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Performance of Single-Shot Echo-Planar Imaging in Diffusion Tensor Imaging in Rat Sciatic Nerve Compared With Readout-Segmented Echo-Planar Imaging

OBJECTIVES: To compare the performances of single-shot echo-planar imaging (SS–EPI) and readout-segmented echo-planar imaging (RS–EPI) for diffusion tensor imaging (DTI) of the rat sciatic nerve. METHODS: Eight healthy adult male Sprague-Dawley rats were anesthetized and scanned with a 3T MRI scanne...

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Autores principales: Chen, Yueyao, Pan, Zhongxian, Meng, Fanqi, Li, Zhujing, Hu, Yuanming, Yu, Xuewen, Gao, Jinyun, Guo, Yihao, Lyu, Hanqing, Lin, Xiaofeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133884/
https://www.ncbi.nlm.nih.gov/pubmed/35645709
http://dx.doi.org/10.3389/fnins.2022.844408
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author Chen, Yueyao
Pan, Zhongxian
Meng, Fanqi
Li, Zhujing
Hu, Yuanming
Yu, Xuewen
Gao, Jinyun
Guo, Yihao
Lyu, Hanqing
Lin, Xiaofeng
author_facet Chen, Yueyao
Pan, Zhongxian
Meng, Fanqi
Li, Zhujing
Hu, Yuanming
Yu, Xuewen
Gao, Jinyun
Guo, Yihao
Lyu, Hanqing
Lin, Xiaofeng
author_sort Chen, Yueyao
collection PubMed
description OBJECTIVES: To compare the performances of single-shot echo-planar imaging (SS–EPI) and readout-segmented echo-planar imaging (RS–EPI) for diffusion tensor imaging (DTI) of the rat sciatic nerve. METHODS: Eight healthy adult male Sprague-Dawley rats were anesthetized and scanned with a 3T MRI scanner using SS–EPI and RS–EPI DTI sequences. The image quality in terms of the morphology of the nerve, distortions of the nearby femur, muscles, and homogeneity of neuromuscular were evaluated and scored. The correlations between the DTI parameters including fractional anisotropy (FA), axial diffusivity (AD), radial diffusivity (RD), apparent diffusion coefficient (ADC), and histopathological parameters were calculated by using the Pearson correlation coefficient and compared by the modified Fisher Z-transform, respectively. RESULTS: The quality scores were higher for the images from the SS–EPI sequence compared with the RS–EPI sequence for characteristics such as sharpness of the sciatic nerve margin (P = 0.008), artifacts of the sciatic nerve (P = 0.008), and homogeneity of the neuromuscular region (P = 0.007), as well as the contrast-to-noise ratio (CNR) of DW images (P < 0.001). The correlation coefficients were higher for the FA and RD values from the SS–EPI sequence compared with those from the RS–EPI sequence. Furthermore, the correlation coefficients between FA and myelin thickness (P = 0.027), FA and diameter of the myelinated fiber (P = 0.036), as well as RD and myelin thickness (P = 0.05) were statistically higher for the SS–EPI sequence compared with those for the RS–EPI sequence. CONCLUSION: Diffusion tensor imaging analysis of the rat sciatic nerve showed that the image quality from the SS–EPI sequence was significantly higher compared with that from the RS–EPI sequence. Furthermore, the FA and RD derived from the SS–EPI sequence are promising and sensitive biomarkers to detect the histopathological changes in the rat sciatic nerve.
