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Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis
In the ongoing COVID-19 pandemic, rapid and sensitive diagnosis of viral infection is a critical deterrent to the spread of SARS-CoV-2. To this end, we developed an automated amplification-free digital RNA detection platform using CRISPR-Cas13a and microchamber device (opn-SATORI), which automatical...
| Autores principales: | , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132978/ https://www.ncbi.nlm.nih.gov/pubmed/35614128 http://dx.doi.org/10.1038/s42003-022-03433-6 |
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| author | Shinoda, Hajime Iida, Tatsuya Makino, Asami Yoshimura, Mami Ishikawa, Junichiro Ando, Jun Murai, Kazue Sugiyama, Katsumi Muramoto, Yukiko Nakano, Masahiro Kiga, Kotaro Cui, Longzhu Nureki, Osamu Takeuchi, Hiroaki Noda, Takeshi Nishimasu, Hiroshi Watanabe, Rikiya |
| author_facet | Shinoda, Hajime Iida, Tatsuya Makino, Asami Yoshimura, Mami Ishikawa, Junichiro Ando, Jun Murai, Kazue Sugiyama, Katsumi Muramoto, Yukiko Nakano, Masahiro Kiga, Kotaro Cui, Longzhu Nureki, Osamu Takeuchi, Hiroaki Noda, Takeshi Nishimasu, Hiroshi Watanabe, Rikiya |
| author_sort | Shinoda, Hajime |
| collection | PubMed |
| description | In the ongoing COVID-19 pandemic, rapid and sensitive diagnosis of viral infection is a critical deterrent to the spread of SARS-CoV-2. To this end, we developed an automated amplification-free digital RNA detection platform using CRISPR-Cas13a and microchamber device (opn-SATORI), which automatically completes a detection process from sample mixing to RNA quantification in clinical specimens within ~9 min. Using the optimal Cas13a enzyme and magnetic beads technology, opn-SATORI detected SARS-CoV-2 genomic RNA with a LoD of < 6.5 aM (3.9 copies μL(−1)), comparable to RT-qPCR. Additionally, opn-SATORI discriminated between SARS-CoV-2 variants of concern, including alpha, delta, and omicron, with 98% accuracy. Thus, opn-SATORI can serve as a rapid and convenient diagnostic platform for identifying several types of viral infections. |
| format | Online Article Text |
| id | pubmed-9132978 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-91329782022-05-27 Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis Shinoda, Hajime Iida, Tatsuya Makino, Asami Yoshimura, Mami Ishikawa, Junichiro Ando, Jun Murai, Kazue Sugiyama, Katsumi Muramoto, Yukiko Nakano, Masahiro Kiga, Kotaro Cui, Longzhu Nureki, Osamu Takeuchi, Hiroaki Noda, Takeshi Nishimasu, Hiroshi Watanabe, Rikiya Commun Biol Article In the ongoing COVID-19 pandemic, rapid and sensitive diagnosis of viral infection is a critical deterrent to the spread of SARS-CoV-2. To this end, we developed an automated amplification-free digital RNA detection platform using CRISPR-Cas13a and microchamber device (opn-SATORI), which automatically completes a detection process from sample mixing to RNA quantification in clinical specimens within ~9 min. Using the optimal Cas13a enzyme and magnetic beads technology, opn-SATORI detected SARS-CoV-2 genomic RNA with a LoD of < 6.5 aM (3.9 copies μL(−1)), comparable to RT-qPCR. Additionally, opn-SATORI discriminated between SARS-CoV-2 variants of concern, including alpha, delta, and omicron, with 98% accuracy. Thus, opn-SATORI can serve as a rapid and convenient diagnostic platform for identifying several types of viral infections. Nature Publishing Group UK 2022-05-26 /pmc/articles/PMC9132978/ /pubmed/35614128 http://dx.doi.org/10.1038/s42003-022-03433-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
| spellingShingle | Article Shinoda, Hajime Iida, Tatsuya Makino, Asami Yoshimura, Mami Ishikawa, Junichiro Ando, Jun Murai, Kazue Sugiyama, Katsumi Muramoto, Yukiko Nakano, Masahiro Kiga, Kotaro Cui, Longzhu Nureki, Osamu Takeuchi, Hiroaki Noda, Takeshi Nishimasu, Hiroshi Watanabe, Rikiya Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis |
| title | Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis |
| title_full | Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis |
| title_fullStr | Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis |
| title_full_unstemmed | Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis |
| title_short | Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis |
| title_sort | automated amplification-free digital rna detection platform for rapid and sensitive sars-cov-2 diagnosis |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132978/ https://www.ncbi.nlm.nih.gov/pubmed/35614128 http://dx.doi.org/10.1038/s42003-022-03433-6 |
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