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Forward Genetics in Apicomplexa Biology: The Host Side of the Story
Forward genetic approaches have been widely used in parasitology and have proven their power to reveal the complexities of host-parasite interactions in an unbiased fashion. Many aspects of the parasite’s biology, including the identification of virulence factors, replication determinants, antibioti...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133346/ https://www.ncbi.nlm.nih.gov/pubmed/35646724 http://dx.doi.org/10.3389/fcimb.2022.878475 |
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author | Sánchez-Arcila, Juan C. Jensen, Kirk D. C. |
author_facet | Sánchez-Arcila, Juan C. Jensen, Kirk D. C. |
author_sort | Sánchez-Arcila, Juan C. |
collection | PubMed |
description | Forward genetic approaches have been widely used in parasitology and have proven their power to reveal the complexities of host-parasite interactions in an unbiased fashion. Many aspects of the parasite’s biology, including the identification of virulence factors, replication determinants, antibiotic resistance genes, and other factors required for parasitic life, have been discovered using such strategies. Forward genetic approaches have also been employed to understand host resistance mechanisms to parasitic infection. Here, we will introduce and review all forward genetic approaches that have been used to identify host factors involved with Apicomplexa infections, which include classical genetic screens and QTL mapping, GWAS, ENU mutagenesis, overexpression, RNAi and CRISPR-Cas9 library screens. Collectively, these screens have improved our understanding of host resistance mechanisms, immune regulation, vaccine and drug designs for Apicomplexa parasites. We will also discuss how recent advances in molecular genetics give present opportunities to further explore host-parasite relationships. |
format | Online Article Text |
id | pubmed-9133346 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91333462022-05-27 Forward Genetics in Apicomplexa Biology: The Host Side of the Story Sánchez-Arcila, Juan C. Jensen, Kirk D. C. Front Cell Infect Microbiol Cellular and Infection Microbiology Forward genetic approaches have been widely used in parasitology and have proven their power to reveal the complexities of host-parasite interactions in an unbiased fashion. Many aspects of the parasite’s biology, including the identification of virulence factors, replication determinants, antibiotic resistance genes, and other factors required for parasitic life, have been discovered using such strategies. Forward genetic approaches have also been employed to understand host resistance mechanisms to parasitic infection. Here, we will introduce and review all forward genetic approaches that have been used to identify host factors involved with Apicomplexa infections, which include classical genetic screens and QTL mapping, GWAS, ENU mutagenesis, overexpression, RNAi and CRISPR-Cas9 library screens. Collectively, these screens have improved our understanding of host resistance mechanisms, immune regulation, vaccine and drug designs for Apicomplexa parasites. We will also discuss how recent advances in molecular genetics give present opportunities to further explore host-parasite relationships. Frontiers Media S.A. 2022-05-12 /pmc/articles/PMC9133346/ /pubmed/35646724 http://dx.doi.org/10.3389/fcimb.2022.878475 Text en Copyright © 2022 Sánchez-Arcila and Jensen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Sánchez-Arcila, Juan C. Jensen, Kirk D. C. Forward Genetics in Apicomplexa Biology: The Host Side of the Story |
title | Forward Genetics in Apicomplexa Biology: The Host Side of the Story |
title_full | Forward Genetics in Apicomplexa Biology: The Host Side of the Story |
title_fullStr | Forward Genetics in Apicomplexa Biology: The Host Side of the Story |
title_full_unstemmed | Forward Genetics in Apicomplexa Biology: The Host Side of the Story |
title_short | Forward Genetics in Apicomplexa Biology: The Host Side of the Story |
title_sort | forward genetics in apicomplexa biology: the host side of the story |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133346/ https://www.ncbi.nlm.nih.gov/pubmed/35646724 http://dx.doi.org/10.3389/fcimb.2022.878475 |
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