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Ex Vivo Irradiation of Lung Cancer Stem Cells Identifies the Lowest Therapeutic Dose Needed for Tumor Growth Arrest and Mass Reduction In Vivo

Radiotherapy represents a first-line treatment for many inoperable lung tumors. New technologies offer novel opportunities for the treatment of lung cancer with the administration of higher doses of radiation in smaller volumes. Because both therapeutic and toxic treatment effects are dose-dependent...

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Autores principales: Puglisi, Caterina, Giuffrida, Raffaella, Borzì, Giuseppina, Illari, Salvatore, Caronia, Francesco Paolo, Di Mattia, Paolo, Colarossi, Cristina, Ferini, Gianluca, Martorana, Emanuele, Sette, Giovanni, Eramo, Adriana, Lorico, Aurelio, Di Grazia, Alfio, Forte, Stefano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133629/
https://www.ncbi.nlm.nih.gov/pubmed/35646627
http://dx.doi.org/10.3389/fonc.2022.837400
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author Puglisi, Caterina
Giuffrida, Raffaella
Borzì, Giuseppina
Illari, Salvatore
Caronia, Francesco Paolo
Di Mattia, Paolo
Colarossi, Cristina
Ferini, Gianluca
Martorana, Emanuele
Sette, Giovanni
Eramo, Adriana
Lorico, Aurelio
Di Grazia, Alfio
Forte, Stefano
author_facet Puglisi, Caterina
Giuffrida, Raffaella
Borzì, Giuseppina
Illari, Salvatore
Caronia, Francesco Paolo
Di Mattia, Paolo
Colarossi, Cristina
Ferini, Gianluca
Martorana, Emanuele
Sette, Giovanni
Eramo, Adriana
Lorico, Aurelio
Di Grazia, Alfio
Forte, Stefano
author_sort Puglisi, Caterina
collection PubMed
description Radiotherapy represents a first-line treatment for many inoperable lung tumors. New technologies offer novel opportunities for the treatment of lung cancer with the administration of higher doses of radiation in smaller volumes. Because both therapeutic and toxic treatment effects are dose-dependent, it is important to identify a minimal dose protocol for each individual patient that maintains efficacy while decreasing toxicity. Cancer stem cells sustain tumor growth, promote metastatic dissemination, and may give rise to secondary resistance. The identification of effective protocols targeting these cells may improve disease-free survival of treated patients. In this work, we evaluated the existence of individual profiles of sensitivity to radiotherapy in patient-derived cancer stem cells (CSCs) using both in vitro and in vivo models. Both CSCs in vitro and mice implanted with CSCs were treated with radiotherapy at different dose intensities and rates. CSC response to different radiation doses greatly varied among patients. In vitro radiation sensitivity of CSCs corresponded to the therapeutic outcome in the corresponding mouse tumor model. On the other side, the dose administration rate did not affect the response. These findings suggest that in vitro evaluation of CSCs may potentially predict patients’ response, thus guiding clinical decision.
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spelling pubmed-91336292022-05-27 Ex Vivo Irradiation of Lung Cancer Stem Cells Identifies the Lowest Therapeutic Dose Needed for Tumor Growth Arrest and Mass Reduction In Vivo Puglisi, Caterina Giuffrida, Raffaella Borzì, Giuseppina Illari, Salvatore Caronia, Francesco Paolo Di Mattia, Paolo Colarossi, Cristina Ferini, Gianluca Martorana, Emanuele Sette, Giovanni Eramo, Adriana Lorico, Aurelio Di Grazia, Alfio Forte, Stefano Front Oncol Oncology Radiotherapy represents a first-line treatment for many inoperable lung tumors. New technologies offer novel opportunities for the treatment of lung cancer with the administration of higher doses of radiation in smaller volumes. Because both therapeutic and toxic treatment effects are dose-dependent, it is important to identify a minimal dose protocol for each individual patient that maintains efficacy while decreasing toxicity. Cancer stem cells sustain tumor growth, promote metastatic dissemination, and may give rise to secondary resistance. The identification of effective protocols targeting these cells may improve disease-free survival of treated patients. In this work, we evaluated the existence of individual profiles of sensitivity to radiotherapy in patient-derived cancer stem cells (CSCs) using both in vitro and in vivo models. Both CSCs in vitro and mice implanted with CSCs were treated with radiotherapy at different dose intensities and rates. CSC response to different radiation doses greatly varied among patients. In vitro radiation sensitivity of CSCs corresponded to the therapeutic outcome in the corresponding mouse tumor model. On the other side, the dose administration rate did not affect the response. These findings suggest that in vitro evaluation of CSCs may potentially predict patients’ response, thus guiding clinical decision. Frontiers Media S.A. 2022-05-12 /pmc/articles/PMC9133629/ /pubmed/35646627 http://dx.doi.org/10.3389/fonc.2022.837400 Text en Copyright © 2022 Puglisi, Giuffrida, Borzì, Illari, Caronia, Di Mattia, Colarossi, Ferini, Martorana, Sette, Eramo, Lorico, Di Grazia and Forte https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Puglisi, Caterina
Giuffrida, Raffaella
Borzì, Giuseppina
Illari, Salvatore
Caronia, Francesco Paolo
Di Mattia, Paolo
Colarossi, Cristina
Ferini, Gianluca
Martorana, Emanuele
Sette, Giovanni
Eramo, Adriana
Lorico, Aurelio
Di Grazia, Alfio
Forte, Stefano
Ex Vivo Irradiation of Lung Cancer Stem Cells Identifies the Lowest Therapeutic Dose Needed for Tumor Growth Arrest and Mass Reduction In Vivo
title Ex Vivo Irradiation of Lung Cancer Stem Cells Identifies the Lowest Therapeutic Dose Needed for Tumor Growth Arrest and Mass Reduction In Vivo
title_full Ex Vivo Irradiation of Lung Cancer Stem Cells Identifies the Lowest Therapeutic Dose Needed for Tumor Growth Arrest and Mass Reduction In Vivo
title_fullStr Ex Vivo Irradiation of Lung Cancer Stem Cells Identifies the Lowest Therapeutic Dose Needed for Tumor Growth Arrest and Mass Reduction In Vivo
title_full_unstemmed Ex Vivo Irradiation of Lung Cancer Stem Cells Identifies the Lowest Therapeutic Dose Needed for Tumor Growth Arrest and Mass Reduction In Vivo
title_short Ex Vivo Irradiation of Lung Cancer Stem Cells Identifies the Lowest Therapeutic Dose Needed for Tumor Growth Arrest and Mass Reduction In Vivo
title_sort ex vivo irradiation of lung cancer stem cells identifies the lowest therapeutic dose needed for tumor growth arrest and mass reduction in vivo
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133629/
https://www.ncbi.nlm.nih.gov/pubmed/35646627
http://dx.doi.org/10.3389/fonc.2022.837400
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