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Molecular Detection of Parvovirus in Captive Siberian Tigers and Lions in Northeastern China From 2019 to 2021

The fact that wild felines are carriers of pernicious infectious viruses should be a major concern due to the potential cross-species transmission between the felines and human or domestic animals. However, studies on the virus in the captive wild felines, especially in tigers, are thin on the groun...

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Autores principales: Huang, Shuping, Li, Xiang, Xie, Wei, Guo, Lijun, You, Dan, Xu, Haitao, Liu, Dan, Wang, Yulong, Hou, Zhijun, Zeng, Xiangwei, Yang, Siyuan, Chai, Hongliang, Wang, Yajun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133805/
https://www.ncbi.nlm.nih.gov/pubmed/35633695
http://dx.doi.org/10.3389/fmicb.2022.898184
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author Huang, Shuping
Li, Xiang
Xie, Wei
Guo, Lijun
You, Dan
Xu, Haitao
Liu, Dan
Wang, Yulong
Hou, Zhijun
Zeng, Xiangwei
Yang, Siyuan
Chai, Hongliang
Wang, Yajun
author_facet Huang, Shuping
Li, Xiang
Xie, Wei
Guo, Lijun
You, Dan
Xu, Haitao
Liu, Dan
Wang, Yulong
Hou, Zhijun
Zeng, Xiangwei
Yang, Siyuan
Chai, Hongliang
Wang, Yajun
author_sort Huang, Shuping
collection PubMed
description The fact that wild felines are carriers of pernicious infectious viruses should be a major concern due to the potential cross-species transmission between the felines and human or domestic animals. However, studies on the virus in the captive wild felines, especially in tigers, are thin on the ground. In this study, we screened four infectious viruses, namely, feline parvovirus (FPV), feline coronavirus (FCoV), canine distemper virus (CDV), and influenza A virus (IAV), in the blood samples of 285 captive Siberian tigers (Panthera tigris altaica) and in the spleen samples of two deceased lions (Panthera leo), which were collected from 2019 to 2021 in three Siberian Tiger Parks from the northeast of China. Nucleic acids isolated from the blood samples collected from tigers and the spleen samples collected from two deceased lions were positive for FPV by PCR, and the positive rate was 4.6% (13/285) in tigers. Furthermore, the VP2 gene of FPV was amplified by nested PCR, and the sequences of the VP2 gene from these six FPV positive strains shared 98.3–99.9% homology with the reference. The key amino acid sites of VP2 protein were consistent with that of FPV reference strains. Phylogenetic analysis based on the VP2 gene showed that in this study, FPV-positive strains were grouped within the FPV clade and closely related to the Asian strains clade. The results of this study showed that FPV circulated in the captive Siberian tigers and lions in northeastern China and provided valuable information for the study of FPV epidemiology in wild felines. Therefore, we suggest that regular antibody monitoring and booster immunization for tigers should be performed.
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spelling pubmed-91338052022-05-27 Molecular Detection of Parvovirus in Captive Siberian Tigers and Lions in Northeastern China From 2019 to 2021 Huang, Shuping Li, Xiang Xie, Wei Guo, Lijun You, Dan Xu, Haitao Liu, Dan Wang, Yulong Hou, Zhijun Zeng, Xiangwei Yang, Siyuan Chai, Hongliang Wang, Yajun Front Microbiol Microbiology The fact that wild felines are carriers of pernicious infectious viruses should be a major concern due to the potential cross-species transmission between the felines and human or domestic animals. However, studies on the virus in the captive wild felines, especially in tigers, are thin on the ground. In this study, we screened four infectious viruses, namely, feline parvovirus (FPV), feline coronavirus (FCoV), canine distemper virus (CDV), and influenza A virus (IAV), in the blood samples of 285 captive Siberian tigers (Panthera tigris altaica) and in the spleen samples of two deceased lions (Panthera leo), which were collected from 2019 to 2021 in three Siberian Tiger Parks from the northeast of China. Nucleic acids isolated from the blood samples collected from tigers and the spleen samples collected from two deceased lions were positive for FPV by PCR, and the positive rate was 4.6% (13/285) in tigers. Furthermore, the VP2 gene of FPV was amplified by nested PCR, and the sequences of the VP2 gene from these six FPV positive strains shared 98.3–99.9% homology with the reference. The key amino acid sites of VP2 protein were consistent with that of FPV reference strains. Phylogenetic analysis based on the VP2 gene showed that in this study, FPV-positive strains were grouped within the FPV clade and closely related to the Asian strains clade. The results of this study showed that FPV circulated in the captive Siberian tigers and lions in northeastern China and provided valuable information for the study of FPV epidemiology in wild felines. Therefore, we suggest that regular antibody monitoring and booster immunization for tigers should be performed. Frontiers Media S.A. 2022-05-12 /pmc/articles/PMC9133805/ /pubmed/35633695 http://dx.doi.org/10.3389/fmicb.2022.898184 Text en Copyright © 2022 Huang, Li, Xie, Guo, You, Xu, Liu, Wang, Hou, Zeng, Yang, Chai and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Huang, Shuping
Li, Xiang
Xie, Wei
Guo, Lijun
You, Dan
Xu, Haitao
Liu, Dan
Wang, Yulong
Hou, Zhijun
Zeng, Xiangwei
Yang, Siyuan
Chai, Hongliang
Wang, Yajun
Molecular Detection of Parvovirus in Captive Siberian Tigers and Lions in Northeastern China From 2019 to 2021
title Molecular Detection of Parvovirus in Captive Siberian Tigers and Lions in Northeastern China From 2019 to 2021
title_full Molecular Detection of Parvovirus in Captive Siberian Tigers and Lions in Northeastern China From 2019 to 2021
title_fullStr Molecular Detection of Parvovirus in Captive Siberian Tigers and Lions in Northeastern China From 2019 to 2021
title_full_unstemmed Molecular Detection of Parvovirus in Captive Siberian Tigers and Lions in Northeastern China From 2019 to 2021
title_short Molecular Detection of Parvovirus in Captive Siberian Tigers and Lions in Northeastern China From 2019 to 2021
title_sort molecular detection of parvovirus in captive siberian tigers and lions in northeastern china from 2019 to 2021
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133805/
https://www.ncbi.nlm.nih.gov/pubmed/35633695
http://dx.doi.org/10.3389/fmicb.2022.898184
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