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Engineered three-dimensional cardiac tissues maturing in a rotating wall vessel bioreactor remodel diseased hearts in rats with myocardial infarction

A rotating wall vessel (RWV) bioreactor was constructed for growing massive functional cardiac constructs to recover the function of a distressed rat heart. Three-dimensional cardiac tissues were engineered by seeding human-induced pluripotent stem cell-derived cardiomyocytes on poly(lactic-co-glyco...

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Autores principales: Nakazato, Taro, Kawamura, Takuji, Uemura, Toshimasa, Liu, Li, Li, Junjun, Sasai, Masao, Harada, Akima, Ito, Emiko, Iseoka, Hiroko, Toda, Koichi, Sawa, Yoshiki, Miyagawa, Shigeru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133656/
https://www.ncbi.nlm.nih.gov/pubmed/35427484
http://dx.doi.org/10.1016/j.stemcr.2022.03.012
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author Nakazato, Taro
Kawamura, Takuji
Uemura, Toshimasa
Liu, Li
Li, Junjun
Sasai, Masao
Harada, Akima
Ito, Emiko
Iseoka, Hiroko
Toda, Koichi
Sawa, Yoshiki
Miyagawa, Shigeru
author_facet Nakazato, Taro
Kawamura, Takuji
Uemura, Toshimasa
Liu, Li
Li, Junjun
Sasai, Masao
Harada, Akima
Ito, Emiko
Iseoka, Hiroko
Toda, Koichi
Sawa, Yoshiki
Miyagawa, Shigeru
author_sort Nakazato, Taro
collection PubMed
description A rotating wall vessel (RWV) bioreactor was constructed for growing massive functional cardiac constructs to recover the function of a distressed rat heart. Three-dimensional cardiac tissues were engineered by seeding human-induced pluripotent stem cell-derived cardiomyocytes on poly(lactic-co-glycolic acid) fiber sheets (3D-hiPSC-CTs) and cultured in the RWV bioreactor (RWV group) or under static conditions (control group). The tissues were transplanted into a myocardial infarction nude rat model, and cardiac performance was evaluated. In the RWV group, cell viability and contractile and electrical properties significantly improved, mature cardiomyocytes were observed, and mechanical stress-related mediators of mammalian target of rapamycin signaling were upregulated compared with those of the control. Four weeks post-transplantation, tissue survival and left ventricular ejection fraction significantly improved in the RWV group. Hence, dynamic culture in an RWV bioreactor could provide a superior culture environment for improved performance of 3D-hiPSC-CTs, providing a means for functional cardiomyogenesis in myocyte-loss heart failure.
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spelling pubmed-91336562022-05-27 Engineered three-dimensional cardiac tissues maturing in a rotating wall vessel bioreactor remodel diseased hearts in rats with myocardial infarction Nakazato, Taro Kawamura, Takuji Uemura, Toshimasa Liu, Li Li, Junjun Sasai, Masao Harada, Akima Ito, Emiko Iseoka, Hiroko Toda, Koichi Sawa, Yoshiki Miyagawa, Shigeru Stem Cell Reports Article A rotating wall vessel (RWV) bioreactor was constructed for growing massive functional cardiac constructs to recover the function of a distressed rat heart. Three-dimensional cardiac tissues were engineered by seeding human-induced pluripotent stem cell-derived cardiomyocytes on poly(lactic-co-glycolic acid) fiber sheets (3D-hiPSC-CTs) and cultured in the RWV bioreactor (RWV group) or under static conditions (control group). The tissues were transplanted into a myocardial infarction nude rat model, and cardiac performance was evaluated. In the RWV group, cell viability and contractile and electrical properties significantly improved, mature cardiomyocytes were observed, and mechanical stress-related mediators of mammalian target of rapamycin signaling were upregulated compared with those of the control. Four weeks post-transplantation, tissue survival and left ventricular ejection fraction significantly improved in the RWV group. Hence, dynamic culture in an RWV bioreactor could provide a superior culture environment for improved performance of 3D-hiPSC-CTs, providing a means for functional cardiomyogenesis in myocyte-loss heart failure. Elsevier 2022-04-14 /pmc/articles/PMC9133656/ /pubmed/35427484 http://dx.doi.org/10.1016/j.stemcr.2022.03.012 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Nakazato, Taro
Kawamura, Takuji
Uemura, Toshimasa
Liu, Li
Li, Junjun
Sasai, Masao
Harada, Akima
Ito, Emiko
Iseoka, Hiroko
Toda, Koichi
Sawa, Yoshiki
Miyagawa, Shigeru
Engineered three-dimensional cardiac tissues maturing in a rotating wall vessel bioreactor remodel diseased hearts in rats with myocardial infarction
title Engineered three-dimensional cardiac tissues maturing in a rotating wall vessel bioreactor remodel diseased hearts in rats with myocardial infarction
title_full Engineered three-dimensional cardiac tissues maturing in a rotating wall vessel bioreactor remodel diseased hearts in rats with myocardial infarction
title_fullStr Engineered three-dimensional cardiac tissues maturing in a rotating wall vessel bioreactor remodel diseased hearts in rats with myocardial infarction
title_full_unstemmed Engineered three-dimensional cardiac tissues maturing in a rotating wall vessel bioreactor remodel diseased hearts in rats with myocardial infarction
title_short Engineered three-dimensional cardiac tissues maturing in a rotating wall vessel bioreactor remodel diseased hearts in rats with myocardial infarction
title_sort engineered three-dimensional cardiac tissues maturing in a rotating wall vessel bioreactor remodel diseased hearts in rats with myocardial infarction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133656/
https://www.ncbi.nlm.nih.gov/pubmed/35427484
http://dx.doi.org/10.1016/j.stemcr.2022.03.012
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