HR was linked to the allochthonous, rather than autochthonous carbon supply—hemp straws. We also showed that the identification of HR based on the palynological analysis of hemp pollen may be inconclusive and we suggest the employment of the fibre count analysis as an additional and independent proxy. Nature Publishing Group UK 2022-05-25 /pmc/articles/PMC9132974/ /pubmed/35614182 http://dx.doi.org/10.1038/s41598-022-12761-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Iwańska, Olga Latoch, Przemysław Suchora, Magdalena Pidek, Irena A. Huber, Miłosz Bubak, Iwona Kopik, Natalia Kovalenko, Mariia Gąsiorowski, Michał Armache, Jean-Paul Starosta, Agata L. Lake microbiome and trophy fluctuations of the ancient hemp rettery |
title | Lake microbiome and trophy fluctuations of the ancient hemp rettery |
title_full | Lake microbiome and trophy fluctuations of the ancient hemp rettery |
title_fullStr | Lake microbiome and trophy fluctuations of the ancient hemp rettery |
title_full_unstemmed | Lake microbiome and trophy fluctuations of the ancient hemp rettery |
title_short | Lake microbiome and trophy fluctuations of the ancient hemp rettery |
title_sort | lake microbiome and trophy fluctuations of the ancient hemp rettery |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132974/ https://www.ncbi.nlm.nih.gov/pubmed/35614182 http://dx.doi.org/10.1038/s41598-022-12761-w |
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