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Analysis of a miR-148a Targetome in B Cell Central Tolerance
A microRNA (miRNA) often regulates the expression of hundreds of target genes. A fundamental question in the field of miRNA research is whether a miRNA exerts its biological function through regulating a small number of key targets or through small changes in the expression of hundreds of target gen...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134011/ https://www.ncbi.nlm.nih.gov/pubmed/35634349 http://dx.doi.org/10.3389/fimmu.2022.861655 |
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author | Ma, Fengge Zhan, Yating Bartolomé-Cabrero, Rocío Ying, Wei Asano, Masahide Huang, Zhe Xiao, Changchun González-Martín, Alicia |
author_facet | Ma, Fengge Zhan, Yating Bartolomé-Cabrero, Rocío Ying, Wei Asano, Masahide Huang, Zhe Xiao, Changchun González-Martín, Alicia |
author_sort | Ma, Fengge |
collection | PubMed |
description | A microRNA (miRNA) often regulates the expression of hundreds of target genes. A fundamental question in the field of miRNA research is whether a miRNA exerts its biological function through regulating a small number of key targets or through small changes in the expression of hundreds of target genes. We addressed this issue by performing functional analysis of target genes regulated by miR-148a. We previously identified miR-148a as a critical regulator of B cell central tolerance and found 119 target genes that may mediate its function. We selected 4 of them for validation and demonstrated a regulatory role for Bim, Pten, and Gadd45a in this process. In this study, we performed functional analysis of the other miR-148a target genes in in vitro and in vivo models of B cell central tolerance. Our results show that those additional target genes play a minimal role, if any, in miR-148a-mediated control of B cell central tolerance, suggesting that the function of miRNAs is mediated by a few key target genes. These findings have advanced our understanding of molecular mechanisms underlying miRNA regulation of gene expression and B cell central tolerance. |
format | Online Article Text |
id | pubmed-9134011 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91340112022-05-27 Analysis of a miR-148a Targetome in B Cell Central Tolerance Ma, Fengge Zhan, Yating Bartolomé-Cabrero, Rocío Ying, Wei Asano, Masahide Huang, Zhe Xiao, Changchun González-Martín, Alicia Front Immunol Immunology A microRNA (miRNA) often regulates the expression of hundreds of target genes. A fundamental question in the field of miRNA research is whether a miRNA exerts its biological function through regulating a small number of key targets or through small changes in the expression of hundreds of target genes. We addressed this issue by performing functional analysis of target genes regulated by miR-148a. We previously identified miR-148a as a critical regulator of B cell central tolerance and found 119 target genes that may mediate its function. We selected 4 of them for validation and demonstrated a regulatory role for Bim, Pten, and Gadd45a in this process. In this study, we performed functional analysis of the other miR-148a target genes in in vitro and in vivo models of B cell central tolerance. Our results show that those additional target genes play a minimal role, if any, in miR-148a-mediated control of B cell central tolerance, suggesting that the function of miRNAs is mediated by a few key target genes. These findings have advanced our understanding of molecular mechanisms underlying miRNA regulation of gene expression and B cell central tolerance. Frontiers Media S.A. 2022-05-12 /pmc/articles/PMC9134011/ /pubmed/35634349 http://dx.doi.org/10.3389/fimmu.2022.861655 Text en Copyright © 2022 Ma, Zhan, Bartolomé-Cabrero, Ying, Asano, Huang, Xiao and González-Martín https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Ma, Fengge Zhan, Yating Bartolomé-Cabrero, Rocío Ying, Wei Asano, Masahide Huang, Zhe Xiao, Changchun González-Martín, Alicia Analysis of a miR-148a Targetome in B Cell Central Tolerance |
title | Analysis of a miR-148a Targetome in B Cell Central Tolerance |
title_full | Analysis of a miR-148a Targetome in B Cell Central Tolerance |
title_fullStr | Analysis of a miR-148a Targetome in B Cell Central Tolerance |
title_full_unstemmed | Analysis of a miR-148a Targetome in B Cell Central Tolerance |
title_short | Analysis of a miR-148a Targetome in B Cell Central Tolerance |
title_sort | analysis of a mir-148a targetome in b cell central tolerance |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134011/ https://www.ncbi.nlm.nih.gov/pubmed/35634349 http://dx.doi.org/10.3389/fimmu.2022.861655 |
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