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Selection of suitable candidate genes for mRNA expression normalization in bulbil development of Pinellia ternata

Pinellia ternata (Thunb.) Breit. (Abbreviated as P. ternata). It is a commonly prescribed Chinese traditional medicinal herb for the treatment of phlegm, cough, and morning sick. Bulbil reproduction is one of the main reproductive methods of P. ternata. The accurate quantification of gene expression...

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Autores principales: Fan, Haoyu, He, Qiuling, Dong, Yiheng, Xu, Wenxin, Lou, Yanlin, Hua, Xuejun, Xu, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133075/
https://www.ncbi.nlm.nih.gov/pubmed/35614175
http://dx.doi.org/10.1038/s41598-022-12782-5
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author Fan, Haoyu
He, Qiuling
Dong, Yiheng
Xu, Wenxin
Lou, Yanlin
Hua, Xuejun
Xu, Tao
author_facet Fan, Haoyu
He, Qiuling
Dong, Yiheng
Xu, Wenxin
Lou, Yanlin
Hua, Xuejun
Xu, Tao
author_sort Fan, Haoyu
collection PubMed
description Pinellia ternata (Thunb.) Breit. (Abbreviated as P. ternata). It is a commonly prescribed Chinese traditional medicinal herb for the treatment of phlegm, cough, and morning sick. Bulbil reproduction is one of the main reproductive methods of P. ternata. The accurate quantification of gene expression patterns associated with bulbil development might be helpful to explore the molecular mechanism involved in P. ternata reproduction. Quantitative real-time PCR was the most preferred method for expression profile and function analysis of mRNA. However, the reference genes in different tissues of P. ternata in different periods of bulbil development have not been studied in detail. In present study, the expression stability of eight candidate reference genes were determined with programs: geNorm, NormFinder, BestKeeper, and refFinder. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified as the top- rated reference gene in all samples of P. ternata, while different combinations of reference gene proved to be the most stable depending on development stage and tissue type. Furthermore, the reliability of GAPDH expression was verified by six P. ternata related genes in hormone and nutrient biosynthesis pathways, and the expression profiles of these genes were agreed with the results of RNA-seq digital gene expression analysis. These results can contribute to studies of gene expression patterns and functional analysis of P. ternata involved in bulbil development.
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spelling pubmed-91330752022-05-27 Selection of suitable candidate genes for mRNA expression normalization in bulbil development of Pinellia ternata Fan, Haoyu He, Qiuling Dong, Yiheng Xu, Wenxin Lou, Yanlin Hua, Xuejun Xu, Tao Sci Rep Article Pinellia ternata (Thunb.) Breit. (Abbreviated as P. ternata). It is a commonly prescribed Chinese traditional medicinal herb for the treatment of phlegm, cough, and morning sick. Bulbil reproduction is one of the main reproductive methods of P. ternata. The accurate quantification of gene expression patterns associated with bulbil development might be helpful to explore the molecular mechanism involved in P. ternata reproduction. Quantitative real-time PCR was the most preferred method for expression profile and function analysis of mRNA. However, the reference genes in different tissues of P. ternata in different periods of bulbil development have not been studied in detail. In present study, the expression stability of eight candidate reference genes were determined with programs: geNorm, NormFinder, BestKeeper, and refFinder. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified as the top- rated reference gene in all samples of P. ternata, while different combinations of reference gene proved to be the most stable depending on development stage and tissue type. Furthermore, the reliability of GAPDH expression was verified by six P. ternata related genes in hormone and nutrient biosynthesis pathways, and the expression profiles of these genes were agreed with the results of RNA-seq digital gene expression analysis. These results can contribute to studies of gene expression patterns and functional analysis of P. ternata involved in bulbil development. Nature Publishing Group UK 2022-05-25 /pmc/articles/PMC9133075/ /pubmed/35614175 http://dx.doi.org/10.1038/s41598-022-12782-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Fan, Haoyu
He, Qiuling
Dong, Yiheng
Xu, Wenxin
Lou, Yanlin
Hua, Xuejun
Xu, Tao
Selection of suitable candidate genes for mRNA expression normalization in bulbil development of Pinellia ternata
title Selection of suitable candidate genes for mRNA expression normalization in bulbil development of Pinellia ternata
title_full Selection of suitable candidate genes for mRNA expression normalization in bulbil development of Pinellia ternata
title_fullStr Selection of suitable candidate genes for mRNA expression normalization in bulbil development of Pinellia ternata
title_full_unstemmed Selection of suitable candidate genes for mRNA expression normalization in bulbil development of Pinellia ternata
title_short Selection of suitable candidate genes for mRNA expression normalization in bulbil development of Pinellia ternata
title_sort selection of suitable candidate genes for mrna expression normalization in bulbil development of pinellia ternata
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133075/
https://www.ncbi.nlm.nih.gov/pubmed/35614175
http://dx.doi.org/10.1038/s41598-022-12782-5
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