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spelling pubmed-91338842022-05-27 Performance of Single-Shot Echo-Planar Imaging in Diffusion Tensor Imaging in Rat Sciatic Nerve Compared With Readout-Segmented Echo-Planar Imaging Chen, Yueyao Pan, Zhongxian Meng, Fanqi Li, Zhujing Hu, Yuanming Yu, Xuewen Gao, Jinyun Guo, Yihao Lyu, Hanqing Lin, Xiaofeng Front Neurosci Neuroscience OBJECTIVES: To compare the performances of single-shot echo-planar imaging (SS–EPI) and readout-segmented echo-planar imaging (RS–EPI) for diffusion tensor imaging (DTI) of the rat sciatic nerve. METHODS: Eight healthy adult male Sprague-Dawley rats were anesthetized and scanned with a 3T MRI scanner using SS–EPI and RS–EPI DTI sequences. The image quality in terms of the morphology of the nerve, distortions of the nearby femur, muscles, and homogeneity of neuromuscular were evaluated and scored. The correlations between the DTI parameters including fractional anisotropy (FA), axial diffusivity (AD), radial diffusivity (RD), apparent diffusion coefficient (ADC), and histopathological parameters were calculated by using the Pearson correlation coefficient and compared by the modified Fisher Z-transform, respectively. RESULTS: The quality scores were higher for the images from the SS–EPI sequence compared with the RS–EPI sequence for characteristics such as sharpness of the sciatic nerve margin (P = 0.008), artifacts of the sciatic nerve (P = 0.008), and homogeneity of the neuromuscular region (P = 0.007), as well as the contrast-to-noise ratio (CNR) of DW images (P < 0.001). The correlation coefficients were higher for the FA and RD values from the SS–EPI sequence compared with those from the RS–EPI sequence. Furthermore, the correlation coefficients between FA and myelin thickness (P = 0.027), FA and diameter of the myelinated fiber (P = 0.036), as well as RD and myelin thickness (P = 0.05) were statistically higher for the SS–EPI sequence compared with those for the RS–EPI sequence. CONCLUSION: Diffusion tensor imaging analysis of the rat sciatic nerve showed that the image quality from the SS–EPI sequence was significantly higher compared with that from the RS–EPI sequence. Furthermore, the FA and RD derived from the SS–EPI sequence are promising and sensitive biomarkers to detect the histopathological changes in the rat sciatic nerve. Frontiers Media S.A. 2022-05-12 /pmc/articles/PMC9133884/ /pubmed/35645709 http://dx.doi.org/10.3389/fnins.2022.844408 Text en Copyright © 2022 Chen, Pan, Meng, Li, Hu, Yu, Gao, Guo, Lyu and Lin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Chen, Yueyao
Pan, Zhongxian
Meng, Fanqi
Li, Zhujing
Hu, Yuanming
Yu, Xuewen
Gao, Jinyun
Guo, Yihao
Lyu, Hanqing
Lin, Xiaofeng
Performance of Single-Shot Echo-Planar Imaging in Diffusion Tensor Imaging in Rat Sciatic Nerve Compared With Readout-Segmented Echo-Planar Imaging
title Performance of Single-Shot Echo-Planar Imaging in Diffusion Tensor Imaging in Rat Sciatic Nerve Compared With Readout-Segmented Echo-Planar Imaging
title_full Performance of Single-Shot Echo-Planar Imaging in Diffusion Tensor Imaging in Rat Sciatic Nerve Compared With Readout-Segmented Echo-Planar Imaging
title_fullStr Performance of Single-Shot Echo-Planar Imaging in Diffusion Tensor Imaging in Rat Sciatic Nerve Compared With Readout-Segmented Echo-Planar Imaging
title_full_unstemmed Performance of Single-Shot Echo-Planar Imaging in Diffusion Tensor Imaging in Rat Sciatic Nerve Compared With Readout-Segmented Echo-Planar Imaging
title_short Performance of Single-Shot Echo-Planar Imaging in Diffusion Tensor Imaging in Rat Sciatic Nerve Compared With Readout-Segmented Echo-Planar Imaging
title_sort performance of single-shot echo-planar imaging in diffusion tensor imaging in rat sciatic nerve compared with readout-segmented echo-planar imaging
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133884/
https://www.ncbi.nlm.nih.gov/pubmed/35645709
http://dx.doi.org/10.3389/fnins.2022.844408
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